药学学报, 2020, 55(12): 2869-2882
引用本文:
江翠平, 王媛, 肖海燕, 赵海越, 刘强. 非病毒型载体聚乙烯亚胺在基因递送应用中的研究进展[J]. 药学学报, 2020, 55(12): 2869-2882.
JIANG Cui-ping, WANG Yuan, XIAO Hai-yan, ZHAO Hai-yue, LIU Qiang. Recent progress of non-viral vector polyethylenimine in the application of gene delivery[J]. Acta Pharmaceutica Sinica, 2020, 55(12): 2869-2882.

非病毒型载体聚乙烯亚胺在基因递送应用中的研究进展
江翠平, 王媛, 肖海燕, 赵海越, 刘强
南方医科大学中医药学院, 广东省中药制剂重点实验室, 广东省中药制剂技术工程实验室, 广东 广州 510515
摘要:
非病毒型基因载体因具有低毒性、低免疫原性及易制备等诸多优点,近年来成为制备高效低毒型基因递送载体的研究热点。聚乙烯亚胺(PEI)作为典型的非病毒型基因载体以其较好的基因压缩能力及溶酶体逃逸能力引起了广泛关注。本文对基于PEI类基因载体的体内递送过程及所面临的问题(如生物相容性低、细胞毒性、缺乏特异靶向性和目的基因释放不足等)进行了归纳与分析,并总结了相应的改进方案。此外,对基于PEI的智能响应型基因载体(内源性刺激响应型、外源性刺激响应型)的研究进展进行了综述,为开发出更加安全高效的非病毒型基因载体提供参考。
关键词:    基因载体      非病毒类载体      基因传递系统      聚乙烯亚胺      靶向治疗     
Recent progress of non-viral vector polyethylenimine in the application of gene delivery
JIANG Cui-ping, WANG Yuan, XIAO Hai-yan, ZHAO Hai-yue, LIU Qiang
Guangdong Provincial Engineering Laboratory of Chinese Medicine Preparation Technology, Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, School of Traditional Chinese medicine, Southern Medical University, Guangzhou 510515, China
Abstract:
In recent years, non-viral gene vectors have attracted great attention for efficient gene delivery due to the advantages, including low toxicity, low immunogenicity and simple preparation. Polyethylenimine (PEI) is one of the typical non-viral gene carriers that have been widely utilized for gene delivery owing to its superior capabilities in gene compression and buffering capacity. This article discusses the processes of gene delivery and the barriers of PEI-based carrier during the gene delivery, such as low biocompatibility, cytotoxicity, lack of specific targeting and insufficient gene release, etc. Therefore, we summarize the multiple approaches for the modifications of PEI in terms of improved biocompatibility, degradability, specific targeting and buffering capacity. Furthermore, we also review on the recent impressive progresses of smart stimuli-responsive PEI carriers, including endogenous stimuli (pH, reactive oxygen species, glutathione, biomolecular, etc), exogenous stimuli (light, temperature, magnetic field, etc) and dual-responsive strategies, which might provide guidance for the development of more efficient and safer non-viral gene vectors.
Key words:    gene vector    non-viral vector    gene delivery system    polyethylenimine    targeting therapy   
收稿日期: 2020-05-12
DOI: 10.16438/j.0513-4870.2020-0740
基金项目: 国家自然科学基金资助项目(81874346);广东省中医药局科研课题(20200430105634).
通讯作者: 刘强,Tel/Fax:86-20-61648263,E-mail:cuipingjiangcpu@163.com
Email: cuipingjiangcpu@163.com
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