Original articles
Wenhao Wang, Zhengwei Huang, Yanbei Li, Wenhua Wang, Jiayu Shi, Fangqin Fu, Ying Huang, Xin Pan, Chuanbin Wu. Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proofof-concept study[J]. Acta Pharmaceutica Sinica B, 2021, 11(4): 1030-1046

Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proofof-concept study
Wenhao Wanga, Zhengwei Huanga, Yanbei Lia, Wenhua Wanga, Jiayu Shia, Fangqin Fub, Ying Huangb, Xin Pana, Chuanbin Wua
a School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China;
b College of Pharmacy, Jinan University, Guangzhou 511443, China
When nanoparticles were introduced into the biological media, the protein corona would be formed, which endowed the nanoparticles with new bio-identities. Thus, controlling protein corona formation is critical to in vivo therapeutic effect. Controlling the particle size is the most feasible method during design, and the influence of media pH which varies with disease condition is quite important. The impact of particle size and pH on bovine serum albumin (BSA) corona formation of solid lipid nanoparticles (SLNs) was studied here. The BSA corona formation of SLNs with increasing particle size (120-480 nm) in pH 6.0 and 7.4 was investigated. Multiple techniques were employed for visualization study, conformational structure study and mechanism study, etc. "BSA corona-caused aggregation" of SLN2-3 was revealed in pH 6.0 while the dispersed state of SLNs was maintained in pH 7.4, which significantly affected the secondary structure of BSA and cell uptake of SLNs. The main interaction was driven by van der Waals force plus hydrogen bonding in pH 7.4, while by electrostatic attraction in pH 6.0, and size-dependent adsorption was confirmed. This study provides a systematic insight to the understanding of protein corona formation of SLNs.
Key words:    Protein corona    Solid lipid nanoparticles    BSA corona-Caused aggregation    Nanoparticle-protein interaction    Size effect    Cell uptake    Medium pH    Conformational structure   
Received: 2020-07-06     Revised: 2020-08-24
DOI: 10.1016/j.apsb.2020.10.023
Funds: The authors would like to acknowledge the project grants from National Natural Science Foundation of China (81703431 and 81673375) and from the Natural Science Fund Project of Guangdong Province (2016A030312013, China). The authors would like to thank Haijiao Xie from Shiyanjia Lab (www. shiyanjia.com) for the AutoDock molecular docking technique.
Corresponding author: Ying Huang, huangy2007@jnu.edu.cn;Chuanbin Wu, wuchuanb@mail.sysu.edu.cn     Email:huangy2007@jnu.edu.cn;wuchuanb@mail.sysu.edu.cn
Author description:
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Wenhao Wang
Zhengwei Huang
Yanbei Li
Wenhua Wang
Jiayu Shi
Fangqin Fu
Ying Huang
Xin Pan
Chuanbin Wu

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