药学学报, 2013, 48(12): 1807-1811
引用本文:
李俊贤, 王嘉毅, 张乐乐, 鄢丹, 王睿林, 李宝才, 肖小河. 微量量热法研究中药黄连6种生物碱之间相互作用关系[J]. 药学学报, 2013, 48(12): 1807-1811.
LI Jun-xian, WANG Jia-yi, ZHANG Le-le, YAN Dan, WANG Rui-lin, LI Bao-cai, XIAO Xiao-he. Microcalorimetric investigation on the interaction of six alkaloids from Rhizoma Coptidis[J]. Acta Pharmaceutica Sinica, 2013, 48(12): 1807-1811.

微量量热法研究中药黄连6种生物碱之间相互作用关系
李俊贤1,2, 王嘉毅1,3, 张乐乐1, 鄢丹1, 王睿林1, 李宝才2, 肖小河1
1. 解放军第302医院全军中药研究所, 北京 100039;
2. 昆明理工大学生命科学与技术学院, 云南 昆明 650093;
3. 江西中医药大学药学院, 江西 南昌 330004
摘要:
本文采用微量量热法研究黄连6种生物碱对痢疾杆菌生长代谢的影响,比较各生物碱在黄连抑菌效果中所占的贡献度,确定主要的药效物质,并围绕其展开与其他生物碱之间相互作用关系的研究。结果表明,0.8 mg·mL-1黄连水提液中,各生物碱的贡献度分别是:药根碱和非洲防己碱3.21%,表小檗碱2.49%,巴马汀4.27%,黄连碱36.31%,小檗碱52.83%,由此判断小檗碱与黄连碱为主要的抑菌活性物质。各成分相互作用关系显示,黄连中存在与抑菌活性相拮抗的物质;小檗碱与黄连碱之间存在协同作用;其他4种生物碱与小檗碱和黄连碱存在叠加作用。该结论为中药黄连质量标准的制定和新的组分中药的研制提供了参考。
关键词:    微量量热法      药效物质      黄连      痢疾杆菌      药物相互作用     
Microcalorimetric investigation on the interaction of six alkaloids from Rhizoma Coptidis
LI Jun-xian1,2, WANG Jia-yi1,3, ZHANG Le-le1, YAN Dan1, WANG Rui-lin1, LI Bao-cai2, XIAO Xiao-he1
1. Military Institute of Chinese Materia Medica, 302th Military Hospital of China, Beijing 100039, China;
2. College of Life Science and Technology, Kunming University of Science and Technology, Kunming 650093, China;
3. College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
Abstract:
How to identify active constituents of traditional Chinese medicines (TCMs) and study their interactions are key problems in the development of TCMs. The inhibitory effect of six alkaloids from Rhizoma Coptidis (RC) on Shigella dysenteriae (S. dysenteria) growth had been investigated by microcalorimetry in this study. Main active constituents of RC were confirmed by comparing their contributions to the bacteriostatic effect, and the interactions among active constituents were further researched. According to the result, in 0.8 mg·mL-1 extract of RC, the contributions of six active alkaloids including berberine, coptisine, epiberberine, palmatine and the combination of jatrorrhizine and columbamine were 52.83%, 36.31%, 2.49%, 4.27% and 3.21%, respectively. Therefore, berberine and coptisine were the main active constituents of RC that inhibited the growth of S. dysenteria. The study of interactions among the six alkaloids indicated that, ① there were some constituents antagonizing the inhibitory effect of RC, ② there was a synergy effect between berberine and coptisine, ③ there were additive effects between other four alkaloids and the main active constituents. These results may provide some useful references for the establishment of the quality standard for RC and the development of multi-component TCMs.
Key words:    microcalorimetry    active constituent    Rhizoma coptidis    Shigella dysenteriae    drug interaction   
收稿日期: 2013-04-30
基金项目: 国家自然科学基金资助项目(81274078,81322052,81303222).
通讯作者: 鄢丹, 肖小河
Email: yd277@126.com;pharmsci@126.com
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