药学学报, 2021, 56(3): 865-871
引用本文:
麻培培, 李祺, 欧歌, 张圆圆, 李倩, 杜丽娜. 5-氨基酮戊酸立方液晶联合光针治疗基底细胞癌的研究[J]. 药学学报, 2021, 56(3): 865-871.
MA Pei-pei, LI Qi, OU Ge, ZHANG Yuan-yuan, LI Qian, DU Li-na. The combined therapeutic effects of 5-aminolevulinic acid cubic liquid crystal and light needles for basal cell cancer[J]. Acta Pharmaceutica Sinica, 2021, 56(3): 865-871.

5-氨基酮戊酸立方液晶联合光针治疗基底细胞癌的研究
麻培培1,2, 李祺1,2, 欧歌1, 张圆圆1,2, 李倩1,2, 杜丽娜1,2
1. 军事科学院军事医学研究院辐射医学研究所, 北京 100850;
2. 山东中医药大学药学院, 山东 济南 250355
摘要:
本文评价了局部光动力学疗法与二氧化碳点阵激光-“光针”联合用于治疗基底细胞癌(basal cellcarcinoma,BCC)的药效。以单硬脂酸甘油酯(glyceryl monostearate,GMO)为基质制备了5-氨基酮戊酸(5-aminolevulinic acid,5-ALA)立方液晶,评价了其体外细胞毒性。动物实验获得军事科学院军事医学研究院伦理委员会批准后,以裸鼠死亡率、肿瘤体积和肿瘤转移率为指标评价了5-ALA立方液晶与不同能量光针联合治疗BCC的效果。结果表明,5-ALA立方液晶呈各向同性,为双菱性晶格结构(PN3M)。5-ALA立方液晶联合光针细胞毒性明显大于单独使用5-ALA或光针。5-ALA立方液晶与高能量光针联合应用可防止BCC转移;与低能量光针联用可显著促进药物渗透,抑制BCC生长。综上,5-ALA立方液晶联合光针可有效治疗BCC、防止其转移,为临床治疗提供一种新选择。
关键词:    5-氨基酮戊酸      立方液晶      光针      基底细胞癌      光动力学     
The combined therapeutic effects of 5-aminolevulinic acid cubic liquid crystal and light needles for basal cell cancer
MA Pei-pei1,2, LI Qi1,2, OU Ge1, ZHANG Yuan-yuan1,2, LI Qian1,2, DU Li-na1,2
1. Beijing Institute of Radiation Medicine, Beijing 100850, China;
2. Department of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Abstract:
The article was to study the effect of local photodynamics therapy combined with carbon dioxide lattice laser-"light needles" for the treatment of basal cell carcinoma (BCC). 5-Aminolevulinic acid (5-ALA) cubic liquid crystal using glyceryl monostearate (GMO) as the substrate was prepared. The cytotoxicity of 5-ALA cubic liquid crystal combined with light needles in vitro were evaluated. The pharmacodynamics study of 5-ALA cubic liquid crystal combined with light needles of high or low energy for BCC was carried out based on the pathological changes, tumor volume, vascular endothelial growth factor (VEGF) expression and the recurrence rate, which has been approved by the Ethics Committee of Beijing Institute of Radiation Medicine. The cubic liquid crystal was isotropic with the lattice of PN3M. The cytotoxicity of 5-ALA cubic liquid crystal combined with light needles was much higher than that of 5-ALA or light needles alone. Compared with light needles or photodynamic therapy alone, 5-ALA cubic liquid crystal combined with light needles of high energy could prevent the BCC metastasis and of low energy could inhibit BCC growth. It demonstrated the obvious therapeutical effects for BCC. 5-ALA cubic liquid crystal combined with light needles can effectively treat BCC, which provides a new choice for clinical BCC treatment.
Key words:    5-aminolevulinic acid    cubic liquid crystal    light needle    basal cellcarcinoma    photodynamics   
收稿日期: 2020-10-24
DOI: 10.16438/j.0513-4870.2020-1657
基金项目: 北京自然科学基金面上项目(7202147).
通讯作者: 杜丽娜,Tel:86-10-66930216,E-mail:dulina@188.com
Email: dulina@188.com
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