药学学报, 2021, 56(10): 2858-2866
引用本文:
马鑫彤, 朱枚桦, 王南, 张馨月, 张庆宇, 王宇菲, 侯钰莹, 李志凌, 姚娜*, 刘秀明*. 红花类黄酮生物合成通路2-ODD基因家族鉴定与表达分析[J]. 药学学报, 2021, 56(10): 2858-2866.
MA Xin-tong, ZHU Mei-hua, WANG Nan, ZHANG Xin-yue, ZHANG Qing-yu, WANG Yu-fei, HOU Yu-ying, LI Zhi-ling, YAO Na*, LIU Xiu-ming*. Identification and expression analysis of 2-ODD gene family involved in flavonoid biosynthesis pathway in safflower[J]. Acta Pharmaceutica Sinica, 2021, 56(10): 2858-2866.

红花类黄酮生物合成通路2-ODD基因家族鉴定与表达分析
马鑫彤, 朱枚桦, 王南, 张馨月, 张庆宇, 王宇菲, 侯钰莹, 李志凌, 姚娜*, 刘秀明*
吉林农业大学生命科学学院, 生物反应器与药物开发教育部工程研究中心, 吉林 长春 130118
摘要:
黄烷酮3-羟化酶(F3H)、黄酮醇合成酶(FLS)和花青素合成酶(ANS)是植物2-酮戊二酸依赖性双加氧酶(2-ODD)超级家族中的成员,在植物类黄酮生物合成途径中起着重要的作用。本研究利用生物信息学的方法,从红花基因组数据库中共筛选鉴定16个类黄酮生物合成通路上的2-ODD基因,这些基因编码蛋白的长度为283(CtFLS2)~442 aa (CtANS6),分子质量为32 062.05(CtFLS2)~48 245.49 Da (CtANS6),其编码蛋白均为亲水性蛋白。系统进化将其分为3个亚家族,保守基序分析发现该家族成员均含有H-x-D-xn-H和R-x-S两个保守残基,对上游2 000 bp区域顺式作用元件分析显示该家族基因受到光、温度等环境因素及水杨酸、茉莉酸甲酯等植物激素的调控,利用qRT-PCR技术揭示了红花类黄酮生物合成通路上的2-ODD家族基因成员在红花不同花期及叶片中的差异表达。本研究可为深入挖掘红花类黄酮生物合成途径上的2-ODD基因的功能奠定基础。
关键词:    红花      类黄酮      2-酮戊二酸依赖性双加氧酶      生物信息学      表达分析     
Identification and expression analysis of 2-ODD gene family involved in flavonoid biosynthesis pathway in safflower
MA Xin-tong, ZHU Mei-hua, WANG Nan, ZHANG Xin-yue, ZHANG Qing-yu, WANG Yu-fei, HOU Yu-ying, LI Zhi-ling, YAO Na*, LIU Xiu-ming*
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, College of Life Science, Jilin Agricultural University, Changchun 130118, China
Abstract:
Flavanone 3-hydroxylase (F3H), flavonol synthase (FLS) and anthocyanin synthase (ANS) are members of the 2-oxoglutarate-dependent oxygenase (2-ODD) superfamily, which play important functions in plant flavonoid biosynthesis. In this study, 16 2-ODD genes involved in flavonoid biosynthesis pathway were screened and identified from safflower genome database using bioinformatics method. The length of the proteins encoded by these genes ranged from 283 (CtFLS2) to 442 aa (CtANS6), and the molecular weight ranged from 32 062.05 (CtFLS2) to 48 245.49 Da (CtANS6). The proteins encoded by these genes were hydrophilic. Phylogeny divided it into three subfamilies, two conserved residues, H-x-D-xn-H and R-x-S, were found in this gene family using conserved motifs analysis, the analysis of cis-acting elements in the upstream 2 000 bp region showed that these genes might be regulated by environmental factors such as light, temperature and plant hormones such as salicylic acid, methyl jasmonate and so on. The members of the safflower 2-ODD gene family involved in flavonoid biosynthesis pathway were analyzed by qRT-PCR, the result showed that the expression patterns of these members were different in diverse flowering period and leaf. This study can lay a foundation for further exploring the function of 2-ODD genes involved in flavonoid biosynthesis pathway in safflower.
Key words:    safflower    flavonoids    2-oxoglutarate-dependent oxygenase    bioinformatics    expression analysis   
收稿日期: 2021-04-27
DOI: 10.16438/j.0513-4870.2021-0633
基金项目: 国家自然科学基金项目(31771868);吉林省科技厅自然科学基金项目(20190201172JC,20190201175JC,20150623024TC-11);吉林农业大学大学生创新创业训练计划项目.
通讯作者: 姚娜,Tel:13174456545,E-mail:xiuming1211@163.com;刘秀明,Tel:18643129718,E-mail:yaona1103@126.com
Email: xiuming1211@163.com;yaona1103@126.com
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