药学学报, 2019, 54(8): 1409-1416
李冰涛, 翟兴英, 李佳, 涂珺, 张启云, 徐国良, 黄丽萍, 邵峰, 朱卫丰, 刘荣华. 基于网络药理学葛根解热作用机制研究[J]. 药学学报, 2019, 54(8): 1409-1416.
LI Bing-tao, ZHAI Xing-ying, LI Jia, TU Jun, ZHANG Qi-yun, XU Guo-liang, HUANG Li-ping, SHAO Feng, ZHU Wei-feng, LIU Rong-hua. Network pharmacology-based study on mechanisms of antipyretic action of Pueraria radix[J]. Acta Pharmaceutica Sinica, 2019, 54(8): 1409-1416.

李冰涛1, 翟兴英1, 李佳2, 涂珺1, 张启云1, 徐国良1, 黄丽萍2, 邵峰2, 朱卫丰2, 刘荣华2
1. 江西中医药大学中医基础理论分化发展研究中心, 江西 南昌 330004;
2. 江西中医药大学药学院, 江西 南昌 330004
关键词:    葛根      发热      网络药理学      分子对接      生物过程      通路分析     
Network pharmacology-based study on mechanisms of antipyretic action of Pueraria radix
LI Bing-tao1, ZHAI Xing-ying1, LI Jia2, TU Jun1, ZHANG Qi-yun1, XU Guo-liang1, HUANG Li-ping2, SHAO Feng2, ZHU Wei-feng2, LIU Rong-hua2
1. Research Center for Differentiation and Development of TCM Basic Theory, Jiangxi University of TCM, Nanchang 330004, China;
2. School of Pharmacy, Jiangxi University of TCM, Nanchang 330004, China
This study was designed to explore the antipyretic mechanism of Pueraria radix. The method of network pharmacology was used to determine the known ingredients corresponding to Pueraria radix, predict the drug-related gene/protein targets, and analyze the interplay between key ingredients and targets. Biological Information Annotation Databases (DAVID) was used to enrich the biological processes and pathways. The result of network analysis was validated by molecular docking. It was found that 49 active ingredients of Pueraria radix not only regulate 21 targets (e.g. PTGS2, EGFR), but also affect 11 biological processes (e.g. oxidation-reduction process, prostaglandin synthesis, positive regulation of fever generation and inflammatory response) and 7 metabolic pathways (arachidonic acid metabolism, serotonergic synapse and HIF-1, et al). Molecular docking results showed that more than 65% of the active ingredients could be well docked with key targets, and the relevant literatures indicated that the active components could inhibit the expression of PTGS2, which means the result has a high reliability. These results indicated that Pueraria radix may carry its pyretic action via a "multi-ingredients-multitargets-multi-pathways" mode, which provides a scientific basis for further research and drug development.
Key words:    Pueraria radix    fever    network pharmacology    molecular docking    biology process    pathway analysis   
收稿日期: 2018-12-09
DOI: 10.16438/j.0513-4870.2018-1098
基金项目: 国家科技支撑计划(2017YFC1702902);江西省中医药科技计划项目(2018A309);江西中医药大学博士启动基金(2015BS010).
通讯作者: 刘荣华,Tel:86-791-87118992,E-mail:rhliu@163.com
Email: rhliu@163.com
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李冰涛  在本刊中的所有文章
翟兴英  在本刊中的所有文章
李佳  在本刊中的所有文章
涂珺  在本刊中的所有文章
张启云  在本刊中的所有文章
徐国良  在本刊中的所有文章
黄丽萍  在本刊中的所有文章
邵峰  在本刊中的所有文章
朱卫丰  在本刊中的所有文章
刘荣华  在本刊中的所有文章

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