药学学报, 2019, 54(12): 2289-2295
刘玉婷, 蒋且英, 黄国勇, 陈绪龙, 廖正根. LC-MS法研究黄芪对附子6种乌头生物碱大鼠药代动力学的影响[J]. 药学学报, 2019, 54(12): 2289-2295.
LIU Yu-ting, JIANG Qie-ying, HUANG Guo-yong, CHEN Xu-long, LIAO Zheng-gen. Determining the effect of Huangqi on the pharmacokinetics of six different alkaloids from Fuzi in rats by LC-MS[J]. Acta Pharmaceutica Sinica, 2019, 54(12): 2289-2295.

刘玉婷1, 蒋且英2, 黄国勇1, 陈绪龙1, 廖正根1
1. 江西中医药大学现代中药制剂教育部重点实验室, 江西 南昌 330004;
2. 江西中医药大学实验动物科技中心, 江西 南昌 330004
研究黄芪-附子配伍对附子3种单酯型生物碱(苯甲酰次乌头原碱BHA、苯甲酰新乌头原碱BMA、苯甲酰乌头原碱BAC)和3种双酯型生物碱(次乌头碱HA、新乌头碱MA、乌头碱AC)的大鼠药代动力学的影响。采用LC-MS测定大鼠单次给药后不同时间血浆中药物浓度,DAS 3.3求算药物的药代动力学参数,SPSS 13.0软件分析附子配伍黄芪前后主要药动学参数差异。配伍黄芪后,6种生物碱的AUC0-t和AUC0-∞均显著降低,除HA外的5种生物碱的CLz/F升高;3种单酯型生物碱Cmax降低,tmax延长;BMA和MA的分布容积(Vz/F)增大。黄芪可以抑制附子生物碱的吸收,诱导附子生物碱向全身分布,并加快附子生物碱在体内的消除。本研究中动物实验方案已获得江西中医药大学实验动物伦理委员会的批准。
关键词:    附子      黄芪      药代动力学      LC-MS     
Determining the effect of Huangqi on the pharmacokinetics of six different alkaloids from Fuzi in rats by LC-MS
LIU Yu-ting1, JIANG Qie-ying2, HUANG Guo-yong1, CHEN Xu-long1, LIAO Zheng-gen1
1. Key Laboratory of Modern Preparation of Chinese Materia Medica, Ministry of Education, Nanchang 330004, China;
2. Laboratory Animal Science and Technology Center of Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
This study aimed to investigate the influence of combinating Huangqi with Fuzi on the pharmacokinetics of six Aconitum alkaloids, i.e. aconitine (AC), hypaconitine (HA), mesaconitine (MA), benzoylaconine (BAC), benzoylhypaconine (BHA) and benzoylmesaconine (BMA). The plasma concentrations of the drugs were determined by LC-MS for dose response and time dependent curves. The pharmacokinetic parameters were calculated by DAS 3.3, and SPSS 12.0 was used to analyze the differences of main pharmacokinetic parameters between the two groups. Comparing with Fuzi group, the AUC0-t and AUC0-∞ of six alkaloids in Fuzi-Huangqi group was significantly decreased, the CLz/F of six alkaloids except HA was significantly increased; the Cmax was decreased and the tmax was prolonged in 3 monoester alkaloids, and the apparent volume of distribution of BMA and MA (Vz/F) increases. These data indicated that Huangqi can inhibit the absorption of aconite alkaloids, induce the distribution of aconite alkaloids to the whole body, and accelerate the elimination of aconite alkaloids. The animal experiment scheme in this study has been approved by the Experimental Animal Ethics Committee of Jiangxi University of Traditional Chinese Medicine.
Key words:    Fuzi    Huangqi    pharmacokinetics    LC-MS   
收稿日期: 2019-05-17
DOI: 10.16438/j.0513-4870.2019-0294
基金项目: 国家自然科学基金资助项目(81660757);江西省主要学科学术和技术带头人培养计划(20162BCB22015);江西省卫生计生委科技计划(20181140);九江市科技计划(2017109).
通讯作者: 廖正根,Tel:86-791-87118658,E-mail:lyzlyg@163.com
Email: lyzlyg@163.com
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刘玉婷  在本刊中的所有文章
蒋且英  在本刊中的所有文章
黄国勇  在本刊中的所有文章
陈绪龙  在本刊中的所有文章
廖正根  在本刊中的所有文章

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