药学学报, 2021, 56(11): 3112-3117
引用本文:
杨雯, 于海艳, 王栋, 周雪梅, 张建平, 高磊. 肉苁蓉类药材多糖提取工艺优化及多糖的平均分子质量分布[J]. 药学学报, 2021, 56(11): 3112-3117.
YANG Wen, YU Hai-yan, WANG Dong, ZHOU Xue-mei, ZHANG Jian-ping, GAO Lei. Optimization of the extraction process for polysaccharides from Cistanche and their molecular weight distribution[J]. Acta Pharmaceutica Sinica, 2021, 56(11): 3112-3117.

肉苁蓉类药材多糖提取工艺优化及多糖的平均分子质量分布
杨雯1, 于海艳1, 王栋2, 周雪梅2, 张建平2, 高磊3*
1. 内蒙古医科大学药学院, 内蒙古 呼和浩特 010000;
2. 内蒙古自治区药品检验研究院, 内蒙古自治区中蒙药标准研究重点实验室, 内蒙古 呼和浩特 010000;
3. 内蒙古自治区药物警戒中心, 内蒙古 呼和浩特 010000
摘要:
本文以肉苁蓉多糖的得率以及质量分数为评价指标,通过单因素考察以及正交试验确定复合酶联合超声提取工艺的最适提取条件,从而得到一种简便、高效提取肉苁蓉多糖的方法。用此方法提取荒漠肉苁蓉、管花肉苁蓉、盐生肉苁蓉、沙苁蓉中的多糖,通过高分子排阻色谱-十八角度激光光散射仪配置示差检测器测定4种肉苁蓉多糖的平均分子质量、分布以及多糖的质量分数,分析4种肉苁蓉多糖间存在的差异。最终确定提取肉苁蓉多糖的最佳工艺参数为复合酶用量4%、复合酶配比 (纤维素酶∶果胶酶) 1∶3、超声功率350 W (40 kHz)、超声时间20 min、酶解温度50 ℃、pH值为6。在此提取工艺下重复提取3次平均得率为5.14%,质量分数为90.78%,综合指标为47.96%,RSD为1.51%,重现性较好。通过软件计算可知4种肉苁蓉类多糖均为球形结构,但其平均分子质量及其分布存在差异,优化后的复合酶联合超声提取工艺能够有效地增大肉苁蓉多糖的得率,是一种简单方便、经济环保的提取方法,为日后多糖的研究奠定了基础。
关键词:    肉苁蓉多糖      复合酶      平均分子质量     
Optimization of the extraction process for polysaccharides from Cistanche and their molecular weight distribution
YANG Wen1, YU Hai-yan1, WANG Dong2, ZHOU Xue-mei2, ZHANG Jian-ping2, GAO Lei3*
1. School of Pharmacy, Inner Mongolia Medical University, Hohhot 010000, China;
2. Inner Mongolia Key Laboratory of Chinese and Mongolian Medicines Standard Research, Inner Mongolia Pharmaceutical Inspection Institute, Hohhot 010000, China;
3. Inner Mongolia Pharmacovigilance Center, Hohhot 010000, China
Abstract:
A simple and efficient method for extracting Cistanche polysaccharides was obtained and used to extract four Cistanche polysaccharides. The molecular weight, distribution and polysaccharide mass fraction of the four Cistanche polysaccharides were measured by High performance size exclusion chromatography-multiangle laser light scatter-refractive index detector (HPSEC-MALLS-RID) and differences between the four Cistanche polysaccharides were determined. The results of a single factor test and an orthogonal test showed that the best process parameters for extracting Cistanche polysaccharides used a mixture of enzyme (4%), a ratio of complex enzyme (cellulase∶pectinase) 1∶1, ultrasonic power at 350 W (40 kHz), for 20 min, enzymolysis temperature of 50 ℃, and pH 6. The average yield of three repeated extractions was 5.14%, the mass fraction was 90.78%, the comprehensive index was 47.96%, and the RSD = 1.51%, indicating good reproducibility. The four Bossica Cistanche polysaccharides are all spherical structures but their molecular weights and distributions differ. The optimized compounded enzyme combined with ultrasonic extraction increases the yield of Cistanche polysaccharides. It is a simple, convenient, economical and environmentally friendly extraction method, which lays the foundation for future polysaccharide research.
Key words:    Cistanche deserticola polysaccharide    complex enzyme    molecular weight   
收稿日期: 2021-06-23
DOI: 10.16438/j.0513-4870.2021-0266
基金项目: 内蒙古自治区科技计划项目(2019GG132).
通讯作者: 高磊,Tel:13848919268,E-mail:nmyjsgl@sina.com
Email: nmyjsgl@sina.com
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