药学学报, 2002, 37(5): 329-333
引用本文:
张晶;郭继东;邢淑华;谷淑玲;戴体俊. 褪黑素对沙土鼠脑缺血再灌注损伤的保护作用[J]. 药学学报, 2002, 37(5): 329-333.
ZHANG Jing; GUO Ji-dongXING Shu-hua; GU Shu-ling; DAI Ti-jun. THE PROTECTIVE EFFECTS OF MELATONIN ON GLOBAL CEREBRAL ISCHEMIA-REPERFUSION INJURY IN GERBILS[J]. Acta Pharmaceutica Sinica, 2002, 37(5): 329-333.

褪黑素对沙土鼠脑缺血再灌注损伤的保护作用
张晶;郭继东;邢淑华;谷淑玲;戴体俊
1. 徐州医学院药理教研室, 江苏 徐州 221002; 2. 中国科学院上海生理研究所, 上海 200031; 3. 江苏省麻醉学重点实验室, 江苏 徐州 221002
摘要:
目的研究褪黑素(melatonin,MT)对沙土鼠脑缺血再灌注损伤的神经保护作用。方法用沙土鼠双侧颈总动脉结扎法建立全脑缺血再灌注损伤模型。用开场迷宫测试沙土鼠的定向运动活性的变化。用T迷宫测试沙土鼠的学习及工作记忆能力。光镜下观察缺血后d 7海马CA1区神经元组织形态学变化。结果缺血模型组沙土鼠定向运动活性较假手术组显著增高,学习及工作记忆能力降低。MT可降低沙土鼠的定向运动活性,提高沙土鼠的工作记忆能力;还可显著减轻海马CA1区锥体神经元的病理改变,且作用呈剂量依赖性。结论MT对沙土鼠全脑缺血再灌注损伤有保护作用。
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THE PROTECTIVE EFFECTS OF MELATONIN ON GLOBAL CEREBRAL ISCHEMIA-REPERFUSION INJURY IN GERBILS
ZHANG Jing; GUO Ji-dongXING Shu-hua; GU Shu-ling; DAI Ti-jun
Abstract:
AIMTo investigate the effects of melatonin (MT) on histology and behavioral tests during global cerebral ischemia-reperfusion in gerbils. METHODSGlobal cerebral ischemia was induced by occluding the bilateral common carotid arteries for 10 min in gerbils. Three doses of MT were administrated intraperitoneally 30 min prior to the onset of ischemia. Locomotor activity was measured by using the open field method 3 and 7 days after the ischemic episode. T maze test was carried out 4, 5 and 6 days after ischemia to assess the working memory of gerbils. Neuronal damage was assessed in CA1 pyramidal layer of gerbil hippocampus and evaluated 7 days after ischemia. RESULTS MT significantly reversed the locomotor activity increases, ameliorated learning and working memory deficit, and reduced the extent of CA1 hippocampal pyramidal cells injury after transient global cerebral ischemia in the Mongolian gerbil. CONCLUSIONMT provides significantly protective effect against both histological and behavioral consequences of global cerebral ischemia-reperfusion injury in gerbils.
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通讯作者: 邢淑华
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