药学学报, 2013, 48(12): 1817-1822
引用本文:
张蔚, 江曙, 钱大玮, 尚尔鑫, 钱叶飞, 任浩, 管汉亮, 段金廒. 肠道细菌对柚皮苷的代谢研究[J]. 药学学报, 2013, 48(12): 1817-1822.
ZHANG Wei, JIANG Shu, QIAN Da-wei, SHANG Er-xin, QIAN Ye-fei, REN Hao, GUAN Han-liang, DUAN Jin-ao. Metabolism of naringin produced by intestinal bacteria[J]. Acta Pharmaceutica Sinica, 2013, 48(12): 1817-1822.

肠道细菌对柚皮苷的代谢研究
张蔚, 江曙, 钱大玮, 尚尔鑫, 钱叶飞, 任浩, 管汉亮, 段金廒
南京中医药大学, 江苏省方剂高技术研究重点实验室, 江苏 南京 210046
摘要:
研究人体肠道细菌对柚皮苷的代谢。采集健康志愿者新鲜粪便,称重稀释后厌氧培养,通过反复涂布分离得到69种人体肠道细菌。采用UPLC-Q-TOF/MS研究柚皮苷经粪便混合菌及分离的单菌作用后的代谢产物,共鉴定出乙酰化结合产物、苷元及氢化的苷元3种代谢产物;其中对柚皮苷有特殊代谢活性的4种单菌采用16S rRNA进行了鉴定,分别为Enterococcus sp.30、Bacillus sp.46、Escherichia sp.54和Escherichia sp.63。结果显示肠道细菌对柚皮苷有代谢作用且由不同细菌完成。
关键词:    柚皮苷      肠道细菌      UPLC-Q-TOF/MS      代谢产物      16S rRNA     
Metabolism of naringin produced by intestinal bacteria
ZHANG Wei, JIANG Shu, QIAN Da-wei, SHANG Er-xin, QIAN Ye-fei, REN Hao, GUAN Han-liang, DUAN Jin-ao
Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China
Abstract:
Naringin has been reported to possess a wild range of biological activities. However, the route and metabolites of naringin produced by intestinal bacteria are not well understood. In this paper, different bacteria were isolated from human feces and their abilities to convert naringin to different metabolites were studied. Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) with automated data analysis software (MetaboLynxTM) was applied to fast analysis of naringin metabolites. Using MSE and mass defect filter techniques, three metabolites were detected and tentatively identified. The results indicated that acetylation, hydrolyzation and hydrolyzation with hydrogenation were the major metabolic pathways of naringin in vitro. Then, we studied the gene sequence of the 16S rRNA of the bacteria by extraction of genomic DNA of the strain, PCR amplification and clone of the 16S rRNA. The consequence proved that Enterococcus sp.30, Bacillus sp.46, Escherichia sp.54 and Escherichia sp.63 have the peculiar metabolism characteristic of naringin.
Key words:    naringin    intestinal bacteria    UPLC-Q-TOF/MS    metabolite    16S rRNA   
收稿日期: 2013-05-09
基金项目: 江苏省高校自然科学基金重大研究项目(10KJA360039);江苏省方剂高技术研究重点实验室建设项目(BM2010576).
通讯作者: 钱大玮, 段金廒
Email: qiandwnj@126.com
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