药学学报, 2019, 54(9): 1597-1605
引用本文:
孟彦, 颜磊, 王琦, 向欢, 武嫣斐, 秦雪梅, 田俊生. 心理亚健康实验模型大鼠肠道菌群变化及其规律[J]. 药学学报, 2019, 54(9): 1597-1605.
MENG Yan, YAN Lei, WANG Qi, XIANG Huan, WU Yan-fei, QIN Xue-mei, TIAN Jun-sheng. Changes of gut microbiota in experimental model rats with psychological suboptimal health state[J]. Acta Pharmaceutica Sinica, 2019, 54(9): 1597-1605.

心理亚健康实验模型大鼠肠道菌群变化及其规律
孟彦1,2, 颜磊1,2, 王琦1,2, 向欢3, 武嫣斐4, 秦雪梅1,2, 田俊生1,2
1. 山西大学中医药现代研究中心, 山西 太原 030006;
2. 山西大学地产中药功效与物质研究与利用山西省重点实验室, 山西 太原 030006;
3. 山西大学体育学院, 山西 太原 030006;
4. 山西医科大学第一医院, 山西 太原 030001
摘要:
探究心理亚健康实验模型大鼠肠道菌群的变化及其规律。给予实验大鼠孤养、不确定性空瓶应激、昼夜紊乱等多种应激,模拟人类现实生活中遭遇各种应激事件所造成的心理亚健康状态。采用旷场、高架十字迷宫、血液生化等指标对模型进行评价,并采用16S rRNA基因高通量测序法研究该模型的肠道菌群变化及其规律。所有实验方案均经山西大学生物医学研究实验动物伦理委员会批准。与空白对照组比较,心理亚健康模型组大鼠体重增长缓慢(P<0.01),在高架十字迷宫的开臂次数比例和开臂时间比例明显减少(P<0.05),在旷场实验中的穿越格数和直立次数显著减少(P<0.05),但糖水偏爱率、血常规和血液生化等指标未发生显著变化。肠道菌群分析结果发现,心理亚健康模型大鼠肠道菌群多样性发生改变,10种菌群的相对丰度显著改变(P<0.05,P<0.01),包括厚壁菌门中Bacilli菌纲,Lactobacillales菌目,Lactobacillaceae菌科,Lactobacillus菌属;Clostridia菌纲,Clostridiales菌目,Blautia菌属;放线菌门中Coriobacteriales菌目,Coriobacteriaceae菌科,Adlercreutzia菌属。建立的心理亚健康模型大鼠能够在一定程度上反映人类的心理亚健康状态,通过研究实验模型大鼠的肠道菌群变化及其规律,为阐释心理亚健康状态的生理变化机制及制定相关干预措施提供重要参考依据。
关键词:    心理亚健康      实验大鼠      动物模型      肠道菌群     
Changes of gut microbiota in experimental model rats with psychological suboptimal health state
MENG Yan1,2, YAN Lei1,2, WANG Qi1,2, XIANG Huan3, WU Yan-fei4, QIN Xue-mei1,2, TIAN Jun-sheng1,2
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;
2. Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan 030006, China;
3. Physical Education Departments, Shanxi University, Taiyuan 030006, China;
4. First Hospital of Shanxi Medical University, Taiyuan 030001, China
Abstract:
This study aimed to explore changes of gut microbiota in experimental model rats with psychological suboptimal health state (PSHS). The experimental rats were given a variety of stresses, including orphaned, uncertain empty bottle stress, circadian disorder and other stresses to simulate PSHS caused by various stress events in human life. Open field test, elevated cross maze, blood biochemical indexes and other auxiliary models were used to evaluate the experimental animal model. Changes of gut microbiota were studied by 16S rRNA gene high-throughput sequencing. All experimental protocols were approved by the Animal Ethics Committee of the Biomedical Research Laboratory of Shanxi University. Compared with the control group, the body weight of PSHS group gained slowly (P<0.01). In addition, the numbers of crossings and rearings in the open field test, and the percentage of time and entries in the open arms, were significantly reduced compared to the control group (P<0.05). But there were no significant change in the percentage of sucrose preference, blood biochemical, blood routine examination and so on. Gut microbiota analyses showed that the diversity of gut microbiota in PSHS model rats changed, and the relative abundances of 10 species of gut microbiota changed significantly (P<0.05, P<0.01), including Bacilli, Lactobacillales, Lactobacillaceae, Lactobacillus, Clostridia, Clostridiales, Blautia, Coriobacteriales, Coriobacteriaceae, and Adlercreutzia. To some extent, the established PSHS model rats reflect the state of humans with PSHS. By studying the changes of gut microbiota in the animal model with PSHS, this study provided an important reference for explaining the physiological change due to PSHS and formulating relevant measures of intervention.
Key words:    psychological suboptimal health    experimental rat    animal model    gut microbiota   
收稿日期: 2019-01-10
DOI: 10.16438/j.0513-4870.2019-0035
基金项目: 国家自然科学基金资助项目(81441096);山西省应用基础研究资助项目(201701D121137);山西省科技攻关计划一般项目(201603D321077);山西省科技重点研发计划(201603D3113013);山西省科技创新重点团队(201605D131045-18);山西省重点实验室(201605D111004).
通讯作者: 田俊生,Tel:86-351-7019297,E-mail:jstian@sxu.edu.cn
Email: jstian@sxu.edu.cn
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