Original articles
Jijun Fu, Bo Wu, Minyan Wei, Yugang Huang, Yi Zhou, Qiang Zhang, Lingran Du. Prussian blue nanosphere-embedded in situ hydrogel for photothermal therapy by peritumoral administration[J]. Acta Pharmaceutica Sinica B, 2019, 9(3): 604-614

Prussian blue nanosphere-embedded in situ hydrogel for photothermal therapy by peritumoral administration
Jijun Fua,b, Bo Wua,c, Minyan Weib, Yugang Huanga,b, Yi Zhoua,b, Qiang Zhangb, Lingran Dub
a The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510700, China;
b Key Laboratory of Molecular Target & Clinical Pharmacology, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436, China;
c Center of Pharmaceutical Research and Development, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436, China
Abstract:
To establish an injectable hydrogel containing Prussian blue (PB) nanospheres for photothermal therapy against cancer, PB nanospheres were prepared by one-pot synthesis and the thermosensitive Pluronic F127 was used as the hydrogel matrix. The PB nanospheres and the hydrogel were characterized by shape, particle size, serum stability, photothermal performance upon repeated 808 nm laser irradiation, as well as the rheological features. The effect of the PB nanospheres and the hydrogel were evaluated qualitatively and quantitatively in 4T1 mouse breast cancer cells. The retention, photothermal efficacy, therapeutic effects and systemic toxicity of the hydrogel were assessed in a tumor-bearing mouse model. The PB nanospheres had a diameter of about 150 nm and exhibited satisfactory serum stability, photo-heat convert ability and repeated laser exposure stability. The hydrogel encapsulation did not negatively influence the above features of the photothermal agent. The nanospherecontaining hydrogel showed a phase transition at body temperature and, as a result, a long retention time in vivo. The photothermal agent-embedded hydrogel displayed promising photothermal therapeutic effects in the tumor-bearing mouse model with little-to-no systemic toxicity after peritumoral administration.
Key words:    Prussian blue    Nanospheres    Hydrogel    Thermosensitive    Injectable    In situ    Photothermal   
Received: 2018-08-22     Revised:
DOI: 10.1016/j.apsb.2018.12.005
Funds: The research was supported by National Natural Science Foundation of China (81803463 and 51403043), the funding from Guangzhou Medical University (Grant No. B185004204, China) and Guangzhou City Council (1201410511, China) and Guangdong Provincial Council (15KAL10, China), Munici-pal College Research Program of Guangzhou Education Bureau (1201610202, China), Guangdong Natural Science Foundation (2015A030310037, China), and Guangdong Medical Science & Technology Research Foundation (A2017069, China), and the Scientific Research Foundation of Guangzhou Education Bureau (1201610085 and 1201430509, China) and Natural Science Foundation of Guangzhou Medical University (2014C09, China).
Corresponding author: Qiang Zhang, Lingran Du     Email:zqdodo@bjmu.edu.cn;dulingran@126.com
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Jijun Fu
Bo Wu
Minyan Wei
Yugang Huang
Yi Zhou
Qiang Zhang
Lingran Du

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