药学学报, 2021, 56(11): 3097-3103
引用本文:
范锡玲, 刘晏灵, 曹彦刚, 任英杰, 王梦娜, 陈旭, 何晨, 郑晓珂, 冯卫生. 怀中1号鲜地黄化学成分研究[J]. 药学学报, 2021, 56(11): 3097-3103.
FAN Xi-ling, LIU Yan-ling, CAO Yan-gang, REN Ying-jie, WANG Meng-na, CHEN Xu, HE Chen, ZHENG Xiao-ke, FENG Wei-sheng. Chemical constituents of the fresh roots of Huaizhong NO.1 Rehmannia glutinosa[J]. Acta Pharmaceutica Sinica, 2021, 56(11): 3097-3103.

怀中1号鲜地黄化学成分研究
范锡玲1,2, 刘晏灵1,2, 曹彦刚1,2, 任英杰1,2, 王梦娜1,2, 陈旭1,2, 何晨1,2, 郑晓珂1,2, 冯卫生1,2*
1. 河南中医药大学药学院, 河南 郑州 450046;
2. 河南省中药开发工程技术研究中心, 河南 郑州 450046
摘要:
采用Toyopreal HW-40C、Sephadex LH-20、硅胶和半制备液相等多种色谱学技术从怀中1号鲜地黄中分离得到20个化合物。根据理化性质与波谱数据鉴定其结构,分别为3-methyl-2-(hydroxymethyl)-4H-pyrone-4-one (1)、3-氨基-2-吡啶乙醇 (2)、(5'S)-2-oxo-N-phenylpyrrolidine-3-carboxamide (3)、焦谷氨酸甲酯 (4)、吲唑 (5)、尿苷 (6)、腺苷 (7)、rehmanalkaloid C (8)、1-(3-乙基苯基)-1,2-乙二醇 (9)、(3-乙基苯基)-1,2-乙二醇 (10)、2-羟甲基苯基-1-O-β-D-葡萄糖苷 (11)、玉叶金花苷酸甲酯 (12)、11-methylforsythide (13)、7-去氧栀子新苷 (14)、1-O-α-L-鼠李糖苷 (1→6)-β-D-吡喃葡萄糖苷 (15)、6-deoxy-D-mannono-1,4-lactone (16)、富马酸单甲酯 (17)、daphneresinol (18)、二氢槲皮素 (19)、rhamnopyranosyl vaniloyl (20)。其中化合物1为新化合物,命名为3-methyl-2-(hydroxymethyl)-4H-pyrone-4-one,化合物23为新天然产物,化合物459111314以及1619是从地黄属中首次分离得到的。
关键词:    玄参科      鲜地黄      化学成分      3-methyl-2-(hydroxymethyl)-4H-pyrone-4-one      3-氨基-2-吡啶乙醇      (5'S)-2-oxo-N-phenylpyrrolidine-3-carboxamide     
Chemical constituents of the fresh roots of Huaizhong NO.1 Rehmannia glutinosa
FAN Xi-ling1,2, LIU Yan-ling1,2, CAO Yan-gang1,2, REN Ying-jie1,2, WANG Meng-na1,2, CHEN Xu1,2, HE Chen1,2, ZHENG Xiao-ke1,2, FENG Wei-sheng1,2*
1. School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China;
2. The Engineering and Technology Center for Chinese Medicine Development of Henan Province China, Zhengzhou 450046, China
Abstract:
Twenty compounds were isolated and purified from the fresh roots of Huaizhong NO.1 Rehmannia glutinosa by various chromatographic techniques such as Toyopreal HW-40C, Sephadex LH-20, silica gel and semi-preparative HPLC. Their structures were identified by analysis of physicochemical properties and spectral data, and determined as 3-methyl-2-(hydroxymethyl)-4H-pyrone-4-one (1), 3-amino-2-pyridine ethanol (2), (5'S)-2-oxo-N-phenylpyrrolidine-3-carboxamide (3), L-pyroglutamic acid methyl ester (4), indazole (5), uridine (6), adenosine (7), rehmanalkaloid C (8), 1-(3-ethylphenyl)-1,2-ethanediol (9), (3-ethylphenyl)-1,2-ethanediol (10), 2-hydroxymethylphenyl-1-O-β-D-glucopyranoside (11), mussaenoside (12), 11-methylforsythide (13), 7-deoxygardoside (14), 1-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside (15), 6-deoxy-D-mannono-1,4-lactone (16), monoethylfumarate (17), daphneresinol (18), dihydroquercetin (19), rhamnopyranosyl vaniloyl (20). Compound 1 is a new compound, named as 3-methyl-2-(hydroxymethyl)-4H-pyrone-4-one, compounds 2 and 3 are new natural products, and compounds 4, 5, 9-11, 13, 14 and 16-19 are isolated from Rehmannia for the first time.
Key words:    Scrophulariaceae    fresh Rehmannia glutinosa    chemical component    3-methyl-2-(hydroxymethyl)-4H-pyrone-4-one    3-amino-2-pyridine ethanol    (5'S)-2-oxo-N-phenylpyrrolidine-3-carboxamide   
收稿日期: 2021-05-24
DOI: 10.16438/j.0513-4870.2021-0777
基金项目: 国家重点研发计划:中医药现代化研究重点专项-地黄特色中药材产业链关键技术研究(2017YFC1702800);河南省重大科技专项:地黄、山药、牛膝豫产道地药材品质保障关键技术与品质特色研究(171100310500).
通讯作者: 冯卫生,Tel/Fax:86-371-60190296,E-mail:fwsh@hactcm.edu.cn
Email: fwsh@hactcm.edu.cn
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