药学学报, 2019, 54(12): 2282-2285
引用本文:
孙兴妍, 马洁, 李创军, 黄积武, 王楠, 沈苍颉, 张东明. 黄皮中的新呋喃香豆素[J]. 药学学报, 2019, 54(12): 2282-2285.
SUN Xing-yan, MA Jie, LI Chuang-jun, HUANG Ji-wu, WANG Nan, SHEN Cang-jie, ZHANG Dong-ming. A new furocoumarin from the stems of Clausena lansium[J]. Acta Pharmaceutica Sinica, 2019, 54(12): 2282-2285.

黄皮中的新呋喃香豆素
孙兴妍, 马洁, 李创军, 黄积武, 王楠, 沈苍颉, 张东明
中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050
摘要:
利用HPD-100大孔树脂柱色谱、硅胶柱色谱、ODS柱色谱等分离纯化方法,从黄皮[Clausena lansium (Lour.)Skeels]中分离得到3个呋喃香豆素化合物,并综合运用波谱学分析方法对化合物进行结构测定。确定其结构分别为:8-[(2S,3S,6E)-2,3-epoxy-3,7-dimethyl-oct-6-enyloxy]psoralen(1)、8-(7',8'-epoxygeranyloxy)psoralen(2)和8-[(2E)-6-oxo-3,7-dimethyloct-2-enyloxy]psoralen(3)。其中,化合物1为新的呋喃香豆素。对化合物1~3进行肿瘤细胞毒活性测试,结果显示,化合物2对人大细胞肺癌细胞(H460)具有抑制活性(IC50=43.94 μmol·L-1);化合物3对人宫颈癌细胞(HeLa)具有抑制活性(IC50=33.76 μmol·L-1)。
关键词:    黄皮      化学成分      呋喃香豆素      细胞毒活性     
A new furocoumarin from the stems of Clausena lansium
SUN Xing-yan, MA Jie, LI Chuang-jun, HUANG Ji-wu, WANG Nan, SHEN Cang-jie, ZHANG Dong-ming
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Abstract:
One undescribed and two known furocoumarins were isolated from the stems of the Clausena lansium through a series of isolation and purification approaches including HPD-100 macroporous resin column, silica gel, reverse phase C18 and so on. Their structures were determined to be 8-[(2S,3S,6E)-2,3-epoxy-3,7-dimethyl-oct-6-enyloxy] psoralen (1), 8-(7',8'-epoxygeranyloxy) psoralen (2) and 8-[(2E)-6-oxo-3,7-dimethyloct-2-enyloxy] psoralen (3) by spectroscopic methods. Compound 1 is a new furocoumarin. Compound 2 showed cytotoxicity to H460 (IC50=43.94 μmol·L-1) and compound 3 showed cytotoxicity to HeLa (33.76 μmol·L-1) through the cytotoxic tests against five human cancer cell lines (H460, H7402, HCT-8, HeLa and MCF-7) for all compounds.
Key words:    Clausena lansium    chemical constituents    furocoumarin    cytotoxicity   
收稿日期: 2019-07-10
DOI: 10.16438/j.0513-4870.2019-0545
基金项目: 北京市自然科学基金青年项目(7194299);国家自然科学基金资助项目(NNSFC81730093).
通讯作者: 张东明,Tel/Fax:86-10-63165227,E-mail:zhangdm@imm.ac.cn
Email: zhangdm@imm.ac.cn
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