药学学报, 2020, 55(12): 2951-2954
引用本文:
何晨, 曹彦刚, 齐曼, 张贝贝, 任英杰, 刘晏灵, 王梦娜, 郑晓珂, 冯卫生. 皂角刺中的一个新木脂素[J]. 药学学报, 2020, 55(12): 2951-2954.
HE Chen, CAO Yan-gang, QI Man, ZHANG Bei-bei, REN Ying-jie, LIU Yan-ling, WANG Meng-na, ZHENG Xiao-ke, FENG Wei-sheng. A new lignan from Gleditsiae spina[J]. Acta Pharmaceutica Sinica, 2020, 55(12): 2951-2954.

皂角刺中的一个新木脂素
何晨1,2, 曹彦刚1,2, 齐曼1,2, 张贝贝1,2, 任英杰1,2, 刘晏灵1,2, 王梦娜1,2, 郑晓珂1,2, 冯卫生1,2
1. 河南中医药大学药学院, 河南 郑州 450046;
2. 河南省中药开发工程技术研究中心, 河南 郑州 450046
摘要:
利用MCI gel CHP-20、ODS、Sephadex LH-20、硅胶及半制备高效液相等多种色谱技术,从皂角刺乙酸乙酯部位分离得到7个木脂素类化合物,并根据波谱数据分析鉴定其结构,分别为(7R,8S,7'E,7''S,8''R)-buddlenol P(1)、(+)-丁香脂素(2)、(+)-异落叶松脂素(3)、(7S,8R)-cedrusin(4)、(7S,8R)-4,9,9'-三羟基-3,3'-二甲氧基-7,8-二氢苯骈呋喃-1'-丙基新木脂素(5)、3',4-O-dimethylcedrusin(6)和蛇菰宁(7)。其中化合物1为新化合物,命名为(7R,8S,7'E,7''S,8''R)-buddlenol P,化合物27为首次从皂荚属中分离得到。采用MTT法探究化合物27对脂多糖(LPS)诱导的大鼠肾小管上皮细胞损伤的干预作用,化合物237对LPS诱导的NRK-52e细胞损伤具有保护作用。
关键词:    皂荚属      皂角刺      木脂素类      (7R,8S,7'E,7''S,8''R)-buddlenol P     
A new lignan from Gleditsiae spina
HE Chen1,2, CAO Yan-gang1,2, QI Man1,2, ZHANG Bei-bei1,2, REN Ying-jie1,2, LIU Yan-ling1,2, WANG Meng-na1,2, ZHENG Xiao-ke1,2, FENG Wei-sheng1,2
1. School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China;
2. The Engineering and Technology Center for Chinese Medicine Development of Henan Province China, Zhengzhou 450046, China
Abstract:
The chemical constituents from ethyl acetate extract of Gleditsiae spina were isolated and purified by various chromatographic methods such as MCI gel CHP-20, ODS, Sephadex LH-20, silica gel and semi-preparative HPLC. Seven lignans were isolated and identified by spectroscopic data analyses as (7R,8S,7'E,7''S,8''R)-buddlenol P (1), (+)-syringaresinol (2), (+)-isolariciresinol (3), (7S,8R)-cedrusin (4), (7S,8R)-4,9,9'-trihydroxy-3,3'-dimethoxy-7,8-dihydrobenzofuran-1'-propylneolignan (5), 3',4-O-dimethylcedrusin (6), balanophonin (7). Among them, compound 1 is a new lignan, compounds 2-7 are isolated from the Gleditsia L. for the first time. MTT method was used to investigate the effect of compounds 2-7 on LPS-induced injury of NRK-52e cells. As a result, compounds 2, 3 and 7 exhibit protective effects against LPS-induced damage to NRK-52e cells.
Key words:    Gleditsia L.    Gleditsiae spina    lignans    (7R,8S,7'E,7''S,8''R)-buddlenol P   
收稿日期: 2020-07-27
DOI: 10.16438/j.0513-4870.2020-1241
基金项目: 中央引导地方科技重大发展专项:河南道地大宗药材种质评价及集约化种植与示范(豫财科[2016]149号).
通讯作者: 冯卫生,Tel/Fax:86-370-60190296,E-mail:fwsh@hactcm.edu.cn
Email: fwsh@hactcm.edu.cn
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参考文献:
[1] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (中华人民共和国药典一部)[S]. Part 1. Beijing:China Medical Science Press, 2015:177.
[2] Long L, Geng GX, Li QW. Effect of Gleditsia sinensis stings on growth inhibition and expression of PCNA and p53 in mice bearing uterine cervical carcinoma (U14)[J]. China J Chin Mater Med (中国中药杂志), 2006, 31:150-152.
[3] Zhao B, Li HR, Du GJ. Experimental study on the anti-tumor activities of Spina Gleditsiae combined with Cortex cinnamomi[J]. J Henan Univ (Med Sci) (河南大学学报:医学版), 2013, 32:88-92.
[4] Zhang L, Wang HH, Xu RH, et al. Study on protective effect of extracts from Gleditsiae Spina on PC-12 cells injury induced by corticosterone[J]. Chin Med Mat (中药材), 2020, 43:1477-1481.
[5] Qi M, Zheng XK, Cao YG, et al. Chemical constituents from ethyl acetate extract of Gleditsiae Spina[J]. Chin Tradit Herb Drugs (中草药), 2018, 49:5510-5515.
[6] Yang XW, Zhao PJ, Ma YL, et al. Mixed lignan-neolignans from Tarenna attenuata[J]. J Nat Prod, 2007, 70:521-525.
[7] Wang LY, Chen MH, Wu J, et al. Bioactive glycosides from the twigs of Litsea cubeba[J]. J Nat Prod, 2017, 80:1808-1818.
[8] Gan ML, Zhang YL, Lin S, et al. Glycosides from the root of Iodes cirrhosa[J]. J Nat Prod, 2008, 71:647-654.
[9] Ji HL, Sai M, Zhou SY, et al. A new dihydroflavone from Mirabilis himalaica[J]. Acta Pharm Sin (药学学报), 2018, 53:97-99.
[10] Zhang J, Sun GB, Lei QF, et al. Chemical constituents of Aconiti Lateralis Radix Praeparata[J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志), 2014, 20:108-111.
[11] Kim TH, Ito H, Hayashi K, et al. Aromatic constituents from the heartwood of Santalum album L.[J]. Chem Pharm Bull, 2005, 53:641-644.
[12] Feng WS, Chen H, Zheng XK. Chemical constituents of Selaginella sinensis[J]. Chin Tradit Herb Drugs (中草药), 2008, 39:654-656.
[13] Feng WS, Bi YF, Zheng XK, et al. Studies on the lignan chemical constituents from pine needles of Pinus massoniana Lamb.[J]. Acta Pharm Sin (药学学报), 2003, 38:199-202.
[14] Luc P, Tess DB, Alex DG, et al. NMR study of some dihydrobenzofuran lignans[J]. Magn Reson Chem, 1993, 31:692-693.
[15] Xu MM, Duan YH, Xiao HH, et al. Lignans isolated from stems of Sambucus williamsii and their proliferation effects on UMR 106 cells[J]. China J Chin Mater Med (中国中药杂志), 2014, 39:2684-2688.