药学学报, 2021, 56(8): 2248-2251
引用本文:
王莎莎, 陈曦, 王凤舞, 徐亚平, 叶桐, 申丽. 海带内生菌Alternaria sp.W-1次生代谢产物研究[J]. 药学学报, 2021, 56(8): 2248-2251.
WANG Sha-sha, CHEN Xi, WANG Feng-wu, XU Ya-ping, YE Tong, SHEN Li. Secondary metabolites of endophyte Alternaria sp. W-1 from Laminaria japonica[J]. Acta Pharmaceutica Sinica, 2021, 56(8): 2248-2251.

海带内生菌Alternaria sp.W-1次生代谢产物研究
王莎莎1,2, 陈曦3, 王凤舞4, 徐亚平1,2, 叶桐1,2, 申丽1,2*
1. 扬州大学医学院 (转化医学研究院), 江苏 扬州 225001;
2. 江苏省中西医结合老年病防治重点实验室, 江苏 扬州 225001;
3. 扬州市中医院, 江苏 扬州 225001;
4. 青岛农业大学食品科学与工程学院, 山东 青岛 266109
摘要:
采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和高效液相色谱等方法从海带内生菌Alternaria sp.W-1发酵液粗浸膏中分离得到6个化合物,经高分辨质谱、核磁共振谱及文献比对的方法将它们鉴定为:6-iso-tricycloalter‐narene 6a (1)、tricycloalternarene 6a (2)、tricycloalternarene B (3)、uracil (4)、5-methyluracil (5)和lumichrome (6),其中,6-iso-tricycloalternarene 6a (1)是一个未见文献报道的新化合物。体外细胞毒活性测定显示,化合物13对人肝癌细胞株SMMC-7721和人胃癌细胞株SGC-7901有微弱的抑制活性。
关键词:    海带      内生菌      Alternaria sp.      次生代谢产物      tricycloalternarene化合物     
Secondary metabolites of endophyte Alternaria sp. W-1 from Laminaria japonica
WANG Sha-sha1,2, CHEN Xi3, WANG Feng-wu4, XU Ya-ping1,2, YE Tong1,2, SHEN Li1,2*
1. Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou 225001, China;
2. Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou 225001, China;
3. Yangzhou Hospital of TCM, Yangzhou 225001, China;
4. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
Abstract:
Six compounds were isolated from the crude extract of the liquid culture of Alternaria sp. W-1 by silica gel column chromatography, Sephadex LH-20 gel column chromatography, and HPLC. They were identified as 6-iso-tricycloalternarene 6a (1), tricycloalternarene 6a (2), tricycloalternarene B (3), uracil (4), 5-methyluracil (5), and lumichrome (6) through HR-MS, NMR and literature comparison. 6-iso-Tricycloalternarene 6a (1) is a new compound which has never been reported in the literature. In cytotoxicity assay, compounds 1-3 showed weak inhibition activity to human hepatoma cell line SMMC-7721 and human gastric cell line SGC-7901.
Key words:    Laminaria japonica    endophyte    Alternaria sp.    secondary metabolite    tricycloalternarene compound   
收稿日期: 2021-04-06
DOI: 10.16438/j.0513-4870.2021-0488
基金项目: 山东省自然科学基金面上项目(ZR2020MB101).
通讯作者: 申丽,Tel:86-514-87992233,E-mail:shenli@yzu.edu.cn
Email: shenli@yzu.edu.cn
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