Original articles
Zibei Lin, Long Xi, Shaokui Chen, Jinsong Tao, Yan Wang, Xin Chen, Ping Li, Zhenping Wang, Ying Zheng. Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimodinduced psoriasis-like mice model[J]. Acta Pharmaceutica Sinica B, 2021, 11(4): 1047-1055

Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimodinduced psoriasis-like mice model
Zibei Lina, Long Xia, Shaokui Chena, Jinsong Taoa, Yan Wangb, Xin Chena, Ping Lib, Zhenping Wangc, Ying Zhenga
a State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, China;
b Beijing Hospital of Traditional Chinese Medicine, Affiliated with Capital Medical University, Beijing 100050, China;
c Department of Dermatology, School of Medicine, University of California, La Jolla, San Diego, CA 92093, USA
Abstract:
Psoriasis is an autoimmune inflammatory disease, where dendritic cells (DCs) play an important role in its pathogenesis. In our previous work, we have demonstrated that topical delivery of curcumin-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) could treat Imiquimod (IMQ)-induced psoriasis-like mice. The objective of this study is to further elucidate biofate of PLGA NPs after intradermal delivery including DCs uptake, and their further trafficking in psoriasis-like mice model by using fluorescence probes. Two-sized DiO/DiI-loaded PLGA NPs of 50 ±4.9 nm (S-NPs) and 226 ±7.8 nm (L-NPs) were fabricated, respectively. In vitro cellular uptake results showed that NPs could be internalized into DCs with intact form, and DCs preferred to uptake larger NPs. Consistently, in vivo study showed that L-NPs were more captured by DCs and NPs were firstly transported to skindraining lymph nodes (SDLN), then to spleens after 8 h injection, whereas more S-NPs were transported into SDLN and spleens. Moreover, FRET imaging showed more structurally intact L-NPs distributed in skins and lymph nodes. In conclusion, particle size can affect the uptake and trafficking of NPs by DCs in skin and lymphoid system, which needs to be considered in NPs tailing to treat inflammatory skin disease like psoriasis.
Key words:    Psoriasis    PLGA nanoparticles    Fluorescence    Dendritic cells    Fluorescence resonance energy transfer    Lymphoid organs    Uptake and trafficking    Biofate   
Received: 2020-06-11     Revised: 2020-07-31
DOI: 10.1016/j.apsb.2020.11.008
Funds: This work was supported by research grants from the Macau Science and Technology Development Fund (0013/2018/A1, China) and University of Macau (MYRG2017-00200-ICMS & MYRG2019-00032-ICMS, China). We thank to the members of FHS Animal Facility at the University of Macau for experimental and technical supports. We appreciate the help of Meng Xu on drawing graphic abstract.
Corresponding author: Ying Zheng, yzheng@umac.mo     Email:yzheng@umac.mo
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Zibei Lin
Long Xi
Shaokui Chen
Jinsong Tao
Yan Wang
Xin Chen
Ping Li
Zhenping Wang
Ying Zheng

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