药学学报, 2020, 55(2): 298-304
引用本文:
江虹, 庞向东, 胡影. 虎红探针测定盐酸美西律—基于双波长共振光散射技术[J]. 药学学报, 2020, 55(2): 298-304.
JIANG Hong, PANG Xiang-dong, HU Ying. Determination of mexiletine hydrochloride by rose Bengal probe using dual-wavelength resonance light scattering technology[J]. Acta Pharmaceutica Sinica, 2020, 55(2): 298-304.

虎红探针测定盐酸美西律—基于双波长共振光散射技术
江虹, 庞向东, 胡影
长江师范学院化学化工学院, 武陵山片区绿色发展协同创新中心, 重庆408100
摘要:
探讨了虎红与盐酸美西律在溴代十六烷基吡啶存在下相互作用的共振光散射(RLS)光谱特征,建立了测定药物中盐酸美西律的双波长共振光散射(DWO-RLS)新方法。在弱酸性溶液中,虎红与盐酸美西律及溴代十六烷基吡啶相互作用生成红色三元缔合物,使共振光散射信号显著增强,并在372和596 nm波长处产生2个强的特征散射峰。在这二波长处,盐酸美西律在0.004~0.65 mg·L-1内的质量浓度与共振光散射增强强度(ΔIRLS)呈线性关系,检出限分别为0.003 2 mg·L-1(372 nm)和0.003 8mg·L-1(596 nm)。当用DWO-RLS技术测定时,其检出限更低,仅为0.001 8 mg·L-1,将DWO-RLS法用于市售盐酸美西律药片中盐酸美西律的测定,回收率为98.5%~103%,相对标准偏差为2.0%~2.7%。
关键词:    盐酸美西律      溴代十六烷基吡啶      虎红      双波长      共振光散射     
Determination of mexiletine hydrochloride by rose Bengal probe using dual-wavelength resonance light scattering technology
JIANG Hong, PANG Xiang-dong, HU Ying
College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Green Development in Wuling Mountain Areas, Yangtze Normal University, Chongqing 408100, China
Abstract:
The resonance light scattering (RLS) spectral characteristics of the interaction between rose Bengal and mexiletine hydrochloride in the presence of cetylpyridinium bromide were investigated. A dual-wavelength resonance light scattering (DWO-RLS) method for the determination of mexiletine hydrochloride in drugs was established. In a weakly acidic solution, rose Bengal interacts with mexiletine hydrochloride and cetylpyridinium bromide to form a red ternary ion association complex, which led to a significantly enhanced resonance light scattering signal and produced two strong characteristic scattering peaks at 372 nm and 596 nm. In these two wavelengths the mass concentration of mexiletine hydrochloride was in the range of 0.004 to 0.65 mg·L-1 and had a good linear relationship with the resonance light scattering enhancement intensity (ΔIRLS), with detection limits of 0.003 2 mg·L-1 (372 nm) and 0.003 8 mg·L-1 (596 nm), respectively. When measured by the dual-wavelength resonance light scattering (DWO-RLS) technique, the detection limit was lower, only 0.001 8 mg·L-1. When the DWO-RLS method was applied to the determination of mexiletine hydrochloride in commercially available mexiletine hydrochloride tablets, and the recovery was 98.5%-103%, and the relative standard deviation was 2.0%-2.7%.
Key words:    mexiletine hydrochloride    cetylpyridinium bromide    rose Bengal    dual-wavelength    resonance light scattering   
收稿日期: 2019-07-07
DOI: 10.16438/j.0513-4870.2019-0538
基金项目: 重庆市教委科技基金资助项目(KJ1401226);长江师范学院科技基金资助项目(2018CXX117).
通讯作者: 江虹,Tel:86-23-72790016,E-mail:jianghongch@163.com
Email: jianghongch@163.com
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参考文献:
[1] Shibuya K, Misawa S, Kimura H, et al. A single blind randomized controlled clinical trial of mexiletine in amyotrophic lateral sclerosis:efficacy and safety of sodium channel blocker phase Ⅱ trial[J]. Amyotro Lat Scl Frontotemp Deg, 2015, 16:353-358.
[2] Zheng B, Zhou XH. Effect analysis of amiodarone combined with mexiletine hydrochloride in treatment of ICU high risk tachyarrhythmia[J]. China Mod Med (中国当代医药), 2014, 21:63-65.
[3] Zeng Q, Zhang Y, Li YH. The combination of mexiletine hydrochloride and metoprolol tartrate tablets in treating premature ventricular beat caused by left ventricular false chordae tendineae[J]. J Pract Electrocardiol, 2015, 24:204-205.
[4] Yu L, Hong Y, Li L, et al. Enantioselective drug-protein interaction between mexiletine and plasma protein[J]. J Pharm Pharmacol, 2012, 64:792-801.
[5] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (中华人民共和国药典)[M]. 2015 Ed. Part Ⅱ. Beijing:China Medical Science Press, 2015:1042.
[6] Jin WH, Chen H, Zhang M, et al. Preparationand quality control of mexiletine hydrochloride suppository[J]. Pharm J Chin PLA (解放军药学学报), 2017, 33:247-249.
[7] Liu HL, Han XJ, Yan K. Determination of mexiletine hydrochloride tablets by HPLC[J]. China Pharm (中国药业), 2012, 21:20-21.
[8] Han XJ, Zhang YH, Pang WZ. Determination of mexiletine hydrochloride in tablets by HPLC[J]. West China J Pharm Sci (华西药学杂志), 2011, 26:77-78.
[9] Dragan MM, Zoran BT. Development and application of a validated HPLC method for the analysis of dissolution samples of mexiletine hydrochloride capsules[J]. J Serb Chem Soc, 2010, 75:975-985.
[10] Zhang H, Yu C, Liu GY, et al. HPLC-fluorescent spectrometric determination of serum mexiletine concentration after derivatization with fluram[J]. Acta Pharm Sin (药学学报), 2003, 38:215-217.
[11] Zhang DD, Sun MJ, Ouyang HZ, et al. Pharmacokinetics of mexiletine hydrochloride tablets in rats[J]. Drugs Clin (现代药物与临床), 2017, 32:2309-2312.
[12] Yang M, Cui Y, Wu W. Content determination of mexiletine hydrochloride tablets by HPLC-MS/MS[J]. China Pharm (中国药业), 2017, 26:35-37.
[13] Han XJ, Du ZH, Wang M. Determination of mexiletine hydrochlorideand 2,6-dimethylphenol by GC[J]. China Pract Med (中国实用医药), 2010, 5:1-2.
[14] Sun Y, Peng HD, Wei HY, et al. Determination of mexiletine hydrochloride in tablets by microchip capillary electrophoresis with laser induced fluorescence detection[J]. Chin J Anal Lab (分析实验室), 2012, 31:13-17.
[15] Gao XJ, Zhang PP, Zheng QX, et al. Determination of mexiletine hydrochloride in mexiletine hydrochloride tablets by fluorescence spectrophotometry[J]. Chem Res Appl (化学研究与应用), 2017, 29:94-98.
[16] Zhang LB, Wu H, Du LM. Determination of mexiletine hydrochloride by derivative-ionic liquid extraction spectrophotometry[J]. J Anal Sci (分析科学学报), 2012, 28:173-176.
[17] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (中华人民共和国药典)[M]. 2015 Ed. Part Ⅱ. Beijing:China Medical Science Press, 2015:1043.
[18] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (中华人民共和国药典)[M]. 2005 Ed. Part Ⅱ. Beijing:Chemical Industry Press, 2005:551.
[19] He SH, Zeng QR, Chen YZ, et al. Determination of aztreonam in drug and urine sample by high sensitive resonance light scattering technique[J]. J Anal Sci (分析科学学报), 2019, 35:393-396.
[20] Jiang H, Pang XD, Wu XY, et al. Studies on detecting carbamazepine residues in food using high sensitive resonant light scattering technique[J]. Food Ferment Ind (食品与发酵工业), 2019, 45:206-210.
[21] Pang XD, Jiang H, He TT, et al. Determination of trace zinc (Ⅱ) in landfill leachate by resonance light scattering method with azo chlorination Ⅲ-hexadecylpyridinium bromide after sulfhydryl cotton separation and preconcentraction[J]. Environ Monit China (中国环境监测), 2018, 34:127-131.
[22] Jiang H, Wang F, Wu ZZ, et al. Determination of Cu in food by dual-wavelength rayleigh light scattering technique[J]. Food Sci (食品科学), 2018, 39:323-327.