药学学报, 2019, 54(12): 2296-2302
引用本文:
阴奇材, 陈聪聪, 田俊生, 高晓霞, 秦雪梅, 杜冠华, 周玉枝. 基于UPLC-QE-Orbitrap-MS技术的柴胡-白芍药对血清药物化学分析[J]. 药学学报, 2019, 54(12): 2296-2302.
YIN Qi-cai, CHEN Cong-cong, TIAN Jun-sheng, GAO Xiao-xia, QIN Xue-mei, DU Guan-hua, ZHOU Yu-zhi. Analysis of serum pharmacochemistry from Radix Bupleuri-Radix Paeoniae Alba herb using UPLC-QE-Orbitrap-MS technology[J]. Acta Pharmaceutica Sinica, 2019, 54(12): 2296-2302.

基于UPLC-QE-Orbitrap-MS技术的柴胡-白芍药对血清药物化学分析
阴奇材1,2, 陈聪聪1,2, 田俊生1, 高晓霞1, 秦雪梅1, 杜冠华1,3, 周玉枝1
1. 山西大学中医药现代研究中心, 山西 太原 030006;
2. 山西大学化学化工学院, 山西 太原 030006;
3. 中国医学科学院、北京协和医学院药物研究所, 北京 100050
摘要:
对柴胡-白芍药对的血清药物化学进行分析,探讨柴胡-白芍药对口服吸收后的入血成分。采用UPLC-QE-Orbitrap-MS技术,以及Compound Discover 2.0软件分析,比较药对提取液、空白血清、含药血清的图谱,根据质谱给出的精确分子量以及二级碎片信息,并结合参考文献以及对照品信息,找出口服药对后的入血成分。动物实验获得山西大学伦理委员会的批准。结果显示,大鼠口服给药柴胡-白芍药对后,共在血清中检测到55个移行成分,其中16个为原形成分,39个为原形成分的代谢产物。因此,基于UPLC-QE-Orbitrap-MS能快速、精确、全面地分析柴胡-白芍药对给药后的血中移行成分,为后期该药对的药效物质基础及配伍规律研究提供一定的借鉴与依据。
关键词:    柴胡      白芍      血清药物化学      UPLC-QE-Orbitrap-MS      原形成分      代谢产物     
Analysis of serum pharmacochemistry from Radix Bupleuri-Radix Paeoniae Alba herb using UPLC-QE-Orbitrap-MS technology
YIN Qi-cai1,2, CHEN Cong-cong1,2, TIAN Jun-sheng1, GAO Xiao-xia1, QIN Xue-mei1, DU Guan-hua1,3, ZHOU Yu-zhi1
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;
2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
3. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Abstract:
To analyze the chemical profile of Radix Bupleuri-Radix Paeoniae Alba in the serum, after oral absorption of the herbal pair, we used UPLC-QE-Orbitrap-MS technique and Compound Discover 2.0 software to compare the components in the extracts, and drug-containing serum. The map, based on the precise molecular weight and secondary fragment information given by the mass spectrometry, combined with the reference substance and reference information for, finding the chemical components in the blood after oral administration of the herbal pair in the rats. Animal experimentation was approved according to the Committee on the Ethics of Animal Experiments of Shanxi University. The results showed that a total of 55 components were detected in the serum, of which 16 were prototype components and 39 were metabolites of prototype components. UPLC-QE-Orbitrap-MS allows quick, accurate and comprehensive analysis of the blood components of the herb pair after oral administration. This preliminary study of the chemical components in the blood after herb pair ingestion provides a basis for the subsequent research on the pharmacodynamics and drug-drug interactions.
Key words:    Radix Bupleuri    Radix Paeoniae Alba    serum pharmacochemistry    UPLC-QE-Orbitrap-MS    prototype component    metabolite   
收稿日期: 2019-06-02
DOI: 10.16438/j.0513-4870.2019-0435
基金项目: 国家自然科学基金面上项目(81673572);国家“重大新药创新创制”科技重大专项课题(2017ZX09301047);山西省应用基础研究项目(201601D021164);山西省高校科技创新项目(2016120).
通讯作者: 周玉枝,Tel:86-351-7019178,E-mail:zhouyuzhi@sxu.edu.cn
Email: zhouyuzhi@sxu.edu.cn
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