药学学报, 2021, 56(10): 2830-2834
引用本文:
张彬, 曾国平, 熊校勤, 周巧巧. 侧蒿中一个新的桉烷型倍半萜及其肿瘤细胞毒活性[J]. 药学学报, 2021, 56(10): 2830-2834.
ZHANG Bin, ZENG Guo-ping, XIONG Xiao-qin, ZHOU Qiao-qiao. Tumour cytotoxic activities of a new eudesmane-type sesquiterpenoid from Artemisia deversa Diels[J]. Acta Pharmaceutica Sinica, 2021, 56(10): 2830-2834.

侧蒿中一个新的桉烷型倍半萜及其肿瘤细胞毒活性
张彬1,2*, 曾国平1,2, 熊校勤1,2, 周巧巧1,2
1. 湖北第二师范学院化学与生命科学学院, 湖北 武汉 430205;
2. 植物抗癌活性物质提纯与应用湖北省重点实验室, 湖北 武汉 430205
摘要:
采用溶剂提取法,Diaion HP-20大孔树脂、CHP20/P120 MCI、Sephadex LH-20葡聚糖凝胶、硅胶及制备高效液相等色谱方法对侧蒿的乙醇提取物进行分离纯化,得到5个化合物,根据质谱、核磁共振谱以及X-ray单晶衍射等数据确定了它们的结构,分别为侧蒿缩醛(1)、7-羟基-6-甲氧基香豆素(2)、6,7-二甲氧基香豆素(3)、咖啡酸(4)和4',5,7-三羟基黄酮(5)。其中,化合物1为新化合物。采用甲基噻唑蓝法(MTT)测试了化合物15对四种细胞株(人肝癌细胞HepG2、人非小细胞肺癌细胞A549、人宫颈癌细胞HeLa、人胚肺细胞MRC-5)的体外细胞毒活性,结果表明,化合物15对A549和HepG2显示出较强的生长抑制活性,IC50值分别为8.36、16.51 μmol·L-1和17.06、7.95 μmol·L-1
关键词:    侧蒿      化学成分      侧蒿缩醛      细胞毒活性     
Tumour cytotoxic activities of a new eudesmane-type sesquiterpenoid from Artemisia deversa Diels
ZHANG Bin1,2*, ZENG Guo-ping1,2, XIONG Xiao-qin1,2, ZHOU Qiao-qiao1,2
1. Department of Chemistry and Life Science, Hubei University of Education, Wuhan 430205, China;
2. Hubei Key Laboratory of Purification and Application of Plant Anti-cancer Active Ingredients, Wuhan 430205, China
Abstract:
Five compounds were isolated from an ethanol extract of Artemisia deversa Diels by solvent extraction, Diaion HP-20, CHP20/P120 MCI, Sephadex LH-20, silica gel and preparative high performance liquid chromatography. Their structures were elucidated by MS, NMR and X-ray as artemideversal (1), 7-hydroxy-6-methoxycoumarin (2), 6,7-dimethoxycoumarin (3), caffeic acid (4) and 4',5,7-trihydroxy flavone (5). Compound 1 is a new eudesmane-type sesquiterpene. In vitro cytotoxic activities of the five compounds were explored by MTT testing with HepG2, A549, HeLa and MRC-5 cell lines. Results show that compounds 1 and 5 significantly inhibited cellular proliferation. The IC50 of compound 1 in A549 and HepG2 cells was 8.36 and 16.51 μmol·L-1; the IC50 of compound 5 in A549 and HepG2 cells was 17.06 and 7.95 μmol·L-1.
Key words:    Artemisia deversa Diels    chemical composition    artemideversal    cytotoxic activity   
收稿日期: 2021-05-24
DOI: 10.16438/j.0513-4870.2021-0781
基金项目: 国家自然科学基金资助项目(81903115);湖北省教育厅科学技术研究项目(B2019183).
通讯作者: 张彬,Tel:86-27-87943931,E-mail:jaina96@163.com
Email: jaina96@163.com
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