药学学报, 2021, 56(5): 1429-1433
引用本文:
杨丽华, 王月, 刘晓谦, 梁曜华, 李春, 王智民. 藏药甘青乌头中1个新的双二萜生物碱[J]. 药学学报, 2021, 56(5): 1429-1433.
YANG Li-hua, WANG Yue, LIU Xiao-qian, LIANG Yao-hua, LI Chun, WANG Zhi-min. A novel dimeric diterpenoid alkaloid from the Tibetan medicinal plant Aconitum tanguticum (Maxim.) Stapf[J]. Acta Pharmaceutica Sinica, 2021, 56(5): 1429-1433.

藏药甘青乌头中1个新的双二萜生物碱
杨丽华1,2, 王月1,3, 刘晓谦1, 梁曜华1, 李春1*, 王智民1*
1. 中国中医科学院中药研究所, 北京 100700;
2. 湖南科技学院, 湖南 永州 425199;
3. 广东药科大学, 广东 广州 510006
摘要:
从甘青乌头全草中分得两个双二萜生物碱,经1D,2D-NMR及HR-MS数据解析确定了它们的结构,其中1个为新化合物,命名为tanguticurine A(1),另1个为已知化合物anthoroidine B(2),二者均为首次从甘青乌头中分离得到。抗病毒实验结果显示化合物1对HCV和EV71均有较好的抑制作用,半数有效浓度(EC50)分别为15.5和9.7 μmol·L-1,并显示较低的细胞毒性。因此,化合物1是一个较好的抗病毒先导化合物,值得深入研究。
关键词:    甘青乌头      二萜生物碱      结构鉴定      抗病毒活性     
A novel dimeric diterpenoid alkaloid from the Tibetan medicinal plant Aconitum tanguticum (Maxim.) Stapf
YANG Li-hua1,2, WANG Yue1,3, LIU Xiao-qian1, LIANG Yao-hua1, LI Chun1*, WANG Zhi-min1*
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
2. Hunan University of Science and Engineering, Yongzhou 425199, China;
3. Guangdong Pharmaceutical University, Guangzhou 510006, China
Abstract:
Two dimeric diterpenoid alkaloids were isolated from the whole plant of Aconitum tanguticum (Maxim.) Stapf and their structures were elucidated by extensive analysis of 1D, 2D-NMR and HR-MS data. One is a new compound and named tanguticurine A (1), and the other is the known compound anthoroidine B (2); both were isolated from this plant for the first time. The antiviral activity of compounds 1 and 2 against HCV and EV71 were also evaluated. It was found that compound 1 had a good inhibitory effect on HCV and EV71 with EC50 values of 15.5 and 9.7 μmol·L-1, respectively, and showed low cytotoxicity. Therefore, compound 1 is a good antiviral lead compound and deserves further study.
Key words:    Aconitum tanguticum    diterpenoid alkaloid    structural elucidation    antiviral activity   
收稿日期: 2020-12-16
DOI: 10.16438/j.0513-4870.2020-1912
基金项目: 北京市自然科学基金项目(7132152);中央级公益性科研院所基本科研业务费专项资金资助项目(ZXKT17039).
通讯作者: 李春,Tel:13910436611,E-mail:cli@icmm.ac.cn;王智民,E-mail:zmwang@icmm.ac.cn
Email: cli@icmm.ac.cn;zmwang@icmm.ac.cn
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