药学学报, 2019, 54(11): 2059-2063
引用本文:
孙晨, 谢浙裕, 吴永江. 益母草HPLC指纹图谱及多成分含量测定方法研究[J]. 药学学报, 2019, 54(11): 2059-2063.
SUN Chen, XIE Zhe-yu, WU Yong-jiang. Fingerprint analysis and multi-component determination of Leonurus japonicus by high performance liquid chromatography[J]. Acta Pharmaceutica Sinica, 2019, 54(11): 2059-2063.

益母草HPLC指纹图谱及多成分含量测定方法研究
孙晨1,2, 谢浙裕2, 吴永江1
1. 浙江大学药学院, 浙江 杭州 310058;
2. 绍兴市食品药品检验研究院, 浙江 绍兴 312071
摘要:
建立益母草的HPLC指纹图谱及多成分含量同时测定方法,为益母草质量综合评价和控制提供参考。益母草用70%乙醇水浴回流提取2 h,提取物采用HPLC法分析,色谱柱为Kromasil C18(250mm×4.6 mm,5 μm),流动相为乙腈-0.1%甲酸,梯度洗脱,流速为1.0 mL·min-1,柱温30℃,检测波长为280 nm。25批样品得到共有峰12个,确认了其中5个峰对应的化学成分。丁香酸、盐酸益母草碱、芦丁、金丝桃苷和异槲皮苷分别在进样量0.426 1~85.22ng(r=0.999 9)、7.948~1590 ng(r=0.999 3)、10.20~2040 ng(r=1.000 0)、2.018~403.6ng(r=0.999 9)、8.704~1741 ng(r=0.999 9)内呈良好的线性关系;平均回收率分别为99.0%、97.6%、97.4%、96.9%和98.5%,RSD分别为1.1%、1.8%、1.4%、1.5%和1.3%。该指纹图谱特征性明显,并可同时测定5个成分的含量。
关键词:    益母草      指纹图谱      多成分含量测定     
Fingerprint analysis and multi-component determination of Leonurus japonicus by high performance liquid chromatography
SUN Chen1,2, XIE Zhe-yu2, WU Yong-jiang1
1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;
2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China
Abstract:
An HPLC fingerprint and multi-component determination method of Leonurus japonicus was established for comprehensive evaluation and quality control of Leonurus japonicus. The sample was incubated in 70% ethanol in a water bath for 2 h, and the extract was analyzed by HPLC using Kromasil C18 column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution. The flow rate was 1.0 mL·min-1. The temperature of column was 30℃. The detection wavelength was 280 nm. HPLC fingerprint of characteristic components of Leonurus japonicus was established. There were 12 common peaks among 25 batches of samples, and 5 of them were identified and determined. Syringic acid, leonurine hydrochloride, rutin, hyperoside or isoquercitrin showed a good linearity in the ranges of 0.426 1-85.22 ng (r=0.999 9), 7.948-1 590 ng (r=0.999 3), 10.20-2 040 ng (r=1.000 0), 2.018-403.6 ng (r=0.999 9), or 8.704-1 741 ng (r=0.999 9), respectively. The average recoveries were 99.0%, 97.6%, 97.4%, 96.9% and 98.5% with RSD of 1.1%, 1.8%, 1.4%, 1.5% and 1.3%, respectively. The HPLC characteristic fingerprint of Leonurus japonicus was specific, and this method can simultaneously determine the content of 5 components.
Key words:    Leonurus japonicus    fingerprint    multi-component determination   
收稿日期: 2019-05-24
DOI: 10.16438/j.0513-4870.2019-0407
基金项目: 名贵中药资源可持续利用能力建设项目(2060302-1508-04);国家科技重大专项“重大新药创制”项目(2018ZX09201-010)资助.
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