药学学报, 2021, 56(3): 872-878
引用本文:
贺元, 慕祺瑞, 白贞芳, 姜丹, 任广喜, 刘春生. 基于高通量测序技术的濒危植物黄瑞香Daphne giraldii Nitsche根部内生真菌多样性研究[J]. 药学学报, 2021, 56(3): 872-878.
HE Yuan, MU Qi-rui, BAI Zhen-fang, JIANG Dan, REN Guang-xi, LIU Chun-sheng. The diversity of endophytic fungi in endangered medicinal material Daphnes Cortex as determined by high-throughput sequencing technology[J]. Acta Pharmaceutica Sinica, 2021, 56(3): 872-878.

基于高通量测序技术的濒危植物黄瑞香Daphne giraldii Nitsche根部内生真菌多样性研究
贺元, 慕祺瑞, 白贞芳, 姜丹, 任广喜, 刘春生
北京中医药大学中药学院, 北京 102488
摘要:
通过研究濒危植物黄瑞香根部真菌种类的多样性,为濒危植物黄瑞香真菌库的建立及筛选促进黄瑞香生长和提高其活性成分祖师麻甲素含量的内生真菌提供科学依据。以甘肃、山西两省的9份野生黄瑞香植株为研究对象,利用Illumina高通量测序技术对其测序并进行生物信息学分析,共获得632 766条有效序列,包括348个OTU,4门,20纲,48目,108科,154属,208种,丰度占比前3的真菌属之和能够占到总丰度的65%以上,最高可以达到98.4%,链格孢属(Altemaria)、茎点霉属(Phoma)为黄瑞香的核心菌属,链格孢属为优势属;黄瑞香根部内生真菌多样性丰富,不同产区内生真菌多样性依次为:甘谷县 > 五台县 > 宕昌县。
关键词:    高通量测序      黄瑞香      内生真菌      多样性      濒危植物     
The diversity of endophytic fungi in endangered medicinal material Daphnes Cortex as determined by high-throughput sequencing technology
HE Yuan, MU Qi-rui, BAI Zhen-fang, JIANG Dan, REN Guang-xi, LIU Chun-sheng
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China
Abstract:
In order to provide a scientific basis for the establishment of a Daphnes Cortex medicinal material fungus library and the screening of endophytic fungi that promote the growth of Daphnes Cortex and increase the content of daphnetin, we used Illumina high-throughput testing technology to analyze 9 Daphnes Cortex samples from Gansu and Shanxi provinces. A total of 632 766 valid sequences were obtained, including 348 OTUs, 4 phyla, 20 classes, 48 orders, 108 families, 154 genera, and 208 species. The sum of the first 3 fungal genera account for more than 65% of the total abundance, with the highest reaching 98.4%. Alternaria and Phoma are the main genuses of Daphne giraldii Nitsche, and Altemaria is the dominant genus. The endophytic fungi community of Daphnes Cortex is rich in diversity, and the order of fungal diversity in different producing areas is:Gangu County > Wutai County > Tanchang County.
Key words:    high-throughput sequencing    Daphnes Cortex    endophytic fungi    diversity    endangered plant   
收稿日期: 2020-10-19
DOI: 10.16438/j.0513-4870.2020-1627
基金项目: 2019年北京市双一流人才培养(北京市教学名师奖)项目(1000041510139);北京中医药大刘春生教学名师工作坊项目(1000062420397).
通讯作者: 刘春生,E-mail:Max_liucs@263.net
Email: Max_liucs@263.net
相关功能
PDF(850KB) Free
打印本文
0
作者相关文章
贺元  在本刊中的所有文章
慕祺瑞  在本刊中的所有文章
白贞芳  在本刊中的所有文章
姜丹  在本刊中的所有文章
任广喜  在本刊中的所有文章
刘春生  在本刊中的所有文章

参考文献:
[1] Institute of Traditional Chinese Medicine, Shaanxi Branch of Chinese Academy of Medical Sciences.Shaanxi Journal of Traditional Chinese Medicine (陕西中药志)[M]. Xi'an:Shaanxi People's Publishing House, 1961:342.
[2] Geng L, Yang XH, Li ST, et al. Resource situation investigation and analysis about endangered Chinese herbal medicine Daphnes Cortex[J]. China J Chin Mater Med (中国中药杂志), 2016, 41:3323-3328.
[3] Mu QR, Jiang D, He Y, et al. Correlation between chemical composition, ecological factors and soil factors of Chinese herbal medicine Daphnes Cortex[J]. China J Chin Mater Med (中国中药杂志), 2020, 45:1059-1063.
[4] Herre EA, Mejia LC, Kyllo DA, et al. Ecological implications of anti-pathogen effects of tropical fungal endophytes and mycorrhizae[J]. Ecology, 2007, 88:550-558.
[5] Santoyo G, Moreno-Hagelsieb G, Orozco-Mosqueda MDC, et al. Plant growth-promoting bacterial endophytes[J]. Microbiol Res, 2016, 183:92-99.
[6] Tian Y, Amand S, Buisson D, et al. The fungal leaf endophyte Paraconiothyrium variabile specifically metabolizes the host-plant metabolome for its own benefit[J]. Phytochemistry, 2014, 108:95-101.
[7] Wu ZX, Hao ZP, Zeng Y, et al. Molecular characterization of microbial communities in the rhizosphere soils and roots of diseased and healthy Panax notoginseng[J]. Antonie van Leeuwenhoek, 2015, 108:1059-1074.
[8] Zhou LS, Tang K, Guo SX, et al. Active endophytic fungus Cladosporium sp. promoting growth and increasing salvianolic acid content of Salvia miltiorrhiza[J]. Mycosystema (菌物学报), 2018, 37:95-101.
[9] Sultan M, Schulz MH, Richard H, et al. A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome[J]. Science, 2008, 321:956-960.
[10] Wang Y, Wang HP, Chen HY, et al. Diversity and biomarker exploration of endophytic fungi communities in different varieties of Paridis Rhizoma[J]. Chin Tradit Herb Drugs (中草药), 2020, 51:4531-4537.
[11] Gweon HS, Oliver A, Taylor J, et al. PIPITS:an automated pipe-line for analyses of fungal internal transcribed spacer sequences from the illumina sequencing platform[J]. Methods Ecol Evol, 2015, 6:973-980.
[12] Yin H, Zhao Q, Sun FM, et al. Gentiopicrin-producing endophytic fungus isolated from Gentiana macrophylla[J]. Phytomedicine, 2009, 16:793-797.
[13] Mollaei S, Khanehbarndaz O, Gerami-Khashal Z, et al. Molecular identification and phytochemical screening of endophytic fungi isolated from Lithospermum officinale L. roots:a new source of shikonin[J]. Phytochemistry, 2019, 168:112116-112116.
[14] Wu LS, Dong WG, Si JP, et al. Endophytic fungi, host genotype, and their interaction influence the growth and production of key chemical components of Dendrobium catenatum[J]. Fungal Biol, 2020, 124:864-876.
[15] Wang YB, Zhang WX, Ding CJ, et al. Community structure and niche differentiation of endophytic microbiome in Populus alba×P. berolinensis under different ecological environment[J]. Sci Silv Sin (林业科学), 2020, 56:48-60.
[16] Liang XF, Tang MJ, Lv LX et al. Effects of three arbuscular mycorrhizal fungi (AMF) species on the growth, physiology, and major components of essential oil of Atractylodes lancea[J]. Chin J Ecol (生态学杂志), 2018, 37:1871-1879.
[17] Yu L, Bai JK. Advance of classification in Phoma and Phyllosticta[J]. J Jilin Agric Univ (吉林农业大学学报), 1995, 17:102-107.
[18] Chen Q. Phylogenetic and Taxonomic Studies on Didymellaceae (亚隔孢壳科的系统演化及分类学研究)[D]. Beijing:China Agricultural University, 2015.
[19] Yuan C, Guo YH, Zhang YB, et al. Secondary metabolites from Epicoccum nigrum 14 one, an endophytic fungus isolated from plant Leptogium masiaticum[J]. China J Chin Mater Med (中国中药杂志), 2019, 44:4021-4025.
相关文献:
1.陈静, 许贞, 张雪, 李妍芃, 刘春生.不同产地甘草内生真菌多样性及分离条件研究[J]. 药学学报, 2019,54(2): 373-379
2.李婷, 姜丹, 胡小松, 常晓茜, 许贞, 巩颖, 华国栋, 刘春生.基于高通量测序技术对市售柏子仁表面真菌多样性的研究[J]. 药学学报, 2019,54(11): 2100-2105