药学学报, 2008, 43(6): 657-663
引用本文:
陈士林;孙永巧;宋经元;李滢;李晨吉;胡松年;李西文;姚辉;张晓伟2;. 西洋参cDNA文库构建及表达序列标签(EST)分析[J]. 药学学报, 2008, 43(6): 657-663.
CHEN Shi-lin; SUN Yong-qiao; SONG Jing-yuan; LI Ying; LI Chen-ji; HU Song-nian; LI Xi-wen; YAO Hui; ZHANG Xiao-wei. Analysis of expressed sequence tags (EST) from Panax quinquefolium root[J]. Acta Pharmaceutica Sinica, 2008, 43(6): 657-663.

西洋参cDNA文库构建及表达序列标签(EST)分析
陈士林;孙永巧;宋经元;李滢;李晨吉;胡松年;李西文;姚辉;张晓伟2*
1. 中国医学科学院 药用植物研究所, 北京 100094; 2. 中国科学院 北京基因组研究所, 北京 100029
摘要:
为研究西洋参根的基因表达谱和挖掘其功能基因, 采用表达序列标签(EST)技术首次建立了四年生西洋参根的EST文库。通过BLAST与Gene Ontology分析获得与人参皂苷生物合成相关、 编码P450和糖基转移酶等的基因序列11个, 与生长素调节生长发育相关、 编码生长素响应因子4和生长素调节蛋白等的基因6个, 与水分胁迫相关、 编码蛋白dehydrin和DC2.15 like蛋白等的基因7个, 与编码抗氧化酶如peroxidase和catalase相关的基因6个。另外, 从西洋参根的EST文库获得抗病基因12个, 分别编码转录因子WRKY家族蛋白和ribonuclease蛋白家族, 62个EST是其他物种尚未报道的新基因。可见, EST技术作为功能基因组研究的重要手段可在西洋参功能基因的开发与研究中发挥重要作用, 这些基因的发现为进一步克隆基因全长、 研究基因功能、 改良西洋参品质、 阐明西洋参生长缓慢的分子机制等方面奠定了基础。
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Analysis of expressed sequence tags (EST) from Panax quinquefolium root
CHEN Shi-lin; SUN Yong-qiao; SONG Jing-yuan; LI Ying; LI Chen-ji; HU Song-nian; LI Xi-wen; YAO Hui; ZHANG Xiao-wei
Abstract:
To investigate the profile of gene expression in American ginseng (Panax quinquefolium L.) and discover its functional genes, for the first time, expressed sequence tags (EST) library of four-year-old American ginseng roots has been established. According to BLAST and Gene Ontology analysis, eleven genes, encoding cytochrome P450, glucosyltransferase, farnesyltransferase and cyclase family protein, are found to be associated with ginsenosides biosynthesis. Six other genes are obtained encoding auxin-regulated protein, auxin response factor 4 and auxin-repressed protein in the roots of American ginseng. In addition, thirteen expressed transcripts are stress-connected proteins and twelve expressed other transcripts are closely related to plant defense in four-year-old American ginseng roots. Furthermore, 62 genes no hit in BLAST and in Interproscan may be new genes. These results indicate EST is an useful tool for research on functional genomics of P.quinquefolium and it can be applied to the molecular modification of the ginsenosides biosynthetic pathway ultimately for improving the quality of American ginseng germplasm.
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通讯作者: 宋经元
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