药学学报, 2021, 56(4): 1178-1187
引用本文:
郑燕, 孙晓, 缪雨静, 江媛, 阿里穆斯, 黄林芳. 艾比湖盐碱地肉苁蓉与锁阳土壤微生物群落分析[J]. 药学学报, 2021, 56(4): 1178-1187.
ZHENG Yan, SUN Xiao, MIAO Yu-jing, JIANG Yuan, BORJIGIDAI Almaz, HUANG Lin-fang. Analysis of the soil microbial community of Cistanche deserticola and Cynomorium songaricum in the saline-alkali soil of Ebinur Lake[J]. Acta Pharmaceutica Sinica, 2021, 56(4): 1178-1187.

艾比湖盐碱地肉苁蓉与锁阳土壤微生物群落分析
郑燕1,3, 孙晓1,2, 缪雨静1,2, 江媛1,4, 阿里穆斯5, 黄林芳1,2*
1. 中国医学科学院、北京协和医学院药用植物研究所, 国家中医药管理局中药资源保护重点研究室, 北京 100193;
2. 中药资源教育部工程研究中心, 北京 100193;
3. 江西中医药大学, 江西 南昌 330000;
4. 大理大学, 云南 大理 671000;
5. 民族医药教育部重点实验室 (中央民族大学), 中央民族大学药学院, 北京 100081
摘要:
为探讨极端生境-盐碱地下两种典型寄生药用植物肉苁蓉与锁阳的土壤微生物群落特点,本文基于微生物组-生态因子策略,对新疆艾比湖的肉苁蓉与锁阳土壤进行16S扩增子测序,分析土壤微生物群落的组成,并结合核心微生物组丰度及生态气候因子进行冗余分析和相关性分析。结果表明肉苁蓉土壤微生物群落多样性显著高于锁阳,肉苁蓉与锁阳核心微生物组为海单胞菌属Marinomona、盐单胞菌科Halomonadaceae、根瘤菌目Rhizobiales、嗜盐单胞菌属Halomonas及Acidimicrobiales。可区别二者土壤微生物群落的6个特异性生物标记物为微球菌科Micrococcacea、EchinicolaGlutamicibacterGalbibacter,假交替单胞菌属PseudoalteromonasMarinobacterium_rhizophilum。冗余分析和相关性分析结果表明最干季度平均温度与最冷季度平均温度、粘土含量及土壤质地分类是影响肉苁蓉与锁阳土壤微生物群落组成的主要生态因子。本文为后期寻找肉苁蓉与锁阳的微生物分子标记,促进品质提高提供理论依据。
关键词:    肉苁蓉      锁阳      土壤微生物群落      生态因子     
Analysis of the soil microbial community of Cistanche deserticola and Cynomorium songaricum in the saline-alkali soil of Ebinur Lake
ZHENG Yan1,3, SUN Xiao1,2, MIAO Yu-jing1,2, JIANG Yuan1,4, BORJIGIDAI Almaz5, HUANG Lin-fang1,2*
1. Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China;
2. Engineering Research Center of Chinese Medicine Resource, Ministry of Education, Beijing 100193, China;
3. Jiangxi University of Traditional Chinese Medicine, Nanchang 330000, China;
4. Dali University, Dali 671000, China;
5. Key Laboratory of Ethnomedicine(Minzu University of China), Ministry of Education, School of Pharmacy, Minzu University of China, Beijing 100081, China
Abstract:
To explore the characteristics of soil microbial communities of Cistanche deserticola and Cynomorium songaricum, two typical parasitic medicinal plants that live in an extreme saline alkali environment, 16S PCR was used to sequence the soil microbial communities of C. deserticola and C. songaricum in Ebinur Lake, Xinjiang. Redundancy analysis and correlation analysis were carried out based on the abundance of core microbiome and ecoclimatic factors. The results show that the diversity of the soil microbial community of C. deserticola was significantly higher than that of C. songaricum. The core microbial groups of C. deserticola and C. songaricum were Marinomona, Halomonadaceae, Rhizobiales, Halomonas, and Acidimicrobiales. Six specific biomarkers were identified as Micrococcacea, Echinicola, Glutamicibacter, Galbibacter, Pseudoalteromonas, and Marinobacterium_rhizophilum. The results of redundancy analysis and correlation analysis show that the average temperature in the driest season and the average temperature in the coldest season, and the clay content and soil texture classification were the main ecological factors affecting the composition of these soil microbial communities. This study provides a theoretical basis for finding molecular markers of C. deserticola and C. songaricum and promoting the quality of C. deserticola and C. songaricum.
Key words:    Cistanche deserticola    Cynomorium songaricum    soil microbial community    ecological factor   
收稿日期: 2020-11-18
DOI: 10.16438/j.0513-4870.2020-1771
基金项目: 国家自然科学基金资助项目(82073960,U1812403-1);国家外专局一带一路专项(T2017052);科技部(2018FY100701).
通讯作者: 黄林芳,E-mail:lfhuang@implad.ac.cn
Email: lfhuang@implad.ac.cn
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