药学学报, 2020, 55(12): 2968-2975
引用本文:
石丽霞, 李科, 秦雪梅, 李震宇, 崔连杰, 李树颖, 曹宇欣, 王盛金. 基于糖特征图谱的不同柴胡的品种鉴别[J]. 药学学报, 2020, 55(12): 2968-2975.
SHI Li-xia, LI Ke, QIN Xue-mei, LI Zhen-yu, CUI Lian-jie, LI Shu-ying, CAO Yu-xin, WANG Sheng-jin. Identification of different Bupleurum varieties based on carbohydrate-specific chromatograms[J]. Acta Pharmaceutica Sinica, 2020, 55(12): 2968-2975.

基于糖特征图谱的不同柴胡的品种鉴别
石丽霞1,2, 李科1,2,3, 秦雪梅1,2, 李震宇1,2, 崔连杰1,2, 李树颖1,2, 曹宇欣1,2, 王盛金4
1. 山西大学中医药现代研究中心, 山西 太原 030006;
2. 山西大学, 化学生物学与分子工程教育部重点实验室, 山西 太原 030006;
3. 中国科学院过程工程研究所, 北京 100190;
4. 山西省晋城市陵川县农业综合开发中心, 山西 晋城 048300
摘要:
通过建立柴胡多糖和单糖特征图谱,找出不同柴胡的糖谱专属性差异为不同品种柴胡的鉴别提供依据。文中采用高效液相色谱仪(HPLC)-蒸发光检测器(ELSD)建立柴胡总多糖特征图谱,多糖经衍生化后通过紫外检测器(UV)建立单糖特征图谱,利用SIMCA软件和SPSS软件对数据进行分析以区分不同品种柴胡。结果显示三岛柴胡多糖的得率最高,而北柴胡多糖的得率最低,多糖谱图显示不同柴胡中的多糖分子质量有区别,而且各自占总峰面积的百分比也不一样。四种柴胡多糖都是由甘露糖、葡萄糖醛酸、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖组成的,但是他们具有不同的单糖物质的量比,北柴胡、红柴胡、三岛柴胡和藏柴胡中葡萄糖与阿拉伯糖的比值依次是:3.0~4.5、5.5~7.0、12.0~17.0和9.0~12.0。本研究采用糖谱技术为不同柴胡的鉴别提供了新方法。
关键词:    柴胡      多糖      单糖      特征图谱      品种鉴别     
Identification of different Bupleurum varieties based on carbohydrate-specific chromatograms
SHI Li-xia1,2, LI Ke1,2,3, QIN Xue-mei1,2, LI Zhen-yu1,2, CUI Lian-jie1,2, LI Shu-ying1,2, CAO Yu-xin1,2, WANG Sheng-jin4
1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China;
2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China;
3. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
4. Agricultural Comprehensive Development Center of Lingchuan County of Jincheng City of Shanxi Province, Jincheng 048300, China
Abstract:
Characterization of the polysaccharides and monosaccharides of Bupleurum chinense was undertaken to identify differences in the Bupleurum chinense's sugar profiles, so as to provide a basis for the identification of different varieties. High performance liquid chromatography (HPLC) was used to generate chromatograms of the total polysaccharides of Bupleurum using an Evaporation Light Detector (ELSD), and a monosaccharide chromatogram was generated using a UV-detector (UV) following polysaccharide derivatization. The data were analyzed using SIMCA software and SPSS software to distinguish different varieties of Bupleurum. The results show that the yield of polysaccharides from Bupleurum falcatum is the highest, while the yield of polysaccharides from Bupleurum chinense is the lowest. The polysaccharide spectrum shows that the molecular weights of the polysaccharides in different Bupleurum differ, and their percentages of the total peak area are also different. The four Bupleurum polysaccharides are composed of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose, and arabinose, but differ in length. The ratio of glucose to arabinose in Bupleurum chinense, Bupleurum scorzonerifolium, Bupleurum falcatum and Bupleurum marginatum var. stenophyllum is: 3.0-4.0, 5.5-7.0, 12.0-17.0, 9.0-12.0. In this study, a sugar profile technique was developed to provide a new method for the identification of different varieties of Bupleurum.
Key words:    Bupleurum    polysaccharide    monosaccharide    characteristic spectrum    variety identification   
收稿日期: 2020-05-24
DOI: 10.16438/j.0513-4870.2019-1054
基金项目: 国家自然科学基金资助项目(81872962);国家重点研发计划(2019YFC1710800);山西省重点研发计划重点项目(201603D311101);山西省优秀人才科技创新项目(201605D211030,201705D211020).
通讯作者: 李科,Tel/Fax:86-351-7019297,E-mail:like@sxu.edu.cn
Email: like@sxu.edu.cn
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