药学学报, 2021, 56(11): 3141-3152
引用本文:
徐境荣, 于钦川, 赖莉, 王淑美, 罗国安, 谢媛媛. 基于多指标成分含量测定探索黄芩药材质量综合评价模式[J]. 药学学报, 2021, 56(11): 3141-3152.
XU Jing-rong, YU Qin-chuan, LAI Li, WANG Shu-mei, LUO Guo-an, XIE Yuan-yuan. Comprehensive quality evaluation of Scutellaria baicalensis Georgi by determination of multi-component content[J]. Acta Pharmaceutica Sinica, 2021, 56(11): 3141-3152.

基于多指标成分含量测定探索黄芩药材质量综合评价模式
徐境荣1,2,3, 于钦川1,2,3, 赖莉1,2,3, 王淑美1,2,3, 罗国安4, 谢媛媛1,2,3*
1. 广东药科大学中药学院, 广东 广州 510006;
2. 广东省中药质量工程技术研究中心, 广东 广州 510006;
3. 国家中医药管理局中药数字化质量评价技术重点研究室, 广东 广州 510006;
4. 清华大学化学系, 北京 100084
摘要:
本研究基于多指标成分含量测定探索黄芩药材质量综合评价模式,采用高效液相色谱 (HPLC) 建立黄芩中8种黄酮类成分同时定量分析的方法,建立以黄芩苷为内参比物质的一测多评法 (QAMS);采用2,2'-联氨-双 (3-乙基苯并噻唑啉-6-磺酸) 二胺盐 (ABTS+) 法测定不同批次黄芩药材抗氧化活性,并以这8种成分的含量与抗氧化活性效价进行灰色关联度分析,评价不同成分对黄芩药材抗氧化活性的贡献;比较不同产地、不同商品规格、不同采收年限黄芩药材的综合质量。结果表明,以黄芩苷为内参比物质测得的各指标成分相对校正因子系统适应性良好,QAMS法测定结果与外标法无显著差异;黄芩苷等8种指标成分与黄芩药材抗氧化活性关联性较强,灰色关联系数在0.772 4~0.808 6之间,其中黄芩苷、汉黄芩苷和粘毛黄芩素III等的含量与药材抗氧化活性负相关,而黄芩素、汉黄芩素、千层纸素A、去甲汉黄芩素苷和千层纸素A-7-O-β-D葡萄糖醛酸苷等的含量则与药材抗氧化活性正相关。黄酮苷与黄酮苷元含量比值 (G/A) 结合药材性状特征可作为划分不同品类黄芩药材的评价指标。G/A小于10为枯芩,G/A大于10为子芩或含有枯心的子芩。本研究所建立的QAMS简便、准确,可用于黄芩8种黄酮类成分含量测定,结合ABTS+抗氧化活性与灰色关联度分析法建立了基于生物活性的黄芩质量综合评价模式,为建立符合中药自身特色的质量标准提供了新的思路和方法。
关键词:    黄芩      黄芩苷      一测多评      相对校正因子      抗氧化      灰色关联度分析     
Comprehensive quality evaluation of Scutellaria baicalensis Georgi by determination of multi-component content
XU Jing-rong1,2,3, YU Qin-chuan1,2,3, LAI Li1,2,3, WANG Shu-mei1,2,3, LUO Guo-an4, XIE Yuan-yuan1,2,3*
1. College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;
2. Guangdong Research Center for Quality Engineering Technology of Traditional Chinese Medicine, Guangzhou 510006, China;
3. Key Laboratory of Digitalized Quality Evaluation Technology of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, Guangzhou 510006, China;
4. Department of Chemistry, Tsinghua University, Beijing 100084, China
Abstract:
A model for quality evaluation was developed by determination of multi-index components in Scutellaria baicalensis Georgi. Eight flavonoids in Scutellaria baicalensis Georgi were quantified by high performance liquid chromatography (HPLC), and a quantitative analysis of multi-components with a single marker (QAMS) method with baicalin as the internal reference substance was established. The 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS+) method was applied to determine the antioxidant activity of different batches of Scutellaria baicalensis Georgi. Grey relevance analysis was conducted to calculate the grey correlation coefficients of each antioxidant in the herb. The holistic quality of 75 batches of collected samples from different product regions was evaluated to distinguish different commercial types of Scutellaria baicalensis Georgi. The relative correction factor (RCF) of each index component with baicalin as the internal reference indicated good applicability, and there was no significant difference in assay results between the QAMS method and the external standard method (ESM). The results of grey correlation analysis showed that the content of eight index components including baicalin correlated strongly with the antioxidant activity of Scutellaria baicalensis Georgi. The calculated grey relational coefficient ranged from 0.772 4 to 0.808 6. The content of oroxylin A-7-O-β-D-glucuronide, norwogonin-7-O-β-D-glucuronide, baicalein, wogonin and oroxylin A showed positive correlations with the antioxidant potency. In contrast, baicalin, wogonoside and viscidulin III content exhibited negative correlations. The ratio of the flavonoid glycosides and flavonoid glycoside aglycones were found to be a critical factor to distinguish the different types of commercial herbs. The crude herbs could be characterized as Scutellaria baicalensis pith-decayed with a G/A ratio less than 10, and the ratio in Scutellaria baicalensis pith-not decayed should be more than 10. The QAMS method established in this study is simple and accurate, and can be used for the determination of eight flavonoids in Scutellaria baicalensis Georgi. Combined with ABTS+ antioxidant activity and grey correlation analysis, a comprehensive model for Scutellaria baicalensis Georgi quality determination based on biological activity was established, which provides new ideas and methods for the establishment of quality standards consistent with the characteristics of traditional Chinese medicine.
Key words:    Scutellaria baicalensis Georgi    baicalin    quantitative analysis of multi-components with a single marker    relative correlation factor    antioxidant    grey correlation analysis   
收稿日期: 2021-06-28
DOI: 10.16438/j.0513-4870.2021-0955
基金项目: 国家重点研发计划“中医药现代化研究”重点专项课题资助(2017YFC1700800).
通讯作者: 谢媛媛,Tel:13902294350,E-mail:yuanyuan8078@163.com
Email: yuanyuan8078@163.com
相关功能
PDF(2414KB) Free
打印本文
0
作者相关文章
徐境荣  在本刊中的所有文章
于钦川  在本刊中的所有文章
赖莉  在本刊中的所有文章
王淑美  在本刊中的所有文章
罗国安  在本刊中的所有文章
谢媛媛  在本刊中的所有文章

参考文献:
[1] Zhou X, Fu L, Wang PL, et al. Drug-herb interactions between Scutellaria baicalensis and pharmaceutical drugs: Insights from experimental studies, mechanistic actions to clinical applications[J]. Biomed Pharmacother, 2021, 138: 111445.
[2] Bai QY, Tao SM, Tian JH, et al. Progress of research on effect and mechanism of Scutellariae Radix on preventing liver diseases[J]. China J Chin Mater Med (中国中药杂志), 2020, 45: 2808-2816.
[3] Zhao TT, Tang HL, Xie L, et al. Scutellaria baicalensis Georgi. (Lamiaceae): a review of its traditional uses, botany, phytochemistry, pharmacology and toxicology[J]. J Pharm Pharmacol, 2019, 71: 1353-1369.
[4] Zheng YF, Wang JJ, Fu CM, et al. Progress of research on chemical constituents and pharmacological effects of Scutellaria baicalensis[J]. Chin Tradit Pat Med (中成药), 2016, 38: 141-147.
[5] Liu JX, Meng FY, Zhang SH, et al. Simultaneous determination of baicalin, wogonoside, baicalein, wogonin, and oroxylin-A in Scutellaria baicalensis by UPLC[J]. Chin Tradit Herb Drugs (中草药), 2014, 45: 1477-1480.
[6] Bai CK, Yang JJ, Cao B, et al. Growth years and post-harvest processing methods have critical roles on the contents of medicinal active ingredients of Scutellaria baicalensis[J]. Ind Crop Prod, 2020, 158: 112985.
[7] Wang D, Jiang YJ, Liang Y, et al. Identification of genuine and non-genuine medicinal materials of Scutellaria baicalensis based on HPLC fingerprint[J]. China J Chin Mater Med (中国中药杂志), 2013, 38: 1951-1960.
[8] Pang YY, Xue LY, Zheng YH, et al. Comparatice study on quality of Scutellaria baicalensis Georgi in different harvest periods using UHPLC-MS/MS metabolomics technology[J]. Acta Pharm Sin (药学学报), 2017, 52: 1903-1909.
[9] Li XD, Gu XZ, Cheng LP, et al. Investigation on the market situation of specifications and grading of Scutellaria baicalensis slices and discussion of relevant ancient and modern literature[J]. J Chin Med Mater (中药材), 2014, 37: 150-152.
[10] Zhang FL, Zhao JW, Hu JH, et al. Evaluation of different effect of pithnodecayed and pith-decayed products of Scutellariae Radix on large intestine damp - heat syndrome based on pharmacological indexes[J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志), 2020, 26: 57-64.
[11] Wang L, Luo FZ, Chen WS. Overview of Kuqin and Ziqin[J]. China's Naturopathy (中国民间疗法), 2020, 28: 115-119.
[12] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (中华人民共和国药典)[S]. 2020 Ed. Part I. Beijing: China Medical Science Press, 2020: 314.
[13] Liu T, Kong M, Liu LF, et al. Correlation research between the product specification and intrinsic quality of Scutellaria baicalensis Georgi[J]. Acta Chin Med Pharmacol, 2018, 46: 41-47.
[14] Wang D, Jiang YJ, Liang Y, et al. Study on HPLC-fingerprint-based identification of Dao-di herb and non Dao-di herb of Scutellariae Radix[J]. China J Chin Mater Med (中国中药杂志), 2012, 37: 426-433.
[15] Yang ZW, Xu F, Liu X, et al. An untargeted metabolomics approach to determine component differences and variation in their in vivo distribution between Kuqin and Ziqin, two commercial specifications of Scutellaria Radix[J]. RSC Adv, 2017, 7: 54682-54695.
[16] Chen QY, Wang CQ, Yang ZW, et al. Difference in anti-inflammatory activity of two kinds of Scutellaria baicalensis in LPS-induced macrophages[C]//Proceeding of the 2016 Chinese Pharmaceutical Association Annual Symposium (2016年中国药学大会暨第十六届中国药师周论文集). Beijing: Chinese Pharmaceutical Association, 2016: 3.
[17] Zhao JW. Study on the Difference of Chemical Composition and Pharmacodynamics between Scutellaria baicalensis Pith-nodecayed and Scutellaria baicalensis Pith-decayed (子芩与枯芩化学成分及药效差异研究)[D]. Wuhan: Hubei University of Traditional Chinese Medicine, 2019.
[18] Lu J, Shi SM. Green analytical chemistry and the testing method of traditional Chinese medicine[J]. Chin J Pharm Anal (药物分析), 2019, 39: 580-587.
[19] Gao HM, Song ZH, Wang ZM, et al. Overview on quantitative analysis of multi-components by single-marker[J]. China J Chin Mater Med (中国中药杂志), 2012, 37: 405-416.
[20] Wang ZM, Gao HM, Fu XT, et al. Multi-component quantitation by one marker new method for quality evaluation of Chinese herbal medicine[J]. China J Chin Mater Med (中国中药杂志), 2006, 31: 1925-1928.
[21] Luo ZL, Qiu F, Wei RW, et al. Application of relative correction factor in multi-index determination of Chinese materia medica[J]. Chin Tradit Herb Drugs (中草药), 2012, 43: 1448-1452.
[22] Yang Y, Huang LY, Zhu ML, et al. Quantitative analysis of multi-components with single-marker in China Pharmacopoeia (2015 Edition)[J]. Cent South Pharm (中南药学), 2017, 15: 1738-1741.
[23] Zuo L, Meng SN. Advances in the application of quantitative analysis of multi-components with single-marker in the analysis of traditional Chinese medicines[J]. J Chin Pharm (中国药房), 2016, 27: 2589-2592.
[24] Yan CP, Wu Y, Weng ZB, et al. Development of an HPLC method for absolute quantification and QAMS of flavonoids components in Psoralea corylifolia L.[J]. J Anal Methods Chem, 2015. DOI: 10.1155/2015/792637.
[25] Lou SH, Xie YY, Wang YM, et al. Application of internal standard correction method in multi-component quantitative analysis of Fructus Gardeniae[J]. Lishizhen Med Mater Med Res (时珍国医国药), 2012, 23: 1812-1814.
[26] Zhu H, Qiu YN, Gong DD, et al. A novel method for quality consistency evaluation of Yankening Tablet by multi-wavelength fusion profiling combined with overall components dissolution method and antioxidant activity analysis[J]. J Pharm Biomed Anal, 2021, 196: 113910.
[27] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (中华人民共和国药典)[S]. 2020 Ed. Part I. Beijing: China Medical Science Press, 2020: 77, 280, 316.
[28] Liao HF, Ye J, Gao LL, et al. The main bioactive compounds of Scutellaria baicalensis Georgi for alleviation of inflammatory cytokines: a comprehensive review[J]. Biomed Pharmacother, 2021, 133: 110917.
[29] Olena V, Liudmyla S, Olga G, et al. Antioxidant capacity of plant raw material of Scutellaria baicalensis Georgi[J]. Potravinarstvo, 2019, 13: 614-621.
[30] Kong BY, Wei LB, Guo QL. Progress in antitumor activity of baicalin[J]. Acta Pharm Sin (药学学报), 2021, 56: 1537-1543.
[31] Chang YF, Gong WX, Zheng YH, et al. Urinary metabolomics study of the effects of Scutellaria baicalensis Georgi ethanol extract on D-galactose-induced rats[J]. Acta Pharm Sin (药学学报), 2016, 51: 86-92.
[32] Zeng WC, Shi B. Common methods of antioxidant activity evaluation for natural products: a review[J]. Chem Ind Eng Prog (化工进展), 2013, 32: 1205-1213, 1247.
[33] Kalili KM, De SS, Van HT, et al. Comprehensive two-dimensional liquid chromatography coupled to the ABTS radical scavenging assay: a powerful method for the analysis of phenolic antioxidants[J]. Anal Bioanal Chem, 2014, 406: 4233-4242.
[34] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China (中华人民共和国药典)[S]. 2020 Ed. Part I. Beijing: China Medical Science Press, 2020: 206.
[35] Morimoto S, Harioka T, Shoyama Y. Purification and characterization of flavone-specific β-glucuronidase from callus cultures of Scutellaria baicalensis Georgi[J]. Planta, 1995, 195: 535-540.
[36] Li ZZ, Ding GR, Geng JT, et al. Revision of the Sign of Materia Medica (本草徴要重订)[M]. Beijing: Beijing Science and Technology Press, 1986.
相关文献:
1.周明皓, 张敏, 张硕, 王鹏娇, 孟小夏, 曹思源, 杨七妹, 高秀丽.不同相对分子质量的聚乙二醇对黄芩苷药代动力学的影响[J]. 药学学报, 2021,56(5): 1416-1423
2.孔邦彦, 魏立彬, 郭青龙*.黄芩苷的抗肿瘤作用研究进展[J]. 药学学报, 2021,56(6): 1537-1543
3.姚静, 孙欣光, 董蓉, 谢建宏, 王玉龙, 杨晓宁.HPLC-CAD一测多评法同时测定黄芪中6种成分含量[J]. 药学学报, 2021,56(2): 557-564
4.陈红珊, 杨玉琴, 张昊, 王鹏龙, 雷海民.基于分子热力学特征探讨中药黄芩的归经特性[J]. 药学学报, 2020,55(2): 330-334
5.宋佳, 庞溢媛, 高丽, 秦雪梅, 杜冠华, 周玉枝.基于血清代谢组学的黄芩花干预D-半乳糖致衰老大鼠作用研究[J]. 药学学报, 2019,54(3): 533-539
6.张影, 许华容, 李军山, 高晗, 毕开顺, 李清.基于一测多评法的茵陈标准汤剂中8种成分同时定量研究[J]. 药学学报, 2019,54(9): 1661-1666
7.刘雪松, 张丝雨, 赵曼茜, 王钧, 李页瑞, 代军, 滕传震, 柯潇, 陈勇, 吴永江.近红外光谱结合不同变量筛选方法用于黄芩提取过程中黄芩苷含量预测[J]. 药学学报, 2019,54(1): 138-143
8.王俊俊, 张俐, 郭青, 寇俊萍, 余伯阳, 谷丹华.一测多评法测定8个银翘解毒系列制剂中的7种酚酸类成分的含量[J]. 药学学报, 2015,50(4): 480-485
9.郭明, 伍周玲, 王春歌, 高小艳.黄芩苷-金属配合物的合成及其抗肿瘤活性研究[J]. 药学学报, 2014,49(3): 337-345
10.祝婧云 梁新丽 王光发 赵国巍 廖正根 曹运朝 陈绪龙 杨 明.白芷提取物对黄芩活性成分黄芩苷的吸收促进作用[J]. 药学学报, 2011,46(2): 232-237
11.陈红艳 耿淼 胡亚卓 王建华.黄芩苷通过上调SIRT1保护SH-SY5Y氧化应激的损伤[J]. 药学学报, 2011,46(9): 1039-1044
12.吴品江 许润春 苏柘僮 魏 萍 林彦君 杨 明 郑 琴.黄芩苷脂质体、β-环糊精包合物及磷脂复合物鼻黏膜渗透性及毒性研究[J]. 药学学报, 2009,44(4): 417-424
13.罗奇志 罗佳波 王有志.高效液相色谱-电喷雾串联四极杆质谱定性分析双黄连粉针中化学成分及其药味归属[J]. 药学学报, 2009,44(12): 1391-1396
14.刘兆明;马越鸣;王天明;郭忻.黄芩苷与黄芩素在离体大鼠肝、肾、小肠及膀胱中代谢的相互转化[J]. 药学学报, 2008,43(6): 664-668
15.朱晶晶;王智民;匡艳辉;张启伟;高其品;马妮.一测多评法同步测定人参和三七药材中多种人参皂苷的含量[J]. 药学学报, 2008,43(12): 1211-1216
16.居文政;刘芳;吴婷;夏小燕;谈恒山;王醒;熊宁宁.UPLC-MS/MS法同时测定人血浆中黄芩苷和绿原酸[J]. 药学学报, 2007,42(10): 1074-1077
17.冯有龙;余伯阳;董小平.高效液相色谱法同时测定三黄片中的蒽醌类、黄酮类及生物碱类化合物[J]. 药学学报, 2006,41(3): 285-288
18.刘萍;王菊英;李倩;许复郁;王姿颖;徐红岩;刘兆平;张岫美.黄芩苷对大鼠脑缺血再灌注损伤后海马神经元HSP70表达的影响[J]. 药学学报, 2006,41(7): 619-624
19.董庆华;郑树;;徐荣臻;吕庆华.黄芩苷元选择性诱导人白血病K562细胞凋亡[J]. 药学学报, 2003,38(11): 817-820
20.王新宏;安睿;邹云;张艺;王智华;洪筱坤.双黄连片的多组分质量分析[J]. 药学学报, 2001,36(12): 917-920
21.侯艳宁;朱秀媛;程桂芳.黄芩苷的抗炎机理[J]. 药学学报, 2000,35(3): 161-164
22.侯艳宁;程桂芳;朱秀媛.黄芩苷对小鼠肝细胞色素P450的选择性诱导[J]. 药学学报, 2000,35(12): 890-892
23.程智勇;韩凤梅;蔡敏;陈勇.HPCE法测定双黄连口服液中的黄芩苷和绿原酸[J]. 药学学报, 1999,34(11): 854-857