药学学报, 2019, 54(9): 1661-1666
引用本文:
张影, 许华容, 李军山, 高晗, 毕开顺, 李清. 基于一测多评法的茵陈标准汤剂中8种成分同时定量研究[J]. 药学学报, 2019, 54(9): 1661-1666.
ZHANG Ying, XU Hua-rong, LI Jun-shan, GAO Han, BI Kai-shun, LI Qing. Simultaneous quantitative analyses of eight components in Artemisia capillaris Thunb standard decoction based on a quantitative method of multi-components with a single-marker[J]. Acta Pharmaceutica Sinica, 2019, 54(9): 1661-1666.

基于一测多评法的茵陈标准汤剂中8种成分同时定量研究
张影1, 许华容1, 李军山2, 高晗2, 毕开顺1, 李清1
1. 沈阳药科大学药学院, 辽宁 沈阳 110016;
2. 神威药业集团有限公司, 河北 石家庄 051430
摘要:
建立同时测定茵陈标准汤剂中的8种成分新绿原酸、绿原酸、咖啡酸、隐绿原酸、1,3-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸的一测多评方法。采用超高效液相色谱法(UHPLC),Waters Cortecs T3(2.1 mm×100 mm,2.7 μm)色谱柱,乙腈-0.05%磷酸水溶液为流动相,梯度洗脱,流速0.5 mL·min-1,柱温30℃。建立新绿原酸、咖啡酸、隐绿原酸、1,3-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸与内参物绿原酸的相对校正因子,并通过相对校正因子计算茵陈标准汤剂中8种成分的含量。同时,与外标法的测定结果进行对比,验证所建立的一测多评法的合理性、可行性和重复性。结果表明,新绿原酸、咖啡酸、隐绿原酸、1,3-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸与内参物绿原酸的相对校正因子分别为0.928 0、0.546 2、1.099 8、0.872 1、1.086 8、0.739 2和1.056 6;一测多评法的测定结果与外标法的测定结果无显著性差异。建立的一测多评法可作为一种简便、准确的质量评价模式,用于茵陈标准汤剂中新绿原酸、绿原酸、咖啡酸、隐绿原酸、1,3-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸的含量测定。
关键词:    一测多评法      相对校正因子      茵陈标准汤剂      超高效液相色谱法      含量测定     
Simultaneous quantitative analyses of eight components in Artemisia capillaris Thunb standard decoction based on a quantitative method of multi-components with a single-marker
ZHANG Ying1, XU Hua-rong1, LI Jun-shan2, GAO Han2, BI Kai-shun1, LI Qing1
1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;
2. Shenwei Pharmaceutical Group Co., Ltd., Shijiazhuang 051430, China
Abstract:
A quantitative analytical method for multi-components with a single-marker (QAMS) was established for simultaneous determination of neochlorogenic acid, chlorogenic acid, caffeic acid, cryptochlorogenic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in Artemisia capillaris Thunb standard decoction. The separation was performed on a Waters CORTECS T3 column (2.1 mm×100 mm, 2.7 μm), with the mobile phase consisting of 0.05% phosphate acid solution-acetonitrile for gradient elution. The column temperature was 30℃, and flow rate was 0.5 mL·min-1. Using chlorogenic acid as an internal reference, the relative correlation factors of neochlorogenic acid, caffeic acid, cryptochlorogenic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid were calculated following UHPLC, as 0.928 0, 0.546 2, 1.099 8, 0.872 1, 1.086 8, 0.739 2, 1.056 6, respectively. The results were compared with those obtained by the external standard method to verify the feasibility, rationality and repeatability of QAMS method. There was no significant difference in assay results between QAMS and the external standard method. In conclusion, the QAMS method is accurate and feasible, and could be used to determine the content such as neochlorogenic acid, caffeic acid, cryptochlorogenic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in Artemisia capillaris Thunb standard decoction.
Key words:    quantitative analysis of multi-components with a single-marker    relative correlation factor    Artemisia capillaris Thunb standard decoction    UHPLC    content determination   
收稿日期: 2019-04-17
DOI: 10.16438/j.0513-4870.2019-0292
通讯作者: 李清,Tel:86-24-43520589,E-mail:lqyxm@hotmail.com
Email: lqyxm@hotmail.com
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