药学学报, 2020, 55(1): 106-109
引用本文:
安法梁, 罗俊, 孔令义. 鹧鸪花果实trichilin-type柠檬苦素化学成分研究[J]. 药学学报, 2020, 55(1): 106-109.
AN Fa-liang, LUO Jun, KONG Ling-yi. Trichilin-type limonoids isolated from the fruits of Trichilia connaroides[J]. Acta Pharmaceutica Sinica, 2020, 55(1): 106-109.

鹧鸪花果实trichilin-type柠檬苦素化学成分研究
安法梁, 罗俊, 孔令义
中国药科大学中药学院, 天然药物活性组分与药效国家重点实验室, 江苏省生物活性天然产物研究重点实验室, 江苏 南京 211198
摘要:
通过常压硅胶、凝胶、反相柱色谱和高压反相柱色谱等色谱技术对楝科植物鹧鸪花果实的化学成分进行研究,从鹧鸪花(Trichilia connaroides)果实乙醇提取物中分离获得4个trichilin-type柠檬苦素,包括2个新化合物和2个已知化合物3α-deacetylamoorastatin(3)和mesendanins K(4)。通过综合运用HR-ESI-MS、1H NMR、13C NMR、HSQC、HMBC和ROESY等多种光谱分析方法确定了新化合物12的平面结构和相对构型。通过对分离获得的化合物进行肿瘤细胞毒活性研究,结果表明新化合物12和已知化合物4对宫颈癌细胞(HeLa)具有微弱的细胞毒活性。
关键词:    鹧鸪花      柠檬苦素      细胞毒活性     
Trichilin-type limonoids isolated from the fruits of Trichilia connaroides
AN Fa-liang, LUO Jun, KONG Ling-yi
Jiangsu Key Laboratory of Bioactive Natural Product Research, State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China
Abstract:
Two new trichilin-type limonoids were isolated from the fruits of Trichilia connaroides along with two known limonoids, 3α-deacetylamoorastatin (3) and mesendanins K (4). Their planar structure and relative configuration were elucidated by comprehensive analyses of HR-ESI-MS, 1H NMR, 13C NMR, HSQC, HMBC and ROESY data. An antitumor activity assay showed that compounds 1, 2 and known compound 4 had weak cytotoxicity against a human cervical cancer cell line (HeLa).
Key words:    Trichilia connaroides    limonoids    cytotoxicity   
收稿日期: 2019-09-09
DOI: 10.16438/j.0513-4870.2019-0736
基金项目: 国家自然科学基金资助项目(31470416).
通讯作者: 孔令义,E-mail:cpu_lykong@126.com
Email: cpu_lykong@126.com
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