药学学报, 2021, 56(6): 1644-1652
引用本文:
卫智权, 包传红, 陈仪新, 阎莉. 青藤碱增加配对免疫球蛋白受体B表达抑制巨噬细胞经典活化[J]. 药学学报, 2021, 56(6): 1644-1652.
WEI Zhi-quan, BAO Chuan-hong, CHEN Yi-xin, YAN Li. Sinomenine promotes paired immunoglobulin-like receptor B expression to restrain macrophage classic activation[J]. Acta Pharmaceutica Sinica, 2021, 56(6): 1644-1652.

青藤碱增加配对免疫球蛋白受体B表达抑制巨噬细胞经典活化
卫智权1#, 包传红2#, 陈仪新2, 阎莉1*
1. 广西中医药大学, 广西高校壮医方药基础与应用研究重点实验室, 广西 南宁 530001;
2. 广西中医药大学, 广西中药药效研究重点实验室, 广西 南宁 530200
摘要:
本研究以体外实验探讨青藤碱通过上调配对免疫球蛋白样受体B (paired immunoglobulin-like receptor B,PIR-B)的表达水平而抑制巨噬细胞的经典活化。以脂多糖与γ干扰素联合刺激建立巨噬细胞经典活化细胞模型。分别以实时荧光逆转录-聚合酶链反应与蛋白免疫印迹方法检测细胞PIR-B的基因与蛋白表达,以酶联免疫吸附测定法检测培养上清液肿瘤坏死因子α与白细胞介素8,流式细胞术检测M1巨噬细胞,激光扫描共聚焦显微镜观察PIR-B原位表达。结果显示,青藤碱显著增加PIR-B表达,明显减少M1巨噬细胞百分比,降低培养上清液肿瘤坏死因子α、白细胞介素8水平。以上结果提示,青藤碱可显著抑制巨噬细胞经典活化,其机制可能与其增加巨噬细胞的PIR-B表达有关,此药理作用有助于解释青藤碱治疗类风湿性关节炎的药效机制。
关键词:    青藤碱      类风湿性关节炎      配对免疫球蛋白受体B      巨噬细胞经典活化      M1巨噬细胞     
Sinomenine promotes paired immunoglobulin-like receptor B expression to restrain macrophage classic activation
WEI Zhi-quan1#, BAO Chuan-hong2#, CHEN Yi-xin2, YAN Li1*
1. Guangxi University Key Laboratory of Basic and Applied Research on Zhuang Medical Prescriptions, GuangxiTraditional Chinese Medicine University, Nanning 530001, China;
2. Guangxi Key Laboratory of Efficacy Studyon Chinese Materia Medica, Guangxi Traditional Chinese Medicine University, Nanning 530200, China
Abstract:
In this study, in vitro experiments were conducted to investigate that sinomenine inhibits the macrophage classic activation by up-regulating the expression of paired immunoglobulin-like receptor B (PIR-B). A macrophage model with classic activation was established by lipopolysaccharide and interferon-gamma co-stimulation. Real-time fluorescence reverse transcription-polymerase chain reaction was executed for evaluating the PIR-B gene expression, and Western blot for PIR-B protein expression, in macrophages, respectively. The tumor necrosis factor α and interleukin 8 in cell culture supernatant were measured by enzyme-linked immunosorbent assay. The flow cytometry was utilized to detect M1 macrophages. The PIR-B expression in situ was observed by laser scanning confocal microscope. The results showed that sinomenine significantly increased the expression of PIR-B, markedly reduced the percentage of M1 macrophages, and decreased the levels of tumor necrosis factor α and interleukin 8 in the culture supernatant. The above results indicated that sinomenine can significantly inhibit the macrophage classic activation, and its mechanism may be related to the increase of PIR-B expression in macrophages. This pharmacological effect helps explain the pharmacodynamic mechanism of sinomenine in treating rheumatoid arthritis.
Key words:    sinomenine    rheumatoid arthritis    paired immunoglobulin-like receptor B    macrophage classic activation    M1 macrophage   
收稿日期: 2020-10-19
DOI: 10.16438/j.0513-4870.2020-1623
基金项目: 国家自然科学基金资助项目(81460765);广西高校壮医方药基础与应用研究重点实验室开放课题[桂教科研(2014)6号zyfy201502].
通讯作者: 阎莉,Tel:86-771-3134025,E-mail:mimaotoo@163.com
Email: mimaotoo@163.com
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