药学学报, 2002, 37(5): 321-325
引用本文:
郭欲晓;罗谋伦;林志彬. 静注卡介苗建立免疫性胰岛素抵抗模型[J]. 药学学报, 2002, 37(5): 321-325.
GUO Yu-xiao; LUO Mou-lun; LIN Zhi-bin. ESTABLISHMENT OF IMMUNE INSULIN RESISTANCE MODEL IN THE RATS BY IV INJECTION OF BCG[J]. Acta Pharmaceutica Sinica, 2002, 37(5): 321-325.

静注卡介苗建立免疫性胰岛素抵抗模型
郭欲晓;罗谋伦;林志彬
北京大学基础医学院药理学系, 北京 100083
摘要:
目的建立并考察卡介苗诱导的胰岛素抵抗动物模型。方法用正糖钳实验方法及其他生化检验方法测定卡介苗每鼠10 mg iv后大鼠血糖、葡萄糖耐量、血清TNF-α、胰岛素刺激的葡萄糖代谢速率及其他生化指标的变化。结果卡介苗iv后第2,4及8周,动物的葡萄糖耐量均受到损伤,胰岛素刺激的葡萄糖代谢速率显著下降,血清TNF-α含量升高。结论卡介苗iv可造成稳定的胰岛素抵抗模型,其造模机理可能与升高大鼠血清TNF-α含量有关。
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ESTABLISHMENT OF IMMUNE INSULIN RESISTANCE MODEL IN THE RATS BY IV INJECTION OF BCG
GUO Yu-xiao; LUO Mou-lun; LIN Zhi-bin
Abstract:
AIMTo observe the stability of BCG-induced insulin resistance model. METHODSThe glucose tolerance, serum glucose, FFA, insulin, triglycerides, cholesterol, TNF-α and ALT level were measured. The change of GDR was measured by euglycemic clamp in model rats after given iv BCG 2, 4 and 8 weeks. RESULTSAfter 2, 4 and 8 weeks, the GIR and glucose tolerance of the animals deceased significantly. After 2, 4 and 8 weeks, BCG infusion resulted in a pronounced reduction in glucose tolerance and insulin-stimulated glucose disposal rate [GDR=GDR: (29±6) vs (13±7) mg·kg-1·min-1 2 weeks; (29±6) vs (11±7) mg·kg-1·min-1 4 weeks and (23±3) vs (16±3) mg·kg-1·min-1 8 weeks, respectively, P<0.01]. BCG infusion resulted in a pronounced increase in the weights of the liver [(6.2±0.9) vs (8.2±1.3) g, P<0.05] and spleens [(0.51±0.11) vs (1.4±0.4) g, P<0.01]. The histo-pathological results showed that BCG infusion resulted severe inflammation in the livers and spleens and the ratio of β/α in pancreas increased. The serum levels of triglyceride, FFA and glucose were unchanged, but the level of serum TNF-α [(543±60) vs (759±137) pg·mL-1, P<0.05] and insulin [(31±5) vs (36±5) mu·L-1, P>0.05] increased. CONCLUSIONThis novel model of immune insulin resistance is completely and constantly established.
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通讯作者: 林志彬
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