药学学报, 2021, 56(4): 1096-1099
引用本文:
方冬杰, 夏桂阳, 王玲燕, 夏欢, 孙彦斌, 林生. 醋制延胡索中一个新的原小檗碱型二聚体生物碱[J]. 药学学报, 2021, 56(4): 1096-1099.
FANG Dong-jie, XIA Gui-yang, WANG Ling-yan, XIA Huan, SUN Yan-bin, LIN Sheng. A new dimeric dehydrocorydaline alkaloid from vinegar-prepared Corydalis yanhusuo[J]. Acta Pharmaceutica Sinica, 2021, 56(4): 1096-1099.

醋制延胡索中一个新的原小檗碱型二聚体生物碱
方冬杰1, 夏桂阳2*, 王玲燕1, 夏欢2, 孙彦斌1, 林生1,2*
1. 中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050;
2. 北京中医药大学东直门医院, 中医内科学教育部和北京市重点实验室, 北京 100700
摘要:
运用大孔吸附树脂、硅胶柱色谱、反相中压柱色谱、半制备高效液相色谱等分离纯化技术,从醋制延胡索(Corydalis yanhusuo W.T.Wang)中乙酸乙酯部位分离获得1个新的通过亚甲二氧基方式连接的原小檗碱型对称二聚体生物碱,通过UV、IR、HR-ESI-MS、1D NMR和2D NMR等多种波谱分析方法确定其结构,并命名为双去氢紫堇碱A(bidehydrocorydaline A)(1)。化合物1可显著抑制脂多糖(LPS)诱导小鼠巨噬细胞RAW 264.7释放NO,IC50值为2.33 ±0.57 μmol·L-1
关键词:    延胡索      二聚体生物碱      原小檗碱      抗炎     
A new dimeric dehydrocorydaline alkaloid from vinegar-prepared Corydalis yanhusuo
FANG Dong-jie1, XIA Gui-yang2*, WANG Ling-yan1, XIA Huan2, SUN Yan-bin1, LIN Sheng1,2*
1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;
2. Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Abstract:
Bidehydrocorydaline A (1), a new dimeric alkaloid of proberberine connected by a methylenedioxy group, was isolated from the vinegar-prepared Corydalis yanhusuo by various chromatographic methods, including column chromatography over macroporous adsorption resin and silica gel, reverse-phase MPLC, and semi-preparative HPLC. Its structure was determined by spectroscopic methods, including UV, IR, HR-ESI-MS, 1D and 2D NMR. Bidehydrocorydaline A (1) showed potent inhibitory activity against LPS-induced NO production in RAW 264.7 macrophages, with an IC50 value of 2.33 ±0.57 μmol·L-1.
Key words:    Corydalis yanhusuo    dimeric alkaloid    protoberberine    anti-inflammatory   
收稿日期: 2021-01-22
DOI: 10.16438/j.0513-4870.2021-0121
基金项目: 国家自然科学基金项目(82073978,81773589,81522050);北京市自然科学基金项目(JQ18026).
通讯作者: 林生,Tel/Fax:86-10-84013404,E-mail:lsznn@126.com;夏桂阳,E-mail:nyxiaguiyang@163.com
Email: lsznn@126.com;nyxiaguiyang@163.com
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