药学学报, 2020, 55(1): 74-82
引用本文:
庞溢媛, 周玉枝, 宋佳, 杜冠华, 秦雪梅. 基于1H NMR血清和肝脏代谢组学的黄芩叶抗衰老作用研究[J]. 药学学报, 2020, 55(1): 74-82.
PANG Yi-yuan, ZHOU Yu-zhi, SONG Jia, DU Guan-hua, QIN Xue-mei. Anti-aging effect of Scutellaria baicalensis Georgi leaves based on 1H NMR serum and liver metabolomics[J]. Acta Pharmaceutica Sinica, 2020, 55(1): 74-82.

基于1H NMR血清和肝脏代谢组学的黄芩叶抗衰老作用研究
庞溢媛1,2, 周玉枝1, 宋佳1,2, 杜冠华1,3, 秦雪梅1
1. 山西大学中医药现代研究中心, 山西 太原 030006;
2. 山西大学化学化工学院, 山西 太原 030006;
3. 中国医学科学院、北京协和医学院药物研究所, 北京 100050
摘要:
本实验采用D-半乳糖致衰老大鼠模型结合1H NMR血清和肝脏代谢组学,探究黄芩叶醇提物的抗衰老作用以及潜在抗衰老代谢调节机制。所有动物实验均通过山西大学科学研究伦理审查委员会审查。结果表明,黄芩叶醇提物具有抗衰老作用,能够改善衰老大鼠外在表征、自主活动能力和脂质过氧化、糖基化等衰老损伤,并可通过调节血清中谷氨酸、谷氨酰胺等12种差异代谢物和D-谷氨酰胺和D-谷氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢等11个代谢通路,调节肝脏中α-葡萄糖、β-葡萄糖等5种差异代谢物和糖酵解或糖异生、淀粉和蔗糖代谢通路,改善衰老大鼠血清和肝脏代谢异常。
关键词:    黄芩叶      抗衰老      代谢组学      血清      肝脏     
Anti-aging effect of Scutellaria baicalensis Georgi leaves based on 1H NMR serum and liver metabolomics
PANG Yi-yuan1,2, ZHOU Yu-zhi1, SONG Jia1,2, DU Guan-hua1,3, QIN Xue-mei1
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;
2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
3. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Abstract:
In this study a D-galactose-induced aging rat model combined with 1H NMR of serum and liver metabolomics were used to explore the anti-aging effect and the potential metabolic regulatory mechanism of Scutellaria baicalensis Georgi leaves. All procedures involving animal treatment were approved according to the Committee on the Ethics of Animal Experiments of Shanxi University. The results of physical characteristics, an open field test and serum biochemical indexes indicated that Scutellaria baicalensis Georgi leaves had an anti-aging effect that could ameliorate the characteristics of aging rats such as acquired hair loss and slow response, improve the spontaneous activity of aging rats, and decrease lipid peroxidation and glycosylation damage induced by D-galactose. Serum and liver metabolomics further revealed that Scutellaria baicalensis Georgi leaves could decrease serum and liver metabolism disturbances in aging rats, mainly through different metabolites and metabolic pathways. Specifically, 12 differential metabolites including glutamine and glutamate, 11 metabolic pathways including D-glutamine and D-glutamate metabolism and alanine, aspartate and glutamate metabolism in serum were significantly altered after the treatment. Simultaneously, five differential metabolites such as α-glucose and β-glucose, two metabolic pathways that are glycolysis or gluconeogenesis, and starch and sucrose metabolism in the liver were markedly altered.
Key words:    Scutellaria baicalensis Georgi leaf    anti-aging    metabolomics    serum    liver   
收稿日期: 2019-06-17
DOI: 10.16438/j.0513-4870.2019-0377
基金项目: 山西省重点研发计划项目(201703D321023-3).
通讯作者: 周玉枝,Tel:86-351-7019178,E-mail:zhouyuzhi@sxu.edu.cn;秦雪梅,Tel:86-351-7011501,E-mail:qinxm@sxu.edu.cn
Email: zhouyuzhi@sxu.edu.cn;qinxm@sxu.edu.cn
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