药学学报, 2019, 54(11): 2055-2058
引用本文:
周健, 李创军, 陈芳有, 吴练秋, 马洁, 杨敬芝, 张东明. 截叶铁扫帚中黄酮类成分研究[J]. 药学学报, 2019, 54(11): 2055-2058.
ZHOU Jian, LI Chuang-jun, CHEN Fang-you, WU Lian-qiu, MA Jie, YANG Jing-zhi, ZHANG Dong-ming. Flavonoids from the aerial parts of Lespedeza cuneata[J]. Acta Pharmaceutica Sinica, 2019, 54(11): 2055-2058.

截叶铁扫帚中黄酮类成分研究
周健1,2, 李创军1, 陈芳有3, 吴练秋1, 马洁1, 杨敬芝1, 张东明1
1. 中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050;
2. 南昌大学第一附属医院药学部, 江西 南昌 330006;
3. 江西中医药大学药学院, 江西 南昌 330004
摘要:
运用硅胶柱色谱、中压液相色谱、制备液相色谱等多种色谱技术,对截叶铁扫帚70%乙醇提取物进行了分离纯化。从中分离得到了5个黄酮类化合物,根据理化性质和波谱数据鉴定为7-O-glucosyllaburnetin(1)、山柰酚-3-O-β-D-半乳糖苷(2)、山柰酚-3-O-α-L-鼠李糖苷(3)、牡荆素(4)和异牡荆素(5)。其中化合物1为新化合物,化合物23均为首次从截叶铁扫帚中分离得到。采用体外靶向xbp1高通量双荧光素酶报告基因药物筛选模型,对截叶铁扫帚所含主要成分进行了xbp1转录激活作用评价,结果发现化合物1具有一定的xbp1转录激活作用,其作用强度为空载体对照组的1.80倍。
关键词:    截叶铁扫帚      黄酮类成分      x-盒结合蛋白-1      转录激活     
Flavonoids from the aerial parts of Lespedeza cuneata
ZHOU Jian1,2, LI Chuang-jun1, CHEN Fang-you3, WU Lian-qiu1, MA Jie1, YANG Jing-zhi1, ZHANG Dong-ming1
1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;
2. Department of Pharmacy, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China;
3. School of pharmacy, Jiangxi University of Traditional Chinese Medicines, Nanchang 330004, China
Abstract:
The chemical constituents of the aerial parts of Lespedeza cuneata (Dum. Cour.) G. Don were investigated using chromatographic techniques, such as silica gel, reversed phase MPLC and preparative HPLC. Five compounds were isolated and their structures were elucidated by spectroscopic data and physicochemical properties, which were identified as 7-O-glucosyllaburnetin (1), kaempferol-3-O-β-D-galactopyranoside (2), kaempferol-3-O-α-L-rhamnoside (3), vitexin (4), and isovitexin (5). Among those, compound 1 is a new compound, compounds 2-3 were isolated from this plant for the first time. Compounds 1-5 were tested for their anti-ulcerative colitis activity by dual luciferase report gene assay targeting xbp1. Compared with control group, compound 1 showed a certain activity on activating the transcription of xbp1, with its relative activating ratio being 1.80 times.
Key words:    Lespedeza cuneata    flavonoid    x-box-binding protein 1    transcriptional activation   
收稿日期: 2019-07-25
DOI: 10.16438/j.0513-4870.2019-0599
基金项目: 国家自然科学基金资助项目(81560632);江西省卫生计生委科技计划项目(20175135);江西省卫计委中医药科技计划项目(2017A299).
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