药学学报, 2020, 55(2): 289-293
引用本文:
吕锦锦, 张靖柯, 张贝贝, 刘娟娟, 李孟, 石静亚, 朱登辉, 杨航, 郑晓珂, 冯卫生. 九蒸九晒熟地黄中的一个新生物碱[J]. 药学学报, 2020, 55(2): 289-293.
L� Jin-jin, ZHANG Jing-ke, ZHANG Bei-bei, LIU Juan-juan, LI Meng, SHI Jing-ya, ZHU Deng-hui, YANG Hang, ZHENG Xiao-ke, FENG Wei-sheng. A new alkaloid from Rehmanniae Radix Preparata[J]. Acta Pharmaceutica Sinica, 2020, 55(2): 289-293.

九蒸九晒熟地黄中的一个新生物碱
吕锦锦1,2, 张靖柯1,2, 张贝贝1,2, 刘娟娟1,2, 李孟1,2, 石静亚1,2, 朱登辉1,2, 杨航1, 郑晓珂1,2, 冯卫生1,2
1. 河南中医药大学药学院, 河南 郑州 450046;
2. 呼吸疾病中医药防治省部共建协同创新中心, 河南 郑州 450046
摘要:
采用多种色谱技术从九蒸九晒熟地黄70%丙酮提取物中分离得到6个生物碱,根据化合物的理化性质及波谱特征对其结构进行鉴定,分别为地黄新碱A(4-{[(5-O-α-D-galactopyranosyloxy)methyl]-1H-pyrrole-2-carbaldehyde-1-yl}butyricacid methyl ester)(1)、baimantuoluoamide B(2)、capparisine C(3)、harman-3-carboxylic acid(4)、(2S)-1-[2-(furan-2-yl)-2-oxoethyl]-5-oxopyrrolidine-2-carboxylate(5)和1-[2-(furan-2-yl)-2-oxoethyl]pyrrolidin-2-one(6)。其中,化合物1为新化合物,化合物26均首次从地黄中分离得到。采用细胞实时监控检测方法(RTCA)探究6个化合物对脂多糖(LPS)诱导的NRK-52e细胞损伤的干预作用,结果表明,化合物13对LPS诱导的NRK-52e细胞损伤均具有保护作用。
关键词:    熟地黄      化学成分      生物碱      NRK-52e细胞      RTCA     
A new alkaloid from Rehmanniae Radix Preparata
L� Jin-jin1,2, ZHANG Jing-ke1,2, ZHANG Bei-bei1,2, LIU Juan-juan1,2, LI Meng1,2, SHI Jing-ya1,2, ZHU Deng-hui1,2, YANG Hang1, ZHENG Xiao-ke1,2, FENG Wei-sheng1,2
1. School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China;
2. Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Disease Diagnosis by Henan and Education Ministry of P. R. China, Zhengzhou 450046, China
Abstract:
The chemical constituents of Rehmanniae Radix Preparata were prepared according to the traditional method of "jiu zheng jiu shai" and investigated using multiple chromatographic methods. Six alkaloids were isolated and their structures were elucidated from spectral data and physicochemical properties, as follows:rehmanniae alkaloid A (4-{[(5-O-á-D-galactopyranosyloxy)methyl]-1H-pyrrole-2-carbaldehyde-1-yl}butyric acidmethyl ester) (1), baimantuoluoamide B (2), capparisine C (3), harman-3-carboxylic acid (4), (2S)-1-[2-(furan-2-yl)-2-oxoethyl]-5-oxopyrrolidine-2-carboxylate (5), and 1-[2-(furan-2-yl)-2-oxoethyl]pyrrolidin-2-one (6). Among them, compound 1 is a new alkaloid. Compounds 2-6 were newly isolated from Rehmannia glutinosa Libosch. The effect of compounds 1-6 on NRK-52e cell injury induced by LPS was investigated. The results show that compounds 1-3 exhibit protective effects against LPS-induced damage to NRK-52e cells.
Key words:    Rehmanniae Radix Preparata    chemical compositions    alkaloids    NRK-52e cells    RTCA   
收稿日期: 2019-09-04
DOI: 10.16438/j.0513-4870.2019-0716
基金项目: 国家重点研发计划中医药现代化研究重点专项“地黄特色中药材产业链关键技术研究”(2017YFC1702800).
通讯作者: 冯卫生,Tel/Fax:86-371-60190296;E-mail:dffwsh@hactcm.edu.cn
Email: dffwsh@hactcm.edu.cn
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