药学学报, 2019, 54(12): 2340-2344
引用本文:
李书芬, 王静, 孙桂芝, 张涛, 余利岩, 江冰娅, 武临专. 一种检测安丝菌素效价的生物检定方法——木霉平板法[J]. 药学学报, 2019, 54(12): 2340-2344.
LI Shu-fen, WANG Jing, SUN Gui-zhi, ZHANG Tao, YU Li-yan, JIANG Bing-ya, WU Lin-zhuan. Bioassay of ansamitocin by Trichoderma plate[J]. Acta Pharmaceutica Sinica, 2019, 54(12): 2340-2344.

一种检测安丝菌素效价的生物检定方法——木霉平板法
李书芬, 王静, 孙桂芝, 张涛, 余利岩, 江冰娅, 武临专
中国医学科学院、北京协和医学院医药生物技术研究所, 卫健委抗生素生物工程重点实验室, 中国医学科学院药物合成生物学重点实验室, 北京 100050
摘要:
本文报道一种安全高效的安丝菌素生物检定方法。对1株安丝菌素敏感的真菌进行了ITS序列系统进化树分析,确定其属于木霉属,并将其命名为木霉CPCC 400749。采用PDA培养基制备了木霉CPCC 400749检定平板并用于测定安丝菌素效价,测定结果与HPLC分析方法得到的数值基本一致。本文报道的安丝菌素生物检定方法具有安全高效特点,有望用于高通量筛选束丝放线菌安丝菌素高产菌株。
关键词:    安丝菌素      生物检定法      木霉CPCC 400749     
Bioassay of ansamitocin by Trichoderma plate
LI Shu-fen, WANG Jing, SUN Gui-zhi, ZHANG Tao, YU Li-yan, JIANG Bing-ya, WU Lin-zhuan
NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Abstract:
This paper describes a bioassay method for the determination of ansamitocin titers. A fungal strain sensitive to ansamitocin was classified to the genus Trichoderma based on phylogenetic analysis of its ITS sequence, and designated as Trichoderma CPCC 400749. PDA plates of Trichoderma CPCC 400749 were prepared to assay ansamitocin titers of Actinosynnema pretiosum ATCC 31565. The titers were consistent with those determined by HPLC. The bioassay method may have the potential use in high-throughput screening for Actinosynnema pretiosum mutants with improved ansamitocin titers.
Key words:    ansamitocin    bioassay    Trichoderma CPCC 400749   
收稿日期: 2019-08-05
DOI: 10.16438/j.0513-4870.2019-0628
基金项目: “重大新药创制”国家科技重大专项(2018ZX09711001);国家微生物资源平台(NIMR-2018-3).
通讯作者: 武临专,Tel:86-10-63165283,E-mail:wulinzhuan@imb.pumc.edu.cn
Email: wulinzhuan@imb.pumc.edu.cn
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