药学学报, 2013, 48(12): 1785-1791
引用本文:
吴淑娟, 王任烨, 薛继雄, 潘建春. 胡椒碱对肠易激综合征模型大鼠脑-肠5-羟色胺和突触小泡蛋白的影响[J]. 药学学报, 2013, 48(12): 1785-1791.
WU Shu-juan, WANG Ren-ye, XUE Ji-xiong, PAN Jian-chun. Effect of piperine on 5-HT and synaptophysin expression of rats with irritable bowel syndrome[J]. Acta Pharmaceutica Sinica, 2013, 48(12): 1785-1791.

胡椒碱对肠易激综合征模型大鼠脑-肠5-羟色胺和突触小泡蛋白的影响
吴淑娟1, 王任烨2, 薛继雄3, 潘建春1
1. 温州医科大学, 浙江 温州 325000;
2. 台州市食品药品检验所, 浙江 台州 318000;
3. 温州市中西医结合医院, 浙江 温州 325000
摘要:
探讨胡椒碱对慢急性联合应激大鼠的抑郁样行为学和内脏敏感性的改善作用以及对脑-肠中5-羟色胺(5-HT)和突触小泡蛋白表达的影响。42只SD大鼠随机分为正常对照组、模型组、胡椒碱(12.5、25、50及100 mg·kg-1)组和阳性对照组(丙咪嗪10 mg·kg-1)。采用慢急性联合应激建立肠易激综合征(IBS)模型来评估动物抑郁样行为和肠道敏感性的变化,用高效液相色谱法(HPLC)测定药物对大鼠海马和结肠5-HT含量的影响,用Western blotting法测定大鼠海马和结肠突触小泡蛋白的含量。与正常对照组相比,IBS模型组大鼠强迫游泳不动时间明显增加,糖水消耗量明显降低,大鼠内脏敏感性增加(P<0.05,P<0.01);大鼠海马中5-HT的水平下降,5-羟吲哚乙酸(5-HIAA)与5-HT的比例(5-HIAA/5-HT)增加(P<0.01),而结肠中5-HT的水平升高,5-HIAA与5-HT的比例(5-HIAA/5-HT)降低(P<0.05);大鼠海马中突触小泡蛋白的表达水平下降,结肠中表达水平反而增加(P<0.05)。胡椒碱能逆转上述引起的变化。IBS大鼠存在抑郁状态和内脏敏感性改变,脑-肠内5-HT和突触小泡蛋白表达异常,胡椒碱可改善IBS大鼠模型行为学变化,并调节5-HT和突触小泡蛋白的表达。
关键词:    胡椒碱      肠易激综合征      5-羟色胺      突触小泡蛋白     
Effect of piperine on 5-HT and synaptophysin expression of rats with irritable bowel syndrome
WU Shu-juan1, WANG Ren-ye2, XUE Ji-xiong3, PAN Jian-chun1
1. Wenzhou Medical University, Wenzhou 325000, China;
2. Taizhou Institute for Food and Drug Control, Taizhou 318000, China;
3. Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou 325000, China
Abstract:
This study is to explore the amelioration of piperine on chronic acute combining stress rat with depression-like behavior, visceral sensitivity, and its effect on the expression of serotonin (5-HT) and synaptophysin. Forty two SD rats were divided into seven groups: blank group, model group, piperine (12.5, 25, 50 and 100 mg·kg-1, ig) and imipramine (10 mg·kg-1, ip) groups. The rat model of irritable bowel syndrome was established by chronic acute combining stress, and then to evaluate depression-like behavior and visceral sensitivity. The expressions of 5-HT and synaptophysin in the hippocampus and colon were determined by high performance liquid chromatography (HPLC) and Western blotting, respectively. The duration of immobility of IBS rat in the forced swimming test had been significantly increased, the sucrose consumption of IBS rat had been reduced and visceral sensitivity was obviously elevated in the IBS model group as compared with those in the normal control group (P<0.05, P<0.01). As compared with those in the normal control group, the expression of 5-HT significantly decreased, 5-HIAA/5-HT ratio significantly increased in the hippocampus of IBS model group (P<0.05), but opposite presentations were noted in the colon (P<0.05). As compared with that in the normal control group, the synaptophysin expression in the hippocampus decreased significantly but obviously increased in the colon (P<0.05). Piperine improved the behavior of IBS rats, and reversed the levels of 5-HT and 5-HIAA, and 5-HIAA/5-HT proportion in the hippocampus and colon (P<0.05); besides, they significantly reverse the synaptophysin level in the hippocampus and colon (P<0.05). The presence of depression and visceral sensitivity had been changed in IBS rats, with abnormal expression of 5-HT and synaptophysin in the brain-gut system. Piperine can ameliorate the changes of the behavior and regulation of serotonin and synaptophysin expression in IBS rat model.
Key words:    piperine    irritable bowel syndrome    5-HT    synaptophysin   
收稿日期: 2013-08-01
基金项目: 温州市科技计划项目(Y20100201);瑞安市科技计划项目(201002063).
通讯作者: 潘建春
Email: wenzhoupan2003@163.com
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