药学学报, 2021, 56(7): 1826-1831
引用本文:
孙向明, 宋辉, 赵丽珠, 胡扬, 辛科颖, 李文兰*, 丁振铎*. 菟丝子拟雌激素作用体内直接作用物质的发现[J]. 药学学报, 2021, 56(7): 1826-1831.
SUN Xiang-ming, SONG Hui, ZHAO Li-zhu, HU Yang, XIN Ke-ying, LI Wen-lan*, DING Zhen-duo*. Direct acting substances discovery of estrogen effect of Cuscuta chinensis in vivo[J]. Acta Pharmaceutica Sinica, 2021, 56(7): 1826-1831.

菟丝子拟雌激素作用体内直接作用物质的发现
孙向明, 宋辉, 赵丽珠, 胡扬, 辛科颖, 李文兰*, 丁振铎*
哈尔滨商业大学药学院, 黑龙江 哈尔滨 150076
摘要:
通过“代谢产物-效应检识”模式进行相关性分析,初步明确菟丝子在体内发挥拟雌激素作用的直接作用物质。以去势(卵巢摘除)雌性大鼠为研究对象,灌胃给予菟丝子的95%乙醇提取部位、大孔树脂40%乙醇洗脱部位、正丁醇萃取部位作为给药部位,采用UPLC/Q-TOF-MS技术建立不同给药部位、不同给药次数的血清指纹图谱。同时检测血清中雌二醇(E2)、促卵泡激素(FSH)、促黄体生成素(LH)水平,并进行双变量相关分析和灰关联度分析,筛选雌激素效应组分。结果表明,含药血清中发现9个与雌激素效应高度相关的体内直接作用物质,分别是:金丝桃苷、紫云英苷、甲基化槲皮素葡萄糖醛酸苷、槲皮素-二葡萄糖醛酸苷、槲皮素、芹菜素、异槲皮苷、山柰酚葡萄糖醛酸苷和山柰酚。基于血清谱效相关性的研究思路可初步发现菟丝子拟雌激素作用的体内直接作用物质。为菟丝子雌激素作用药效物质的揭示和质量标志物的确证提供了初步参考。本实验获得了哈尔滨商业大学伦理委员会的批准(批准号:HSDU2020-065)。
关键词:    菟丝子      拟雌激素作用      谱效相关性      超高效液相色谱-四极杆飞行时间串联质谱      体内直接作用物质     
Direct acting substances discovery of estrogen effect of Cuscuta chinensis in vivo
SUN Xiang-ming, SONG Hui, ZHAO Li-zhu, HU Yang, XIN Ke-ying, LI Wen-lan*, DING Zhen-duo*
College of Pharmacy, Harbin University of Commerce, Harbin 150076, China
Abstract:
The direct acting substances of Cuscuta chinensis in vivo were preliminarily identified through the correlation analysis of "metabolites-effect identification" model. The ovariectomized female rats were i.g administered with 95% ethanol extract part, 40% ethanol elution part and n-butanol extract part of Cuscuta chinensis. The serum fingerprints of different parts and times of administration were established by UPLC/Q-TOF-MS. At the same time, serum estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels were detected. Bivariate correlation analysis and grey correlation analysis were used to screen estrogenic components. The results showed that nine direct acting substances in vivo highly related to estrogen effect were found in the drug containing serum, which were hyperoside, astragalin, methyl quercetin glucuronide, quercetin-diglucuronide, quercetin, apigenin, isoquercitrin, kaempferol glucuronide and kaempferol. We can preliminarily screen out the direct acting substance of estrogen effect of Cuscuta chinensis in vivo based on the research idea of serum spectrum effect correlation. It provides a reliable basis for revealing the estrogeneffective substances of Cuscuta chinensis and confirming the quality markers. This experiment was approved by Harbin University of Commerce Ethics Committees (Approval No. HSDU2020-065).
Key words:    Cuscuta chinensis    estrogenic activity    spectrum effect correlation    UPLC/Q-TOF-MS    direct acting substances in vivo   
收稿日期: 2021-05-06
DOI: 10.16438/j.0513-4870.2021-0677
基金项目: 黑龙江省普通本科高等学校青年创新人才培养计划项目(UNPYSCT-2018135);哈尔滨商业大学博士科研启动基金(2019DS115).
通讯作者: 李文兰,Tel:86-451-84605022,E-mail:lwldzd@163.com;丁振铎,Tel:86-451-84866922,E-mail:dzdteacher@163.com
Email: lwldzd@163.com;dzdteacher@163.com
相关功能
PDF(400KB) Free
打印本文
0
作者相关文章
孙向明  在本刊中的所有文章
宋辉  在本刊中的所有文章
赵丽珠  在本刊中的所有文章
胡扬  在本刊中的所有文章
辛科颖  在本刊中的所有文章
李文兰*  在本刊中的所有文章
丁振铎*  在本刊中的所有文章

参考文献:
[1] Ahmad A, Tandon S, Xuan TD, et al. A review on phytoconstituents and biological activities of Cuscuta species[J]. Biomed Pharmacother, 2017, 92:772.
[2] Donnapee S, Li J, Yang X, et al. Cuscuta chinensis Lam.:a systematic review on ethnopharmacology, phytochemistry and pharmacology of an important traditional herbal medicine[J]. J Ethnopharmacol, 2014, 157:292-308.
[3] Lin MK, Lee MS, Huang HC, et al. Cuscuta chinensis and C. campestris attenuate scopolamine-induced memory deficit and oxidative damage in mice[J]. Molecules, 2018, 23:E3060.
[4] Sun SL, Guo L, Ren YC, et al. Anti-apoptosis effect of polysaccharide isolated from the seeds of Cuscuta chinensis Lam on cardiomyocytes in aging rats[J]. Mol Biol Rep, 2014, 41:6117-6124.
[5] Kang SY, Jung HW, Lee MY, et al. Effect of the semen extract of Cuscuta chinensis on inflammatory responses in LPS-stimulated BV-2 microglia[J]. Chin J Nat Med, 2014, 12:573-581.
[6] Kim HJ, Yang H, Jung DH, et al. Ameliorating effects of Cuscuta chinensis Lamak extract on hindlimb ischemia, and angiogenic-or inflammatory associated factors in ovariectomized mice[J]. Mol Med Rep, 2019, 19:3321-3329.
[7] Zhao PW, Wang DW, Niu JZ, et al. Evaluation on phytoestrogen effects of ten kinds of Chinese medicine including Flos Carthami[J]. Chin J Chin Mater Med (中国中药杂志), 2007, 32:436-439.
[8] Bittner V. Menopause, age and cardiovascular risk:a complex relationship[J]. J Am Coll Cardiol, 2009, 54:2374.
[9] Liu W, Tao T, Qi YC. Advantages and disadvantages of female hormone replacement therapy[J]. Chin J Endocrinol Metab (中华内分泌代谢杂志), 2020, 36:277-282.
[10] Decensi A, Bonanni B, Maisonneuve P, et al. A phase-Ⅲ prevention trial of low-dose tamoxifen in postmenopausal hormone replacement therapy users:the HOT study[J]. Ann Oncol, 2013, 24:2753.
[11] Sun XM, Song H, Yan XJ, et al. Screening and determination of estrogen-like quality markers of Cuscuta chinensis[J]. Chin Tradit Herb Drugs (中草药), 2020, 51:2671-2679.
[12] Zhang Y, Hua F, Ding K, et al. Angiogenesis changes inovariectomized rats with osteoporosis treated with estrogen replacement therapy[J]. Bio Res Int, 2019, 2019:1-9.
[13] Wang YD, Zhang ZP, Liu SB. Detection and clinical significance of serum FSH, LH and E2 in perimenopausal women[J]. J Radioimmunol, 2009, 22:123-124.
[14] Morand C, Creapy V, Manach C, et al. Plasma metabolites of quercetin and their antioxidant properties[J]. Am J Physiol, 1998, 275:212-219.
[15] Wang ZX. Determination of quercetin and its main metabolites in plasma by HPLC-MS[J]. Chin Tradit Pat Med (中成药), 2012, 34:750-753.
[16] Wei Q, Liu KM. Research progress on spectrum-effect relationship of traditional Chinese medicine[J]. Tradit Chin Drug Res Pharmacol (中药新药与临床药理), 2019, 30:634-638.
[17] Qu Y, Wang L, Guo W. Screening and identification of antipyretic components in the postfrost leaves of Morus alba based on multivariable and continuous-index spectrum-effect correlation[J]. J Anal Methods Chem, 2019:8796276. DOI:10.1155/2019/8796276.
[18] Hong Y, Liu XH, Chen YL, et al. Multi-index integrated evaluation of spectrum-effect relationship on immunocompetence of Radix Astragali[J]. Acta Pharm Sin (药学学报), 2017, 52:785-789.
[19] Wang XL, Liu X, Han YQ, et al. Overview on main research method of effective material basis of Chinese materia medica[J]. Chin Tradit Herb Drugs (中草药), 2018, 49:941-947.
[20] Zhu SC, Jiang ZX, Li JJ, et al. Overview research status in serum spectrum-effect of Chinese materia medica[J]. Chin Tradit Herb Drugs (中草药), 2020, 51:3569-3574.
[21] Cai YY, Huang XH. Effect of hormone replacement therapy on perimenopausal syndrome and its influence on serum estradiol, follicle stimulating hormone and luteinizing hormone[J]. Matern Child Health Care China (中国妇幼保健), 2015, 30:5396-5398.