药学学报, 2019, 54(11): 1909-1917
引用本文:
李伟霞, 王晓艳, 唐进法, 张书琦, 王炎, 张辉, 刘现磊, 孟祥乐, 李学林, 唐于平. 基于PLS-DA和多指标综合指数法研究当归-川芎药对对急性血瘀大鼠血清中血管活性物质和黏附分子的影响[J]. 药学学报, 2019, 54(11): 1909-1917.
LI Wei-xia, WANG Xiao-yan, TANG Jin-fa, ZHANG Shu-qi, WANG Yan, ZHANG Hui, LIU Xian-lei, MENG Xiang-le, LI Xue-lin, TANG Yu-ping. Comparative study on the effect of Danggui-Chuanxiong herb pair on vasoactive substances and adhesion molecules in the serum of acute blood stasis in rats using PLS-DA and multi-attribute comprehensive index methods[J]. Acta Pharmaceutica Sinica, 2019, 54(11): 1909-1917.

基于PLS-DA和多指标综合指数法研究当归-川芎药对对急性血瘀大鼠血清中血管活性物质和黏附分子的影响
李伟霞1, 王晓艳1, 唐进法1,2, 张书琦2, 王炎2, 张辉1, 刘现磊1, 孟祥乐1, 李学林1,2, 唐于平3
1. 河南中医药大学第一附属医院, 中药临床评价技术河南省工程实验室, 河南 郑州 450000;
2. 河南中医药大学药学院, 河南 郑州 450046;
3. 陕西中医药大学, 陕西省中医药管理局中药配伍重点研究室, 陕西 西安 712046
摘要:
比较评价当归-川芎药对不同配比(1:0、3:2、1:1、2:3、0:1)和不同制法(水提、醇提和先水提后醇提)样品对急性血瘀大鼠血清中血管活性物质和内皮细胞黏附分子的影响。本研究采用冰水浴和皮下注射盐酸肾上腺素共同复制急性血瘀大鼠模型,采用酶联免疫吸附实验检测大鼠血清中血管活性物质(花生四烯酸代谢物、凝血-纤溶系统指标)和黏附分子等相关指标的表达,采用斯皮尔曼方法对检测指标进行相关性分析,采用偏最小二乘法-判别分析(PLS-DA)和多指标综合指数法综合评价当归-川芎药对不同配比、不同制法样品对急性血瘀大鼠血清中血管活性物质和黏附分子的总效应。实验方案经河南中医药大学第一附属医院动物实验伦理委员会批准。结果表明,当归-川芎药对1:1先水提后醇提组对急性血瘀大鼠血清中血管活性物质和黏附分子的改善总效应最强(总效应值为6.96);提取方法相同时,当归-川芎药对1:1的整体作用较好;配比相同时,当归-川芎先水提后醇提给药组和醇提给药组的总效应优于水提给药组。当归-川芎配伍能明显改善血瘀大鼠血清中与血瘀相关的血管活性物质及黏附分子表达,但配比和制法不同,其作用强度亦存在差异,为该药对临床合理应用提供依据,也为该药对治疗血瘀相关疾病的深入研究奠定基础。
关键词:    当归      川芎      药对      偏最小二乘法-判别分析      多指标综合指数法      活血化瘀     
Comparative study on the effect of Danggui-Chuanxiong herb pair on vasoactive substances and adhesion molecules in the serum of acute blood stasis in rats using PLS-DA and multi-attribute comprehensive index methods
LI Wei-xia1, WANG Xiao-yan1, TANG Jin-fa1,2, ZHANG Shu-qi2, WANG Yan2, ZHANG Hui1, LIU Xian-lei1, MENG Xiang-le1, LI Xue-lin1,2, TANG Yu-ping3
1. Henan Province Engineering Laboratory for Clinical Evaluation Technology of Chinese Medicine, the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, China;
2. Henan University of Chinese Medicine, Zhengzhou 450046, China;
3. Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, Shanxi University of Chinese Medicine, Xi'an 712046, China
Abstract:
We evaluated the effects of Danggui-Chuanxiong (GX) herb pair with different proportions (1:0, 3:2, 1:1, 2:3, 0:1) and preparation methods (water extract W, alcohol extract A, and water-alcohol extracts WA) on vasoactive substances and endothelial cell adhesion molecules in the serum of acute blood stasis in rats. An acute blood stasis model was co-replicated by ice water bath and subcutaneous injection of epinephrine hydrochloride in rats. The expressions of vasoactive substances (arachidonic acid metabolites, coagulation-fibrin system index) and adhesion molecules in the serum were detected by enzyme linked immunosorbent assay method; the Spearman method was used to analyze the correlation of those detection indicators; the partial least squares-discriminant analysis and multi-attribute comprehensive index method were used to comprehensively evaluate the total effect of GX herb pair samples with different proportions and preparation methods on vasoactive substances and adhesion molecules. The experimental scheme was approved by the Animal Experimental Ethics Committee of the First Affiliated Hospital of Henan University of Chinese Medicine. The results showed that GX 1:1_WA had the strongest effect on the improvement of vasoactive substances and adhesion molecules in the serum of acute blood stasis in rats (the total effect value was 6.96). When extraction method was same, the overall effect of GX 1:1 had better effect than that of other proportions; when the proportion of GX was same, the total effects of GX_WA and GX_A were better than GX_W. The combination of Danggui and Chuanxiong can significantly improve the expressions of vasoactive substances and adhesion molecules in the serum of blood stasis in rats. But the action strength of GX herb pairs was different when the proportions and preparations of GX herb pair were different. These findings provide a basis for clinical rational application of GX herb pair, and lay the foundation for in-depth research on GX herb pair for treatment of blood stasis related diseases.
Key words:    Danggui    Chuanxiong    herb pair    partial least squares discrimination analysis    multi-attribute comprehensive index method    activating blood circulation to dissipate blood stasis   
收稿日期: 2019-04-02
DOI: 10.16438/j.0513-4870.2019-0227
基金项目: 国家自然科学基金-河南人才培养联合基金资助项目(U1504827).
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