药学学报, 2021, 56(2): 553-556
引用本文:
夏桂阳, 肖标兵, 王玲燕, 方冬杰, 夏欢, 林生. 醋制延胡索中一个新苄基异喹啉二聚体生物碱[J]. 药学学报, 2021, 56(2): 553-556.
XIA Gui-yang, XIAO Biao-bing, WANG Ling-yan, FANG Dong-jie, XIA Huan, LIN Sheng. A new dimeric benzylisoquinoline alkaloid from Corydalis yanhusuo [J]. Acta Pharmaceutica Sinica, 2021, 56(2): 553-556.

醋制延胡索中一个新苄基异喹啉二聚体生物碱
夏桂阳1, 肖标兵2, 王玲燕2, 方冬杰2, 夏欢1, 林生1
1. 北京中医药大学东直门医院, 中医内科学教育部和北京市重点实验室, 北京 100700;
2. 中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050
摘要:
运用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、反相中压柱色谱、半制备高效液相色谱等分离纯化技术,从醋制延胡索(Corydalis yanhusuo W.T.Wang)水煎液乙酸乙酯萃取部位分离获得1个新的C-4~C-7'位连接的苄基异喹啉类二聚体生物碱,通过UV、IR、ESI-MS、HR-ESI-MS、1D NMR和2D NMR等多种波谱分析方法确定其结构,并命名为(±)-bicoryanhunine B (1)。化合物1对PD-1/PD-L1结合有一定抑制活性,IC50为7.80 ±0.49 μmol·L-1;此外,化合物1还可显著抑制脂多糖(LPS)诱导小鼠巨噬细胞RAW 264.7释放NO,IC50值为4.83 ±2.21 μmol·L-1
关键词:    延胡索      二聚体生物碱      PD-1/PD-L1      抗炎     
A new dimeric benzylisoquinoline alkaloid from Corydalis yanhusuo
XIA Gui-yang1, XIAO Biao-bing2, WANG Ling-yan2, FANG Dong-jie2, XIA Huan1, LIN Sheng1
1. Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;
2. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Abstract:
(±)-Bicoryanhunine B (1), a new dimeric benzylisoquinoline alkaloid was isolated from the dried tubers of Corydalis yanhusuo by various chromatographic methods, including silica gel, Sephadex LH-20, reverse phase C18, and semi-preparative HPLC. Its structure was determined by spectroscopic methods, including UV, IR, ESI-MS, HR-ESI-MS and 1D/2D NMR. (±)-Bicoryanhunine B (1) was a moderate PD-1/PD-L1 interaction inhibitor with an IC50value of 7.80 ±0.49 μmol·L-1. In addition, 1 exhibited potent inhibitory activities against LPS-induced NO production in RAW 264.7 macrophages with an IC50value of 4.83 ±2.21 μmol·L-1.
Key words:    Corydalis yanhusuo    dimeric alkaloid    PD-1/PD-L1    anti-inflammatory   
收稿日期: 2020-09-29
DOI: 10.16438/j.0513-4870.2020-1564
基金项目: 北京市自然科学基金杰出青年基金项目(JQ18026);国家自然科学基金面上项目(82073978).
通讯作者: 林生,Tel:86-10-84013404,E-mail:lsznn@126.com
Email: lsznn@126.com
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