药学学报, 2019, 54(11): 2049-2054
引用本文:
杜琳, 黄清东, 陈聪地, 韩涛, 张琦, 杜伟. 蔓菁中两个新的三萜化合物及其抗癌活性研究[J]. 药学学报, 2019, 54(11): 2049-2054.
DU Lin, HUANG Qing-dong, CHEN Cong-di, HAN Tao, ZHANG Qi, DU Wei. Anti-cancer activity of two new triterpenes from Brassica rapa[J]. Acta Pharmaceutica Sinica, 2019, 54(11): 2049-2054.

蔓菁中两个新的三萜化合物及其抗癌活性研究
杜琳1,2, 黄清东3, 陈聪地1,2, 韩涛1,2, 张琦1,2, 杜伟4
1. 成都师范学院化学与生命科学学院, 四川 成都 611130;
2. 成都师范学院功能分子研究所, 四川 成都 611130;
3. 成都百特万合医药科技有限公司; 四川 成都 611130;
4. 郑州大学第一附属医院神经外科, 河南 郑州 450052
摘要:
综合运用大孔吸附树脂、硅胶柱色谱、半制备型高效液相及Sephadex LH-20凝胶柱色谱等各种色谱技术,对蔓菁Brassicarapa L.乙醇提取物的化学成分进行系统研究,共分离得到4个三萜、3个黄酮和1个甾体类化合物。根据其理化性质和高分辨及二维核磁等波谱数据鉴定化合物的结构,其中12为新的三萜类化合物,37首次从该植物中分离得到。细胞毒活性显示12对5种常见的癌细胞均显示一定的抑制作用,尤其是对HL-60具有显著的细胞毒活性,其IC50分别为5.87和10.32 μmol·L-1
关键词:    藏药      蔓菁      化学成分      细胞毒活性     
Anti-cancer activity of two new triterpenes from Brassica rapa
DU Lin1,2, HUANG Qing-dong3, CHEN Cong-di1,2, HAN Tao1,2, ZHANG Qi1,2, DU Wei4
1. College of Chemistry and Life Science, Chengdu Normal University, Chengdu 611130, China;
2. Institute of Functional Molecules, Chengdu Normal University, Chengdu 611130, China;
3. Chengdu Better Pharmaceutical Technology Co., Ltd., Chengdu 611130, China;
4. Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Abstract:
The chemical constituents from Brassica rapa were identified by various chromatographic techniques including silica gel, reversed-phase silica gel, macroporous resin and Sephadex LH-20 column chromatography. Eight compounds were isolated from this plant. The isolated compounds were elucidated by physicochemical properties and spectroscopic methods, including extensive 1D, 2D-NMR, and HR-ESI-MS techniques. Compounds 1, 2 are new triterpenoids and 3-7 were isolated for the first time from Brassica rapa. The cytotoxic effect of compounds 1 and 2 were tested by MTT assay against five cancer cell lines. The result showed that all compounds exhibit growth inhibition for the cancer lines. Compound 1 has an IC50 value of 5.87 μmol·L-1 for growth inhibition of leukemia cell line HL-60, and IC50 value for compound 2 was 10.32 μmol·L-1.
Key words:    Tibetan material herb    Brassica rapa    chemical constituent    cytotoxic activity   
收稿日期: 2019-06-10
DOI: 10.16438/j.0513-4870.2019-0454
基金项目: 四川省科技厅重点研发项目(2019YFS0096).
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