药学学报, 2021, 56(8): 2204-2216
引用本文:
刘龙婵, 李依璠, 张方丽, 李林楠, 王峥涛, 杨莉. 解吸电喷雾电离质谱技术在天然药物分析中的应用进展[J]. 药学学报, 2021, 56(8): 2204-2216.
LIU Long-chan, LI Yi-fan, ZHANG Fang-li, LI Lin-nan, WANG Zheng-tao, YANG Li. Desorption electrospray ionization-mass spectrometry in the analysis of natural medicines[J]. Acta Pharmaceutica Sinica, 2021, 56(8): 2204-2216.

解吸电喷雾电离质谱技术在天然药物分析中的应用进展
刘龙婵1, 李依璠1, 张方丽1, 李林楠1*, 王峥涛1,2, 杨莉1,2*
1. 上海中医药大学中药研究所, 中药标准化教育部重点实验室, 国家中医药管理局中药新资源与质量评价重点实验室, 上海 201203;
2. 上海中药标准化研究中心, 上海 201203
摘要:
解吸电喷雾电离质谱(desorption electrospray ionization-mass spectrometry,DESI-MS)是一种近年来新兴的原位电离质谱分析技术,其离子化过程发生于常压敞开式大气环境中,具有样品前处理简单、分析快速、检测灵敏等特点,在生物医学、药物分析、食品安全、环境监测以及材料表征等方面应用广泛。天然药物如中草药中含有种类繁复的化学组成,对其开展提取、分离鉴定和体内外药效评价,特别是针对药效显著活性成分的研究,长期受到人们的持续关注。近年来,随着DESI-MS技术的不断发展,为直接和快速分析天然药物中活性成分提供了许多新的机会。本文概述了DESI-MS技术的原理、特点、影响因素及技术进展,并系统总结了该技术在中草药及其他具有药理活性的植物样品等天然药物中的研究应用进展情况,进一步对该领域的应用前景做了展望。
关键词:    解吸电喷雾电离质谱      中草药      天然药物      质谱成像      质量控制     
Desorption electrospray ionization-mass spectrometry in the analysis of natural medicines
LIU Long-chan1, LI Yi-fan1, ZHANG Fang-li1, LI Lin-nan1*, WANG Zheng-tao1,2, YANG Li1,2*
1. The MOE Key Laboratory of Standardization of Chinese Medicines and the SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;
2. Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai 201203, China
Abstract:
Desorption electrospray ionization mass spectrometry (DESI-MS) is a newly emerging in-situ ionization mass spectrometry analysis technology. The ionization process occurs in an open ambient environment at atmospheric pressure, and has the characteristics of simple sample pretreatment, quick and sensitive analysis, and is widely used in biomedicine, pharmaceutical analysis, food safety, environmental monitoring, and material characterization. Natural medicines, such as Chinese herbal medicines, contain a variety of chemical components. Extraction, separation, identification, and in vitro and in vivo efficacy evaluation of natural medicines, especially research on active ingredients with significant efficacy, have received long-term attention. The development of DESI-MS technology provides many new opportunities for direct and rapid analysis of active ingredients in natural medicines. This article briefly introduces the principles, characteristics, influencing factors, and technical progress of DESI-MS technology, and systematically summarizes progress in the research and application of this technology to natural medicines such as Chinese herbal medicines and other plant samples with pharmacological activity. The future application prospects in this field are further presented.
Key words:    desorption electrospray ionization-mass spectrometry    Chinese herbal medicine    natural medicine    imaging mass spectrometry    quality control   
收稿日期: 2021-02-02
DOI: 10.16438/j.0513-4870.2021-0204
基金项目: 国家自然科学基金资助项目(21904089,82074011);上海市科委科研计划项目(19401900200,19DZ2201500);扬帆计划(19YF1448800);上海市进一步加快中医药事业发展三年行动计划项目[ZY(2018-2020)-CCCX-5002].
通讯作者: 李林楠,E-mail:linnanli@shutcm.edu.cn;杨莉,E-mail:yl7@shutcm.edu.cn
Email: linnanli@shutcm.edu.cn;yl7@shutcm.edu.cn
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参考文献:
[1] Wang Y. Research and analysis on chemical constituents of natural medicines[J]. Mod Salt Chem Ind (现代盐化工), 2020, 47:34-35.
[2] Huang X, Liu WL, Zhang Y, et al. Application of ambient ionization mass spectrometry in Chinese herbal medicine research[J]. J Chin Mass Spectrom Soc (质谱学报), 2017, 38:1-10.
[3] Liu YF, Liu YM, Dong J, et al. The study of high-performance liquid chromatography methods for the separation and analysis of traditional Chinese medicines[J]. Sci China (Series B) (中国科学B辑), 2009, 39:678-686.
[4] Zhu MZ, Chen GL, Wu JL, et al. Recent development in mass spectrometry and its hyphenated techniques for the analysis of medicinal plants[J]. Phytochem Anal, 2018, 29:365-374.
[5] Wong M YM, So PK, Yao ZP. Direct analysis of traditional Chinese medicines by mass spectrometry[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2016, 1026:2-14.
[6] Ge JY, Hu DJ, Zhang Y, et al. Analytical capabilities of MALDIMSI and its potential applications in medicinal plants[J]. Acta Pharm Sin (药学学报), 2019, 54:1179-1189.
[7] Takats Z, Wiseman JM, Gologan B, et al. Mass spectrometry sampling under ambient conditions with desorption electrospray ionization[J]. Science, 2004, 306:471-473.
[8] Tian M, Li LN, Yang YG, et al. Rapid characterization of saponins in fresh and steamed notoginseng root slices by liquid extraction, surface analysis-mass spectrometry[J]. Acta Pharm Sin (药学学报), 2020, 55:123-130.
[9] Yoshimura Y, Zaima N. Application of mass spectrometry imaging for visualizing food components[J]. Foods, 2020, 9:575-594.
[10] Li N, Nie H, Jiang L, et al. Recent advances of ambient ionization mass spectrometry imaging in clinical research[J]. J Sep Sci, 2020, 43:3146-3163.
[11] Kuo TH, Dutkiewicz EP, Pei J, et al. Ambient ionization mass spectrometry today and tomorrow:embracing challenges and opportunities[J]. Anal Chem, 2020, 92:2353-2363.
[12] Gentili A, Fanali S, Rocca LM. Desorption electrospray ionization mass spectrometry for food analysis[J]. Trac-Trend Anal Chem, 2019, 115:162-173.
[13] Tose LV, Murgu M, Vaz BG, et al. Application of atmospheric solids analysis probe mass spectrometry (ASAP-MS) in petroleomics:analysis of condensed aromatics standards, crude oil, and paraffinic fraction[J]. J Am Soc Mass Spectr, 2017, 28:2401-2407.
[14] Bennett RV, Fernandez FM. Desorption electrospray ionization imaging of small organics on mineral surfaces[J]. Methods Mol Biol, 2015, 1203:79-89.
[15] Da Costa C, Reynolds JC, Whitmarsh S, et al. The quantitative surface analysis of an antioxidant additive in a lubricant oil matrix by desorption electrospray ionization mass spectrometry[J]. Rapid Commun Mass Spectrom, 2013, 27:2420-2424.
[16] Parrot D, Papazian S, Foil D, et al. Imaging the unimaginable:desorption electrospray ionization-imaging mass spectrometry (DESI-IMS) in natural product research[J]. Planta Med, 2018, 84:9-10.
[17] Joyce NI, Eady CC, Silcock P, et al. Fast phenotyping of LFSsilenced (tearless) onions by desorption electrospray ionization mass spectrometry (DESI-MS) [J]. J Agric Food Chem, 2013, 61:1449-1456.
[18] Zhou YF. Development and Applications of Several Ambient Mass Spectrometry Technology (几种常压快速质谱技术及其研究应用) [D]. Nanchang:East China University of Technology, 2014.
[19] Honarvar E, Venter AR. Comparing the effects of additives on protein analysis between desorption electrospray (DESI) and electrospray ionization (ESI) [J]. J Am Soc Mass Spectr, 2018, 29:2443-2455.
[20] Costa AB, Cooks RG. Simulated splashes:elucidating the mechanism of desorption electrospray ionization mass spectrometry[J]. Chem Phys Lett, 2008, 464:1-8.
[21] Lanekoff I, Thomas M, Carson JP, et al. Imaging nicotine in rat brain tissue by use of nanospray desorption electrospray ionization mass spectrometry[J]. Anal Chem, 2013, 85:882-889.
[22] Dill AL, Eberlin LS, Costa AB, et al. Data quality in tissue analysis using desorption electrospray ionization[J]. Anal Bioanal Chem, 2011, 401:1949-1961.
[23] Xue J, Bai Y, Liu H. Recent advances in ambient mass spectrometry imaging[J]. Trac-Trend Anal Chem, 2019, 120:115659.
[24] Schwab NV, Ore MO, Eberlin MN, et al. Functionalized porous silicon surfaces as DESI-MS substrates for small molecules analysis[J]. Anal Chem, 2014, 86:11722-11726.
[25] Penna A, Careri M, Spencer ND, et al. Effects of tailored surface chemistry on desorption electrospray ionization mass spectrometry:a surface-analytical study by XPS and AFM[J]. J Am Soc Mass Spectr, 2015, 26:1311-1319.
[26] Penna A, Elviri L, Careri M, et al. Investigation of novel sol-gel hydrophobic surfaces for desorption electrospray ionizationmass spectrometry analysis[J]. Anal Bioanal Chem, 2011, 400:1515-1523.
[27] Ho YN, Shu LJ, Yang YL. Imaging mass spectrometry for metabolites:technical progress, multimodal imaging, and biological interactions[J]. Wiley Interdiscip Rev Syst Biol Med, 2017, 9:e1387.
[28] Kooijman PC, Nagornov KO, Kozhinov AN, et al. Increased throughput and ultra-high mass resolution in DESI FT-ICR MS imaging through new-generation external data acquisition system and advanced data processing approaches[J]. Sci Rep, 2019, 9:8-19.
[29] Takats Z, Wiseman JM, Cooks RG. Ambient mass spectrometry using desorption electrospray ionization (DESI):instrumentation, mechanisms and applications in forensics, chemistry, and biology[J]. J Mass Spectrom, 2005, 40:1261-1275.
[30] Wiseman JM, Ifa DR, Venter A, et al. Ambient molecular imaging by desorption electrospray ionization mass spectrometry[J]. Nat Protoc, 2008, 3:517-524.
[31] Bodzon-Kulakowska A, Drabik A, Ner J, et al. Desorption electrospray ionisation (DESI) for beginners how to adjust settings for tissue imaging[J]. Rapid Commun Mass Spectrom, 2014, 28:1-9.
[32] Feider CL, Dehoog RJ, Sans M, et al. DESI spray stability in the negative ion mode is dependent on relative humidity[J]. J Am Soc Mass Spectr, 2019, 30:376-380.
[33] Newsome GA, Ackerman LK, Johnson KJ. Humidity effects on fragmentation in plasma-based ambient ionization sources[J]. J Am Soc Mass Spectr, 2016, 27:135-143.
[34] Kompauer M, Heiles S, Spengler B. Atmospheric pressure MALDI mass spectrometry imaging of tissues and cells at 1.4-μm lateral resolution[J]. Nat Methods, 2017, 14:90-96.
[35] Yang PY, Liu YC, Zhao HY, et al. Recent progress in mass spectrometry based molecular imaging[J]. Sci Sin Vit (中国科学:生命科学), 2020, 50:1237-1255.
[36] Comi TJ, Ryu SW, Perry RH. Synchronized desorption electrospray ionization mass spectrometry imaging[J]. Anal Chem, 2016, 88:1169-1175.
[37] Lanekoff I, Heath BS, Liyu A, et al. Automated platform for high-resolution tissue imaging using nanospray desorption electrospray ionization mass spectrometry[J]. Anal Chem, 2012, 84:8351-8356.
[38] Nazari M, Muddiman DC. Cellular-level mass spectrometry imaging using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) by oversampling[J]. Anal Bioanal Chem, 2015, 407:2265-2271.
[39] Wu C, Ifa DR, Manicke NE, et al. Rapid, direct analysis of cholesterol by charge labeling in reactive desorption electrospray ionization[J]. Anal Chem, 2009, 81:7618-7624.
[40] Zhou J, Yao S, Qian R, et al. Observation of allicin-cysteine complex by reactive desorption electrospray ionization mass spectrometry for garlic[J]. Rapid Commun Mass Spectrom, 2008, 22:3334-3337.
[41] Ifa DR, Wu CP, Ouyang Z, et al. Desorption electrospray ionization and other ambient ionization methods:current progress and preview[J]. Analyst, 2010, 135:669-681.
[42] Myung S, Wiseman JM, Valentine SJ, et al. Coupling desorption electrospray ionization with ion mobility/mass spectrometry for analysis of protein structure:evidence for desorption of folded and denatured states[J]. J Phys Chem B, 2006, 110:5045-5051.
[43] Dixon RB, Bereman MS, Muddiman DC, et al. Remote mass spectrometric sampling of electrosprayand desorption electrospragenerated ions using an air ejector[J]. J Am Soc Mass Spectr, 2007, 18:1844-1847.
[44] Zhang S, Shin YS, Mayer R, et al. On-probe pyrolysis desorption electrospray ionization (DESI) mass spectrometry for the analysis of non-volatile pyrolysis products[J]. J Anal Appl Pyrolysis, 2007, 80:353-359.
[45] Denes J, Katona M, Hosszu A, et al. Analysis of biological fluids by direct combination of solid phase extraction and desorption electrospray ionization mass spectrometry[J]. Anal Chem, 2009, 81:1669-1675.
[46] Lloyd JA, Harron AF, Mcewen CN. Combination atmospheric pressure solids analysis probe and desorption electrospray ionization mass spectrometry ion source[J]. Anal Chem, 2009, 81:9158-9162.
[47] Nyadong L, Galhena AS, Fernandez FM. Desorption electrospray/metastable-induced ionization:a flexible multimode ambient ion generation technique[J]. Anal Chem, 2009, 81:7788-7894.
[48] Cotte-Rodriguez I, Mulligan CC, Cooks G. Non-proximate detection of small and large molecules by desorption electrospray ionization and desorption atmospheric pressure chemical ionization mass spectrometry:instrumentation and applications in forensics, chemistry, and biology[J]. Anal Chem, 2007, 79:7069-7077.
[49] Ma X, Zhao M, Lin Z, et al. Versatile platform employing desorption electrospray ionization mass spectrometry for highthroughput analysis[J]. Anal Chem, 2008, 80:6131-6136.
[50] Haddad R, Sparrapan R, Eberlin MN. Desorption sonic spray ionization for (high) voltage-free ambient mass spectrometry[J]. Rapid Commun Mass Spectrom, 2006, 20:2901-2915.
[51] Huang JP, Zhang J, Liu D, et al. A positive/negative ion modeswitched mass spectrometry imaging method and its application in metabolomics analysis of whole-body animals[J]. J Instrum Anal (分析测试学报), 2021, 40:256-262.
[52] Lendor S, Gomez-Rios GA, Boyaci E, et al. Space-resolved tissue analysis by solid-phase microextraction coupled to highresolution mass spectrometry via desorption electrospray ionization[J]. Anal Chem, 2019, 91:10141-10148.
[53] Bodzon-Kulakowska A, Cichon T, Golec A, et al. DESI-MS as a tool for direct lipid analysis in cultured cells[J]. Cytotechnology, 2015, 67:1085-1091.
[54] Eberlin LS. DESI-MS imaging of lipids and metabolites from biological samples[J]. Methods Mol Biol, 2014, 1198:299-311.
[55] Eberlin LS, Ferreira CR, Dill AL, et al. Desorption electrospray ionization mass spectrometry for lipid characterization and biological tissue imaging[J]. Biochim Biophys Acta Mol Cell Biol Lipids, 2011, 1811:946-960.
[56] Lostun D, Perez CJ, Licence P, et al. Reactive DESI-MS Imaging of biological tissues with dicationic ion-pairing compounds[J]. Anal Chem, 2015, 87:3286-3293.
[57] Suni NM, Aalto H, Kauppila TJ, et al. Analysis of lipids with desorption atmospheric pressure photoionization-mass spectrometry (DAPPI-MS) and desorption electrospray ionizationmass spectrometry (DESI-MS) [J]. J Mass Spectrom, 2012, 47:611-619.
[58] Weston DJ. Ambient ionization mass spectrometry:current understanding of mechanistic theory; analytical performance and application areas[J]. Analyst, 2010, 135:661-668.
[59] Zhang JI, Talaty N, Costa AB, et al. Rapid direct lipid profiling of bacteria using desorption electrospray ionization mass spectrometry[J]. Int J Mass Spectrom, 2011, 301:37-44.
[60] Talaty N, Takats Z, Cooks RG. Rapid in situ detection of alkaloids in plant tissue under ambient conditions using desorption electrospray ionization[J]. Analyst, 2005, 130:1624-1633.
[61] Chen HW, Zheng J, Wang WP, et al. Desorption electrospray ionization mass spectrometry for fast detection of alkaloids in fructus evodiae[J]. Chin J Anal Chem (分析化学), 2009, 37:237-241.
[62] Srimany A, Ifa DR, Naik HR, et al. Direct analysis of camptothecin from Nothapodytes nimmoniana by desorption electrospray ionization mass spectrometry (DESI-MS) [J]. Analyst, 2011, 136:3066-3068.
[63] Conceicao RS, Reis IMA, Cerqueira APM, et al. Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC-DESI-MSn[J]. Phytochem Anal, 2020, 31:711-721.
[64] Klejdus B, Kovacik J. Quantification of phenols in cinnamon:a special focus on "total phenols" and phenolic acids including DESI-Orbitrap MS detection[J]. Ind Crops Prod, 2016, 83:774-780.
[65] Jackson AU, Tata A, Wu CP, et al. Direct analysis of Stevia leaves for diterpene glycosides by desorption electrospray ionization mass spectrometry[J]. Analyst, 2009, 134:867-874.
[66] Müller T, Oradu S, Ifa DR, et al. Direct plant tissue analysis and imprint imaging by desorption electrospray ionization mass spectrometry[J]. Anal Chem, 2011, 83:5754-5761.
[67] Van Berkel GJ, Tomkins BA, Kertesz V. Thin-layer chromatography/desorption electrospray ionization mass spectrometry:investigation of goldenseal alkaloids[J]. Anal Chem, 2007, 79:2778-2789.
[68] Laub A, Sendatzki AK, Palfner G, et al. HPTLC-DESI-HRMSbased profiling of anthraquinones in complex mixtures-a proofof-concept study using crude extracts of Chilean mushrooms[J]. Foods, 2020, 9:156-165.
[69] Kennedy JH, Wiseman JM. Direct analysis of Salvia divinorum leaves for salvinorin A by thin layer chromatography and desorption electrospray ionization multi-stage tandem mass spectrometry[J]. Rapid Commun Mass Spectrom, 2010, 24:1305-1311.
[70] Bagatela BS, Lopes AP, Cabral EC, et al. High-performance thin-layer chromatography/desorption electrospray ionization mass spectrometry imaging of the crude extract from the peels of Citrus aurantium L. (Rutaceae) [J]. Rapid Commun Mass Spectrom, 2015, 29:1530-1534.
[71] Sun B, Zhao YF, Zhu GW, et al. Application of DESI-MSI in quality control of Banxia Xiexin tang[J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志), 2020, 26:117-128.
[72] Qu YZ, Sun B, Zhu GW, et al. Study on application of DESIMSI in quality control of classical famous prescription Shaoyao Gancao decoction[J]. Chin Tradit Herb Drugs (中草药), 2020, 51:3433-3443.
[73] Mamun MA, Gonzalez TV, Islam A, et al. Analysis of potential anti-aging beverage Pru, a traditional Cuban refreshment, by desorption electrospray ionization-mass spectrometry and FTICR tandem mass spectrometry[J]. J Food Drug Anal, 2019, 27:833-840.
[74] Tata A, Perez CJ, Hamid TS, et al. Analysis of metabolic changes in plant pathosystems by imprint imaging DESI-MS[J]. J Am Soc Mass Spectrom, 2015, 26:641-648.
[75] de Abreu LB, Augusti R, Schmidt L, et al. Desorption electrospray ionization mass spectrometry (DESI-MS) applied to the speciation of arsenic compounds from fern leaves[J]. Anal Bioanal Chem, 2013, 405:7643-7651.
[76] Hemalatha RG, Naik HR, Mariappa V, et al. Rapid detection of Fusarium wilt in basil (Ocimum sp.) leaves by desorption electrospray ionization mass spectrometry (DESI MS) imaging[J]. Rsc Adv, 2015, 5:50512-50522.
[77] Gerbig S, Brunn HE, Spengler B, et al. Spatially resolved investigation of systemic and contact pesticides in plant material by desorption electrospray ionization mass spectrometry imaging (DESI-MSI) [J]. Anal Bioanal Chem, 2015, 407:7379-7389.
[78] Yang YG, Yang YB, Qiu H, et al. Localization of constituents for determining the age and parts of ginseng through ultraperfomance liquid chromatography quadrupole/time of flight-mass spectrometry combined with desorption electrospray ionization mass spectrometry imaging[J]. J Pharm Biomed Anal, 2020, 193:113722.
[79] Nyadong L, Hohenstein EG, Galhena A, et al. Reactive desorption electrospray ionization mass spectrometry (DESI-MS) of natural products of a marine alga[J]. Anal Bioanal Chem, 2009, 394:245-254.
[80] Parrot D, Blumel M, Utermann C, et al. Mapping the surface microbiome and metabolome of brown seaweed fucus vesiculosus by amplicon sequencing, integrated metabolomics and imaging techniques[J]. Sci Rep, 2019, 9:17.
[81] Li B, Hansen SH, Janfelt C. Direct imaging of plant metabolites in leaves and petals by desorption electrospray ionization mass spectrometry[J]. Int J Mass Spectrom, 2013, 348:15-22.
[82] Kumara PM, Shaanker RU, Pradeep T. UPLC and ESI-MS analysis of metabolites of Rauvolfia tetraphylla L. and their spatial localization using desorption electrospray ionization (DESI) mass spectrometric imaging[J]. Phytochemistry, 2019, 159:20-29.
[83] Freitas JRLE, Vendramini PH, Melo JOE, et al. Assessing the spatial distribution of key flavonoids in Mentha×piperita leaves:an application of desorption electrospray ionization mass spectrometry imaging (DESI-MSI) [J]. J Braz Chem Soc, 2019, 30:1437-1446.
[84] Cabral EC, Mirabelli MF, Perez CJ, et al. Blotting assisted by heating and solvent extraction for desi-ms imaging[J]. J Am Soc Mass Spectr, 2013, 24:956-965.
[85] Li B, Bjarnholt N, Hansen SH, et al. Characterization of barley leaf tissue using direct and indirect desorption electrospray ionization imaging mass spectrometry[J]. J Mass Spectrom, 2011, 46:1241-1246.
[86] Thunig J, Hansen SH, Janfelt C. Analysis of secondary plant metabolites by indirect desorption electrospray ionization imaging mass spectrometry[J]. Anal Chem, 2011, 83:3256-3259.
[87] Suma HK, Kumar V, Kumara PM, et al. Spatial and temporal distribution pattern of camptothecin in seeds and fruits of Pyrenacantha volubilis Hook. (Icacinaceae) during different fruit developmental stages[J]. Curr Sci, 2017, 112:1034-1038.
[88] Li B, Knudsen C, Hansen NK, et al. Visualizing metabolite distribution and enzymatic conversion in plant tissues by desorption electrospray ionization mass spectrometry imaging[J]. Plant J, 2013, 74:1059-1071.
[89] Freitas JRL, Vendramini PH, Melo JOF, et al. An appraisal on the source-to-sink relationship in plants:an application of desorption electrospray ionization mass spectrometry imaging[J]. J Braz Chem Soc, 2018, 29:17-23.
[90] Kumara PM, Srimany A, Ravikanth G, et al. Ambient ionization mass spectrometry imaging of rohitukine, a chromone anti-cancer alkaloid, during seed development in Dysoxylum binectariferum Hook.f (Meliaceae) [J]. Phytochemistry, 2015, 116:104-110.
[91] Kumara PM, Srimany A, Arunan S, et al. Desorption electrospray ionization (DESI) mass spectrometric imaging of the distribution of rohitukine in the seedling of Dysoxylum binectariferum Hook. F[J]. PLoS One, 2016, 11:0158099.
[92] Kato L, Moraes AP, Alves de Oliveira CM, et al. The spatial distribution of alkaloids in Psychotria prunifolia (Kunth) Steyerm and Palicourea coriacea (Cham.) K. Schum leaves analysed by desorption electrospray ionisation mass spectrometry imaging[J]. Phytochem Anal, 2018, 29:69-76.
[93] Hemalatha RG, Pradeep T. Understanding the molecular signatures in leaves and flowers by desorption electrospray ionization mass spectrometry (DESI MS) imaging[J]. J Agric Food Chem, 2013, 61:7477-7487.
[94] Kucharikova A, Kimakova K, Janfelt C, et al. Interspecific variation in localization of hypericins and phloroglucinols in the genus Hypericum as revealed by desorption electrospray ionization mass spectrometry imaging[J]. Physiol Plant, 2016, 157:2-12.
[95] Chen J, Zhang F, Zhang R, et al. Determination of hyoscyamine, scopolamine and anisodamine in Datura innoxia by LC-MS/MS[J]. J Jinggangshan Univ Nat Sci (井冈山大学学报·自然科学版), 2019, 40:92-95.
[96] Zhang JJ. Ginseng Genome Research and Multi-omics Data Elaboration on the Ginsenosides Biosynthesis (基于人参全基因组的多组学数据解析人参皂苷生物合成) [D]. Wuhan:Hubei University of Chinese Medicine, 2018.
[97] Claude E, Tower M, Lafont R, et al. High performance thin-layer chromatography of plant ecdysteroids coupled with desorption electrospray ionisation-ion mobility-time of flight high resolution mass spectrometry (HPTLC/DESI/IM/ToFMS) [J]. Chromatographia, 2020, 83:1029-1035.
[98] Janfelt C. Imaging of plant materials using indirect desorption electrospray ionization mass spectrometry[J]. Methods Mol Biol, 2015, 1203:91-97.
[99] Lane AL, Nyadong L, Galhena AS, et al. Desorption electro-spray ionization mass spectrometry reveals surface-mediated antifungal chemical defense of a tropical seaweed[J]. Proc Natl Acad Sci U S A, 2009, 106:7314-7319.
[100] Esquenazi E, Dorrestein PC, Gerwick WH. Probing marine natural product defenses with DESI-imaging mass spectrometry[J]. Proc Natl Acad Sci U S A, 2009, 106:7269-7270.
[101] Andras TD, Alexander TS, Gahlena A, et al. Seaweed allelopathy against coral:surface distribution of a seaweed secondary metabolite by imaging mass spectrometry[J]. J Chem Ecol, 2012, 38:1203-1214.
[102] Tata A, Perez CJ, Ore MO, et al. Evaluation of imprint DESI-MS substrates for the analysis of fungal metabolites[J]. Rsc Adv, 2015, 5:75458-75464.
[103] Ifa DR, Srimany A, Eberlin LS, et al. Tissue imprint imaging by desorption electrospray ionization mass spectrometry[J]. Anal Methods, 2011, 3:1910-1912.