Original articles
Payal Rana, Michael D. Aleo, Xuerong Wen, Stephen Kogut. Hepatotoxicity reports in the FDA adverse event reporting system database: A comparison of drugs that cause injury via mitochondrial or other mechanisms[J]. Acta Pharmaceutica Sinica B, 2021, 11(12): 3857-3868

Hepatotoxicity reports in the FDA adverse event reporting system database: A comparison of drugs that cause injury via mitochondrial or other mechanisms
Payal Ranaa, Michael D. Aleoa, Xuerong Wenb, Stephen Kogutb
a. Drug Safety Research & Development, Pfizer, Groton, CT 06340, USA;
b. University of Rhode Island, College of Pharmacy, Kingston, RI 02881, USA
Abstract:
Drug-induced liver injury (DILI) is a leading reason for preclinical safety attrition and post-market drug withdrawals. Drug-induced mitochondrial toxicity has been shown to play an essential role in various forms of DILI, especially in idiosyncratic liver injury. This study examined liver injury reports submitted to the Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS) for drugs associated with hepatotoxicity via mitochondrial mechanisms compared with non-mitochondrial mechanisms of toxicity. The frequency of hepatotoxicity was determined at a group level and individual drug level. A reporting odds ratio (ROR) was calculated as the measure of effect. Between the two DILI groups, reports for DILI involving mitochondrial mechanisms of toxicity had a 1.43 (95% CI 1.42–1.45; P < 0.0001) times higher odds compared to drugs associated with non-mitochondrial mechanisms of toxicity. Antineoplastic, antiviral, analgesic, antibiotic, and antimycobacterial drugs were the top five drug classes with the highest ROR values. Although the top 20 drugs with the highest ROR values included drugs with both mitochondrial and non-mitochondrial injury mechanisms, the top four drugs (ROR values > 18: benzbromarone, troglitazone, isoniazid, rifampin) were associated with mitochondrial mechanisms of toxicity. The major demographic influence for DILI risk was also examined. There was a higher mean patient age among reports for drugs that were associated with mitochondrial mechanisms of toxicity [56.1 ±18.33 (SD)] compared to non-mitochondrial mechanisms [48 ±19.53 (SD)] (P < 0.0001), suggesting that age may play a role in susceptibility to DILI via mitochondrial mechanisms of toxicity. Univariate logistic regression analysis showed that reports of liver injury were 2.2 (odds ratio: 2.2, 95% CI 2.12–2.26) times more likely to be associated with older patient age, as compared with reports involving patients less than 65 years of age. Compared to males, female patients were 37% less likely (odds ratio: 0.63, 95% CI 0.61–0.64) to be subjects of liver injury reports for drugs associated with mitochondrial toxicity mechanisms. Given the higher proportion of severe liver injury reports among drugs associated with mitochondrial mechanisms of toxicity, it is essential to understand if a drug causes mitochondrial toxicity during preclinical drug development when drug design alternatives, more clinically relevant animal models, and better clinical biomarkers may provide a better translation of drug-induced mitochondrial toxicity risk assessment from animals to humans. Our findings from this study align with mitochondrial mechanisms of toxicity being an important cause of DILI, and this should be further investigated in real-world studies with robust designs.
Key words:    Mitochondrial toxicity    FAERS database    Adverse event reporting    Drug-induced liver injury    Hepatotoxicity   
Received: 2020-12-24     Revised: 2021-03-02
DOI: 10.1016/j.apsb.2021.05.028
Funds: We thank Dr. Michael Leach and Dr. K. Nasir Khan for their support of our research. We also thank Dr. Mark Gosink, Dr. Seda Arat, and Lisa Marroquin for their review and assistance on this article.
Corresponding author: Payal Rana,E-mail:payal.m.rana@pfizer.com     Email:payal.m.rana@pfizer.com
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Payal Rana
Michael D. Aleo
Xuerong Wen
Stephen Kogut

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