药学学报, 2021, 56(5): 1409-1415
引用本文:
颜海燕, 王萌, 王辉强, 张评浒, 金汉台, 李玉环. 复方鱼腥草合剂体外抗流感病毒作用研究[J]. 药学学报, 2021, 56(5): 1409-1415.
YAN Hai-yan, WANG Meng, WANG Hui-qiang, ZHANG Ping-hu, JIN Han-tai, LI Yu-huan. Effect of compound houttuynia mixture on influenza virus in vitro[J]. Acta Pharmaceutica Sinica, 2021, 56(5): 1409-1415.

复方鱼腥草合剂体外抗流感病毒作用研究
颜海燕1, 王萌1, 王辉强1, 张评浒2, 金汉台3, 李玉环1*
1. 中国医学科学院、北京协和医学院医药生物技术研究所, 中国医学科学院抗病毒药物研究重点实验室, 北京 100050;
2. 扬州大学, 江苏省人兽共患病重点实验室, 江苏 扬州 225009;
3. 浙江康恩贝中药有限公司, 浙江 丽水 323400
摘要:
复方鱼腥草合剂属于OTC甲类药物,成分为鱼腥草、黄芩、板蓝根、连翘和金银花五味药材。鱼腥草合剂作为一种中药复方制剂,药理作用广泛,具有清热解毒作用,临床用于治疗外感风热引起的咽喉疼痛、急性咽炎和扁桃腺炎。本研究对复方鱼腥草合剂体外抗流感病毒作用及初步作用机制进行了探索。采用细胞病变效应(cytopathic effects,CPE)、Western blot、实时荧光定量PCR法(quantitive reverse transcription PCR,qRT-PCR)和病毒滴度等多种方法测定了复方鱼腥草合剂的抗流感病毒作用。采用时间进程分析研究鱼腥草合剂作用于流感病毒复制周期中的阶段。结果表明,复方鱼腥草合剂体外具有广谱抗流感病毒作用,对流感病毒国际通用株、我国临床分离株耐药株、高致病性禽流感病毒H5N1和H7N9均有抑制作用,主要作用于流感病毒复制的早期阶段。
关键词:    复方鱼腥草合剂      广谱抗流感病毒      高致病禽流感      耐药株      抗流感药物     
Effect of compound houttuynia mixture on influenza virus in vitro
YAN Hai-yan1, WANG Meng1, WANG Hui-qiang1, ZHANG Ping-hu2, JIN Han-tai3, LI Yu-huan1*
1. CAMS Key Laboratory of Antiviral Drug Research, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;
2. Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China;
3. Zhejiang Conbazy Chinese Medicine Co., Ltd., Lishui 323400, China
Abstract:
Compound houttuynia mixture belongs to OTC class A medicine, which is made from Houttuynia cordata, Scutellaria baicalensis, Radix Isatidis, Forsythia, and Lonicera. As a kind of compound preparation of traditional Chinese medicine, houttuynia cordata mixture has extensive pharmacological effects, for example, clearing away heat and detoxifying, thus it is used for the sore throat, acute pharyngitis, and tonsillitis with wind-heat syndrome. In this study, the antiviral activity against influenza viruses and the primary mechanism of compound houttuynia mixture was evaluated. The antiviral effect of compound houttuynia mixture was determined by cytopathic effects (CPE), Western blot, quantitive reverse transcription PCR (qRT-PCR), and virus titer assays. The effect of houttuynia mixture on the replication cycle of influenza virus was evaluated by time-of-addition assay. In conclusion, the results showed that the compound houttuynia mixture had a broad-spectrum effect against influenza virus, including the international common influenza virus strains, the drug-resistant strains and the highly pathogenic avian influenza viruses H5N1 and H7N9. It mainly impairs the early stage of the viral replication.
Key words:    compound houttuynia mixture    a broad-spectrum antiviral effect    highly pathogenic avian influenza virus    resistant strain    anti-influenza virus agent   
收稿日期: 2020-12-08
DOI: 10.16438/j.0513-4870.2020-1881
基金项目: 国家科技重大专项“重大新药创制”资助项目(2018ZX09711003-005-004);中国医学科学院医学与健康科技创新工程(CAMS-I2M-1-010).
通讯作者: 李玉环,Tel:86-10-63010984,Fax:86-10-63017302,E-mail:yuhuanlibj@126.com
Email: yuhuanlibj@126.com
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参考文献:
[1] Le TH, Nguyen NTB. Evolutionary dynamics of highly pathogenic avian influenza A/H5N1 HA clades and vaccine implementation in Vietnam[J]. Clin Exp Vaccine Res, 2014, 3:117-127.
[2] Cox NJ, Subbarao K. Global epidemiology of influenza:past and present[J]. Annu Rev Med, 2000, 51:407-421.
[3] Doherty M, Schmidt-Ott R, Santos JI, et al. Vaccination of special populations:protecting the vulnerable[J]. Vaccine, 2016, 34:6681-6690.
[4] Pinto LH, Lamb RA. The M2 proton channels of influenza A and B viruses[J]. J Biol Chem, 2006, 281:8997-9000.
[5] Zhang Q, Zhao QJ, Xiong RS, et al. Research progress of anti-influenza virus agents[J]. Acta Pharm Sin (药学学报), 2010, 45:289-299.
[6] Xiang FF. Summary of medicinal use of Houttuynia cordata[J]. J Pract Tradit Chin Med (实用中医药杂志), 2004, 20:601.
[7] Hayashi K, Kamiya M, Hayashi T. Virucidal effects of the steam distillate from Houttuynia cordata and its components on HSV-1, influenza virus, and HIV[J]. Planta Med, 1995, 61:237-241.
[8] Liu FZ, Shi H, Shi YJ, et al. Pharmacodynamic experiment of the antivirus effect of Houttuynia Cordata Injection on influenza virus in mice[J]. Acta Pharm Sin (药学学报), 2010, 45:399-402.
[9] Zhi HJ, Zhu HY, Zhang YY, et al. In vivo effect of quantified flavonoids-enriched extract of Scutellaria baicalensis root on acute lung injury induced by influenza A virus[J]. Phytomedicine, 2019, 57:105-116.
[10] Jin J, Chen Y, Wang D, et al. The inhibitory effect of sodium baicalin on oseltamivir-resistant influenza A virus via reduction of neuraminidase activity[J]. Arch Pharm Res, 2018, 41:664-676.
[11] Chen L, Lin XJ. Clinical effect and mechanism of compound herba houttuyniae mixture for the children with macrolide-resistant myeoplasma pneumonia[J]. Int J Tradit Chin Med (国际中医中药杂志), 2018, 40:715-718.
[12] Tang ZW. Effect observation on compound Yuxingcao mixture combined with ambroxol hydrochloride oral liquid for bronchopneumonia in children[J]. J New Chin Med (新中医), 2018, 50:160-162.
[13] Qiao SP, Liu XZ, Jiang SY. Treatment of acute upper respiratory tract infection with houttuynia mixture[J]. J Southeast China National Def Med Sci (南京部队医药), 2000, 2:43-44.
[14] National Pharmacopoeia Commission.Pharmacopoeia of the People's Republic of China:Part 1(中华人民共和国药典:一部)[S]. Beijing:China Medical Science and Technology Press, 2020:1328-1329.
[15] Li L, Zhang B, Lu FG, et al. Effect of type A influenza virus on autophagy of lung macrophages and intervention of serum of Maxing Shigan decoction[J]. Chin Pharmacol Bull (中国药理学通报), 2019, 35:883-888.
[16] Yan H, Ma L, Wang H, et al. Luteolin decreases the yield of influenza A virus in vitro by interfering with the coat protein I complex expression[J]. J Nat Med, 2019, 73:487-496.
[17] Simonsen L, Clarke MJ, Schonberger LB. Pandemic versus epidemic influenza mortality:a pattern of changing age distribution[J]. J Infect Dis, 1998, 178:53-60.
[18] Hu L, Shen ZS, Wei R, et al. Effect of co-herba houttuyniae oral liquid to the stress function of radiation injured mice[J]. Pharm J China PLA (解放军药学学报), 2002, 18:44-45.
[19] Li YK, Li W, Fu CM, et al. Lonicerae japonicae flos and Lonicerae flos:a systematic review of ethnopharmacology, phytochemistry and pharmacology[J]. Phytochem Rev, 2020, 19:1-61.
[20] Zhou W, Zhang XY. Research progress of Chinese herbal medicine Radix isatidis (banlangen)[J]. Am J Chin Med, 2013, 41:743-764.
[21] Dong ZL, Lu XY, Tong XL, et al. Forsythiae Fructus:a review on its phytochemistry, quality control, pharmacology and pharmacokinetics[J]. Molecules, 2017, 22:1466-1515.
[22] Xiu SY, Zhang J, Ju H, et al. Progress on IFV drug targets and small molecule inhibitors[J]. Acta Pharm Sin (药学学报), 2020, 55:611-626.
[23] Ying K, Wang JF, Cha HJ, et al. Fingerprints analysis of compound Yuxingcao mixture by HPLC[J]. Mod Chin Med (中国现代中药), 2020. DOI:10.13313/j.issn.1673-4890.20191104003.
[24] Choi HJ, Song JH, Park KS, et al. Inhibitory effects of quercetin 3-rhamnoside on influenza A virus replication[J]. Eur J Pharm Sci, 2009, 37:329-333.
[25] Huang Y, Li J, Xu KY, et al. Research progress on anti-influenza effective components of Isatidis Radix[J]. Chin J Mod Appl Pharm (中国现代应用药学), 2019, 36:2618-2623.
[26] Zhou W. Study on the Evaluation System of Bioavailability for Chinese Medicine Orally and Its Application to Pharmaceutics Based on Flos Lonicerae-Fructus Forsythiae Herb Couples (基于"银翘"药对的中药制剂生物有效性综合评价体系的构建与应用)[D]. Nanjing:Nanjing University of Chinese Medicine, 2014.
[27] Luo X, Shen X, Hu BX. Molecular docking-based study on the anti-influenza H7N9 of Flos Lonicerae and Fructus Forsythiae[J]. Mod Tradit Chin Med (现代中医药), 2016, 36:75-78.