药学学报, 2021, 56(8): 2039-2047
赵梦, 李思敏, 张蕾, 丛明慧, 胡立宏, 乔宏志. 植物来源囊泡及其生物医学应用研究进展[J]. 药学学报, 2021, 56(8): 2039-2047.
ZHAO Meng, LI Si-min, ZHANG Lei, CONG Ming-hui, HU Li-hong, QIAO Hong-zhi. Research progress of plant-derived vesicles and their biomedical applications[J]. Acta Pharmaceutica Sinica, 2021, 56(8): 2039-2047.

赵梦1, 李思敏1, 张蕾1, 丛明慧1, 胡立宏1, 乔宏志1,2*
1. 南京中医药大学药学院, 江苏省中药功效物质重点实验室, 江苏 南京 210023;
2. 江苏省中药高效给药系统工程技术研究中心, 江苏 南京 210023
植物细胞外囊泡(extracellular vesicles,EVs)是一种由植物细胞分泌的,以脂质双层为基本骨架、包裹各种蛋白和核酸等活性物质的膜性小泡,对植物的生长发育、组织修复和自体防御等过程起重要作用。近年来,参照EVs的分离方法,从植物样本中制备得到细胞外囊泡样纳米粒(extracellular vesicle-like nanoparticles,EVNs)具有与EVs相似的结构组成,也显示出独特的活性功能。本文将上述结构统称为植物来源囊泡(plant-derived vesicles,PDVs),系统介绍了PDVs生源途径、分离表征方法和体内外性质,讨论其作为天然治疗剂和药物功能载体等方面的生物医学应用,最后对该领域存在的问题及未来发展方向提出观点和建议。
关键词:    细胞外囊泡      外泌体      纳米粒      植物      中药      药理作用      药物递送      结构中药学      物质基础     
Research progress of plant-derived vesicles and their biomedical applications
ZHAO Meng1, LI Si-min1, ZHANG Lei1, CONG Ming-hui1, HU Li-hong1, QIAO Hong-zhi1,2*
1. Jiangsu Key Laboratory for Functional Substance of Chinese Medicine, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China;
2. Jiangsu Engineering Research Center for Efficient Delivery System of TCM, Nanjing 210023, China
Plant-derived extracellular vesicles (EVs) are membranous vesicles secreted by plants, which include lipid bilayer as the basic framework and encapsulate various proteins, nucleic acid and other active substances. They play an important role in plant growth and development, tissue repair and self-defense. In recent years, extracellular vesicle-like nanoparticles (EVNs) are prepared from plant samples referring to the separation method of EVs and show unique functions. In this review, the above structures are collectively called plant-derived vesicles (PDVs). The biogenesis, separation and characterization methods, in vivo and in vitro properties of PDVs have been reviewed. The biomedical applications of PDVs as natural therapeutic agents and functional drug carriers are described, and finally some opinions on the existing problems and future prospect in this field are put forward.
Key words:    extracellular vesicle    exosome    nanoparticle    plant    Chinese medicine    pharmacological action    drug delivery    structural Chinese medicine    pharmacodynamic substance basis   
收稿日期: 2020-09-18
DOI: 10.16438/j.0513-4870.2020-1514
基金项目: 中国科协青年人才托举工程项目(2017QNRC001);中央本级重大增减支项目(2060302-1907-04);江苏省高等学校自然科学研究重大项目(20KJA360004);江苏省六大人才高峰项目(SWYY-057);江苏省青蓝工程项目;江苏省研究生创新工程项目(SJCX20_0547,SJCX20_0546,KYCX20_1497).
通讯作者: 乔宏志,Tel:86-25-85811050,E-mail:qiaohz@njucm.edu.cn
Email: qiaohz@njucm.edu.cn
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李思敏  在本刊中的所有文章
张蕾  在本刊中的所有文章
丛明慧  在本刊中的所有文章
胡立宏  在本刊中的所有文章
乔宏志  在本刊中的所有文章

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