药学学报, 2019, 54(12): 2335-2339
引用本文:
胡小松, 姜丹, 李婷, 许贞, 刘敬阁, 刘春生. 基于16S rRNA基因测序的陕西商洛区五灵脂菌群结构分析[J]. 药学学报, 2019, 54(12): 2335-2339.
HU Xiao-song, JIANG Dan, LI Ting, XU Zhen, LIU Jing-ge, LIU Chun-sheng. Diversity of microbiota composition of traditional Chinese medicine of Faeces Trogopterori from Shangluo, Shanxi province by sequence of the bacterial 16S rRNA gene[J]. Acta Pharmaceutica Sinica, 2019, 54(12): 2335-2339.

基于16S rRNA基因测序的陕西商洛区五灵脂菌群结构分析
胡小松1, 姜丹1, 李婷1, 许贞1, 刘敬阁2, 刘春生1
1. 北京中医药大学中药学院, 北京 102488;
2. 尚方堂中药有限公司, 甘肃 兰州 730030
摘要:
为分析中药材五灵脂中菌群微生物结构特点,本研究以陕西省商洛市复齿鼯鼠仿野生养殖地采集的10份复齿鼯鼠干燥粪便为研究对象,采用Illumina高通量测序技术对10份样品中微生物16S rRNA基因V3-V4区进行测序及生物信息学分析,并以COG和KEGG数据库为基础进行菌群功能预测分析。实验共获得285 218条长度分布在400~450 bp的高质量序列,经分析发现10份样本中微生物分布于不同的8个门、28个科、75个属共120种细菌。在门水平上,厚壁菌门(87.68%±2.68%)、拟杆菌门(7.62%±3.74%)为优势菌门,所有样本均显示出丰富的微生物多样性。功能预测表明菌群与能量代谢密切相关。实验结果说明五灵脂中携带的有益菌群可能为其有效成分之一,且样本中未检出致病菌。
关键词:    五灵脂      菌群结构      16sRNA测序      功能预测      粪便移植     
Diversity of microbiota composition of traditional Chinese medicine of Faeces Trogopterori from Shangluo, Shanxi province by sequence of the bacterial 16S rRNA gene
HU Xiao-song1, JIANG Dan1, LI Ting1, XU Zhen1, LIU Jing-ge2, LIU Chun-sheng1
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China;
2. Shang Fang Tang Traditional Chinese Medicine Limited Company, Lanzhou 730030, China
Abstract:
To obtain the microbial composition of traditional Chinese medicine of Faeces Trogopterori, ten samples were collected from the imitate wildness farmland in Shangluo City, Shaanxi Province. In this study, 16S rRNA gene was used as molecular marker to explore the microbiome and the sequences were analyzed by Usearch analysis platform. The COG and KEGG database is used to predict and analyze the function of the flora. A great number of 285 218 high quality clean reads with a length of 400-450 bp were obtained from 10 samples. Bacterial species detected in these samples covered 8 phyla, 25 families, 75 genera and 120 species. The dominant phylum microbial communities in these samples were Firmicutes (87.68% ±2.68%) and the Bacteroidetes (7.62% ±3.74%), all samples showed a high microbial diversity, the predicted functional metagenome was heavily involved in energy metabolism. This study provided that the beneficial bacteria in Faeces Trogopterori may be one of its active ingredients, and no pathogens are detected in the sample.
Key words:    Faeces Trogopterori    flora structure    16sRNA sequencing    functional prediction    fecal transplantation   
收稿日期: 2019-06-28
DOI: 10.16438/j.0513-4870.2019-0517
基金项目: 尚方堂中药有限公司-北京中医药大学横向课题——中药材五灵脂的菌群测定及菌群库的建立(2017110031012434).
通讯作者: 刘敬阁,Tel:86-10-84738624,E-mail:max_liucs@263.net;刘春生,Tel:86-931-8254542,E-mail:ljg@shangfangtang.com
Email: max_liucs@263.net;ljg@shangfangtang.com
相关功能
PDF(1458KB) Free
打印本文
0
作者相关文章
胡小松  在本刊中的所有文章
姜丹  在本刊中的所有文章
李婷  在本刊中的所有文章
许贞  在本刊中的所有文章
刘敬阁  在本刊中的所有文章
刘春生  在本刊中的所有文章

参考文献:
[1] Nanjing University of Chinese Midicine. Great Dictionary of Chinese Medicine (中药大辞典)[M]. Shanghai:Shanghai Science and Technology Press, 2006:515.
[2] Numata A, Takahashi C, Miyamoto T, et al. New triterpenes from a Chinese medicine, Goreishi[J]. Chem Pharm Bull, 1990, 38:942-944.
[3] Yang DM, Su SW, LI X, et al. Study on the active ingredients of Faeces Tro-gopterori[J]. Acta Pharm Sin (药学学报), 1987, 22:223-225.
[4] Jiao Y, Li DY, Liu XQ, et al. Extraction and separation of two flavonoids from Faeces Trogopterori and identification by thin layer chromatography[J]. J Chin Med Mater (中药材), 2009, 32:1039-1041.
[5] Liu JX, Wang YL, Li Y, et al. Experimental study on the effect of Sishen Wan on intestinal flora in rats with diarrhea-type irritable bowel syndrome[J]. Acta Pharm Sin (药学学报), 2019, 54:670-677.
[6] Gao BB, Wang RJ, Peng Y, et al. Effects of a homogeneous polysaccharide from Sijunzi Decoction on human intestinal microbes, including structures and metabolites, in vitro[J]. J Ethnopharmacol, 2018, 6:223-231.
[7] Wu SH, Li HB, Lv N, et al. Interactions between Cannabis Fructus and human gut microflora[J]. Chin Tradit Herb Drugs (中草药), 2019, 50:1189-1197.
[8] Gweon HS, Oliver A, Taylor J, et al. PIPITS:an automated pipeline for analyses of fungal internal transcribed spacer sequences from the Illumina sequencing platform[J]. Methods Ecol Evol, 2015, 6:973-980.
[9] Li WC, Hou QC, Wang YJ, et al. Analysis of the gut microbial diversity of dairy cows during peak lactation by PacBio single-molecule real-time (SMRT) sequencing[J]. Curr Microbiol, 2018, 6:322-330.
[10] Zhao L, Zhang F, Ding X, et al. Gut bacteria selectively promoted by dietary fibers alleviate type 2 diabetes[J]. Science, 2018, 359:1151-1156.
[11] Inagaki A, Sakata T. Dose-dependent stimulatory and inhibitory effects of luminal and serosal n-butyric acid on epithelial cell proliferation of pig distal colonic mucosa[J]. J Nutr Sci Vit, 2005, 51:156-160.
[12] Fukuda S, Toh H, Hase K, et al. Bifidobacteria can protect from enteropa-thogenic infection through production of acetate[J]. Nature, 2012, 469:543-547.
[13] Qiao J, Kwok L, Zhang J, et al. Reduction of Lactobacillus in the milks of cows with subclinical mastitis[J]. Benef Microbes, 2015, 6:485-490.