药学学报, 2019, 54(9): 1606-1611
引用本文:
闫岩, 张斯琳, 陈佳欣, 焦文君, 张学武. 人参皂苷CK通过抑制TGF-β1/Smads通路诱导人肝癌SMMC-7721细胞凋亡的作用[J]. 药学学报, 2019, 54(9): 1606-1611.
YAN Yan, ZHANG Si-lin, CHEN Jia-xin, JIAO Wen-jun, ZHANG Xue-wu. Ginsenoside CK induces apoptosis of human liver cancer SMMC-7721 cells through inhibition of TGF-β1/Smads signaling pathway[J]. Acta Pharmaceutica Sinica, 2019, 54(9): 1606-1611.

人参皂苷CK通过抑制TGF-β1/Smads通路诱导人肝癌SMMC-7721细胞凋亡的作用
闫岩, 张斯琳, 陈佳欣, 焦文君, 张学武
延边大学医学院, 吉林 延吉 133002
摘要:
探讨人参皂苷CK(ginsenoside,CK)通过抑制TGF-β1/Smads通路诱导人肝癌SMMC-7721细胞凋亡的作用。采用MTT法检测人参皂苷CK对人肝癌SMMC-7721细胞增殖的作用;流式细胞术检测细胞凋亡;Western blot技术分别检测加入人参皂苷CK、TGF-β1/Smads通路激活剂TGFβ1及抑制剂LY2109761后TGF-β1/Smads通路相关蛋白及凋亡相关蛋白的表达水平。结果显示,人参皂苷CK能够抑制人肝癌SMMC-7721细胞的增殖,诱导细胞凋亡,上调cleaved caspase-3的表达,下调Bcl-2/Bax值。检测TGF-β1/Smads通路发现,人参皂苷CK能够下调Smad2/3、p-Smad2/3和Smad4表达,促进Smad7表达;抑制TGFβ1诱导的Smad2/3、p-Smad2/3和Smad4上调,并促进cleaved caspase-3的表达,下调Bcl-2/Bax值;加入LY2109761后,Smad2/3、p-Smad2/3和Smad4表达量显著降低,而人参皂苷CK对抑制TGF-β1/Smads通路诱导的细胞凋亡没有显著影响,LY2109761组与LY2109761+人参皂苷CK组cleaved caspase-3的表达及Bcl-2/Bax值无明显变化。研究表明,人参皂苷CK可以诱导人肝癌SMMC-7721细胞凋亡,其作用机制与抑制TGF-β1/Smads通路有关。
关键词:    肝癌      人参皂苷CK      转化生长因子β1/Smads通路      细胞凋亡     
Ginsenoside CK induces apoptosis of human liver cancer SMMC-7721 cells through inhibition of TGF-β1/Smads signaling pathway
YAN Yan, ZHANG Si-lin, CHEN Jia-xin, JIAO Wen-jun, ZHANG Xue-wu
Yanbian University Medical College, Yanji 133002, China
Abstract:
This study aimed to investigate apoptosis induction of ginsenoside compound K (ginsenoside CK) in human liver cancer SMMC-7721 cells and the involvement of TGF-β1/Smads signaling pathway. MTT assay was used to detect cell viability following ginsenoside CK treatment in SMMC-7721 cells. Annexin V-FITC/PI assay was used to detect apoptosis. After ginsenoside CK, or TGF-β1/Smads pathway activator TGFβ1 and inhibitor LY2109761 treatment, the TGF-β1/Smads pathway proteins and apoptosis proteins were detected by Western blot. The results showed that ginsenoside CK inhibited the proliferation of SMMC-7721 cells in a dose-and time-dependent manner. Annexin V-FITC/PI showed that ginsenoside CK induced apoptosis in SMMC-7721 cells. Meanwhile, ginsenoside CK inhibited the expression of Smad2/3, p-Smad2/3, Smad4, but promoted Smad7 expression, cleavage of caspase-3 and down-regulated Bcl-2/Bax. Compared with TGFβ1 treatment alone, levels of Smad2/3, p-Smad2/3, Smad4 and the ratio of Bcl-2/Bax were down-regulated, whereas Smad7 or cleaved caspase-3 was up-regulated in the ginsenoside CK+TGF-β1 group. In addition, Smad2/3, p-Smad2/3 and Smad4 expression were decreased in LY2109761 group. Compared with LY2109761 group, cleaved caspase-3 expression and Bcl-2/Bax have no significant change in ginsenoside CK+LY2109761 group. Taken together, our results showed that ginsenoside CK induced apoptosis in SMMC-7721 cells, and such induction is related to inhibiting TGF-β1/Smads signaling pathway.
Key words:    hepatocellular carcinoma    ginsenoside CK    TGF-β1/Smads signaling    apoptosis   
收稿日期: 2019-01-26
DOI: 10.16438/j.0513-4870.2019-0088
基金项目: 国家自然科学基金资助项目(81760728).
通讯作者: 张学武,Tel:86-433-2435102,Fax:86-433-2435104,E-mail:zhangxuewu@ybu.edu.cn
Email: zhangxuewu@ybu.edu.cn
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