药学学报, 2021, 56(6): 1644-1652
卫智权, 包传红, 陈仪新, 阎莉. 青藤碱增加配对免疫球蛋白受体B表达抑制巨噬细胞经典活化[J]. 药学学报, 2021, 56(6): 1644-1652.
WEI Zhi-quan, BAO Chuan-hong, CHEN Yi-xin, YAN Li. Sinomenine promotes paired immunoglobulin-like receptor B expression to restrain macrophage classic activation[J]. Acta Pharmaceutica Sinica, 2021, 56(6): 1644-1652.

卫智权1#, 包传红2#, 陈仪新2, 阎莉1*
1. 广西中医药大学, 广西高校壮医方药基础与应用研究重点实验室, 广西 南宁 530001;
2. 广西中医药大学, 广西中药药效研究重点实验室, 广西 南宁 530200
本研究以体外实验探讨青藤碱通过上调配对免疫球蛋白样受体B (paired immunoglobulin-like receptor B,PIR-B)的表达水平而抑制巨噬细胞的经典活化。以脂多糖与γ干扰素联合刺激建立巨噬细胞经典活化细胞模型。分别以实时荧光逆转录-聚合酶链反应与蛋白免疫印迹方法检测细胞PIR-B的基因与蛋白表达,以酶联免疫吸附测定法检测培养上清液肿瘤坏死因子α与白细胞介素8,流式细胞术检测M1巨噬细胞,激光扫描共聚焦显微镜观察PIR-B原位表达。结果显示,青藤碱显著增加PIR-B表达,明显减少M1巨噬细胞百分比,降低培养上清液肿瘤坏死因子α、白细胞介素8水平。以上结果提示,青藤碱可显著抑制巨噬细胞经典活化,其机制可能与其增加巨噬细胞的PIR-B表达有关,此药理作用有助于解释青藤碱治疗类风湿性关节炎的药效机制。
关键词:    青藤碱      类风湿性关节炎      配对免疫球蛋白受体B      巨噬细胞经典活化      M1巨噬细胞     
Sinomenine promotes paired immunoglobulin-like receptor B expression to restrain macrophage classic activation
WEI Zhi-quan1#, BAO Chuan-hong2#, CHEN Yi-xin2, YAN Li1*
1. Guangxi University Key Laboratory of Basic and Applied Research on Zhuang Medical Prescriptions, GuangxiTraditional Chinese Medicine University, Nanning 530001, China;
2. Guangxi Key Laboratory of Efficacy Studyon Chinese Materia Medica, Guangxi Traditional Chinese Medicine University, Nanning 530200, China
In this study, in vitro experiments were conducted to investigate that sinomenine inhibits the macrophage classic activation by up-regulating the expression of paired immunoglobulin-like receptor B (PIR-B). A macrophage model with classic activation was established by lipopolysaccharide and interferon-gamma co-stimulation. Real-time fluorescence reverse transcription-polymerase chain reaction was executed for evaluating the PIR-B gene expression, and Western blot for PIR-B protein expression, in macrophages, respectively. The tumor necrosis factor α and interleukin 8 in cell culture supernatant were measured by enzyme-linked immunosorbent assay. The flow cytometry was utilized to detect M1 macrophages. The PIR-B expression in situ was observed by laser scanning confocal microscope. The results showed that sinomenine significantly increased the expression of PIR-B, markedly reduced the percentage of M1 macrophages, and decreased the levels of tumor necrosis factor α and interleukin 8 in the culture supernatant. The above results indicated that sinomenine can significantly inhibit the macrophage classic activation, and its mechanism may be related to the increase of PIR-B expression in macrophages. This pharmacological effect helps explain the pharmacodynamic mechanism of sinomenine in treating rheumatoid arthritis.
Key words:    sinomenine    rheumatoid arthritis    paired immunoglobulin-like receptor B    macrophage classic activation    M1 macrophage   
收稿日期: 2020-10-19
DOI: 10.16438/j.0513-4870.2020-1623
基金项目: 国家自然科学基金资助项目(81460765);广西高校壮医方药基础与应用研究重点实验室开放课题[桂教科研(2014)6号zyfy201502].
通讯作者: 阎莉,Tel:86-771-3134025,E-mail:mimaotoo@163.com
Email: mimaotoo@163.com
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卫智权  在本刊中的所有文章
包传红  在本刊中的所有文章
陈仪新  在本刊中的所有文章
阎莉  在本刊中的所有文章

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