药学学报, 2019, 54(11): 2083-2088
引用本文:
王菲菲, 刘杰, 何风艳, 张南平, 何轶, 李静, 张聿梅, 郑健, 马双成. 薄荷属3种植物及变种的性状鉴别、化学成分分析及DNA条形码研究比较[J]. 药学学报, 2019, 54(11): 2083-2088.
WANG Fei-fei, LIU Jie, HE Feng-yan, ZHANG Nan-ping, HE Yi, LI Jing, ZHANG Yu-mei, ZHENG Jian, MA Shuang-cheng. Comparative study for morphological identification, chemical component analysis, and DNA barcode determination of three plants and their varieties in Genus Mentha[J]. Acta Pharmaceutica Sinica, 2019, 54(11): 2083-2088.

薄荷属3种植物及变种的性状鉴别、化学成分分析及DNA条形码研究比较
王菲菲, 刘杰, 何风艳, 张南平, 何轶, 李静, 张聿梅, 郑健, 马双成
中国食品药品检定研究院, 北京 100050
摘要:
对薄荷属植物薄荷、胡椒薄荷、留兰香及其栽培变种进行性状鉴定、化学成分鉴定和分子生物学鉴定,比较不同分析方法对薄荷属植物鉴别结果的差异,以了解药用薄荷的基源情况,确保药用薄荷在临床中的正确使用。采用性状鉴别、GC-MS/MS测定挥发性化学成分和聚合酶链式反应(PCR)对22批次薄荷属样品进行分析,确定它们的种间归属情况。在性状鉴别中,“轮伞花序腋生”被认为是薄荷典型特征之一;在化学成分鉴别中,香芹酮可作为鉴别留兰香的指标性成分;在基因聚类分析中,22批次样品主要归属为2个分枝,薄荷和留兰香分别属于2个不同分枝中,但胡椒薄荷及部分杂交种则在两个分枝中均有分布。采用性状、化学成分和基因序列分析3种方法对22批次薄荷属植物进行分析,3种不同分类方法的分类结果具有一定的相关性,但每种方法均有局限性,且薄荷杂交现象较为常见,单一方法鉴别存在一定困难。
关键词:    薄荷      留兰香      胡椒薄荷      性状鉴别      化学成分分析      DNA条形码     
Comparative study for morphological identification, chemical component analysis, and DNA barcode determination of three plants and their varieties in Genus Mentha
WANG Fei-fei, LIU Jie, HE Feng-yan, ZHANG Nan-ping, HE Yi, LI Jing, ZHANG Yu-mei, ZHENG Jian, MA Shuang-cheng
National Institutes for Food and Drug Control, Beijing 100050, China
Abstract:
The morphological identification, chemical component analysis, and DNA barcode determination were investigated on Genus Mentha (including Mentha haplocalyx Briq., Mentha piperita Linn., Mentha spicata Linn. and Mentha cultivated) in order to reveal the origin of Herba Mentha as a drug, and ensure the accuracy in clinic application. The morphological characters, chemical composition analysis by GC-MS/MS and DNA content measure by polymerase chain reaction (PCR) were reported in this study for inter- or intra- species divergence. Based on the morphology, axillary verticillasters was recognized as the typical character for Mentha haplocalyx Briq. Carvone was used as an index component for chemical composition analysis of Mentha spicata Linn. Gene clustering analysis divided 22 batches of samples into two molecular groups. Mentha haplocalyx Briq. is distinguishably different from Mentha spicata Linn. Mentha piperita Linn. and other cultivated plants were distributed between these two species. The results obtained by morphological identification, chemical composition analysis, and DNA barcode determination show good correlations, but each identification method has its limit. In view of the fact that hybridization of the plants in Genus Mentha is common, identification relying on only one method is not recommended.
Key words:    Mentha haplocalyx Briq.    Mentha spicata Linn.    Mentha piperita Linn.    morphological identification    chemical compound analysis    DNA barcode   
收稿日期: 2019-05-20
DOI: 10.16438/j.0513-4870.2019-0395
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