药学学报, 2001, 36(7): 481-484
引用本文:
李庆平;陆泽安;饶曼人. 粉防己碱抑制血管平滑肌细胞胶原合成[J]. 药学学报, 2001, 36(7): 481-484.
LI Qing-ping; LU Ze-an; RAO Man-ren. DEPRESSIVE EFFECT OF TETRANDRINE ON COLLAGEN SYNTHESIS IN VASCULAR SMOOTH MUSCLE CELLS[J]. Acta Pharmaceutica Sinica, 2001, 36(7): 481-484.

粉防己碱抑制血管平滑肌细胞胶原合成
李庆平;陆泽安;饶曼人
南京医科大学心血管药理研究室,江苏南京210029
摘要:
目的 观察粉防己碱(Tet)对血管壁胶原沉积和血管平滑肌细胞胶原蛋白合成的影响。方法 用羟脯氨酸含量反映主动脉胶原沉积状况,用[3H]-脯氨酸参入反映细胞胶原蛋白合成。结果 与伪手术组相比,肾血管性高血压大鼠主动脉壁羟脯氨酸含量明显增加;粉防己碱可有效降低主动脉壁胶原含量,并呈浓度依赖性抑制[3H]-脯氨酸参入AngII或NE诱导的血管平滑肌细胞。结论 粉防己碱抑制血管平滑肌细胞胶原合成和动脉壁胶原沉积是其逆转血管重构作用的重要机制之一
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DEPRESSIVE EFFECT OF TETRANDRINE ON COLLAGEN SYNTHESIS IN VASCULAR SMOOTH MUSCLE CELLS
LI Qing-ping; LU Ze-an; RAO Man-ren
Abstract:
AIM To clarify whether collagen deposition and collagen synthesis are increased in renovascular hypertensive rats (RHR) and whether these are affected by treatment with tetrandrine (Tet). METHODS Collagen deposition was detected by electron microscope and evaluated by the amount of hydroxyproline in aorta of sham operated rats (Sham), renovascular hypertensive rats (RHR), and Tet treated RHR. The aortic vascular smooth muscle cells (AVSMCs) were isolated and cultured. The collagen synthesis in cultured AVSMCs was detected by [3H]-proline incorporation. RESULTS The ultrastructure of aorta suggests that AVSMCs in RHR had been transferred from contractile phenotype to synthetic phenotype. Compared with sham operated rats, the hydroxyproline content in aorta was increased by 36.6% in RHR, while it was reduced by 18.7% in Tet treated RHR (P<0.01,vs RHR). Tet (0.1-10 μmol·L-1) treatment in vitro induced a concentration dependent depression in [3H]-proline incorperation stimulated by NE or Ang II in AVSMCs from either Sham or RHR. CONCLUSION The depressive effects of tetrandrine on collagen synthesis and deposition in arteries may contribute to the regression of vascular remodeling.
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