Abstract: The study established the ¹H NMR-based fingerprinting and analyzed 8 batches of Huangqi injection solution. ¹H NMR-based fingerprinting of both primary and secondary metabolites of Huangqi injection were established, and the ¹H-¹H chemical shift correlation spectroscopy (COSY) and ¹H-¹³C chemical shift correlation spectroscopy (HSQC) were used to identify the chemical components in Huangqi injection solution. Coupled with similarity analysis and relative content determination, 8 batches of Huangqi injection solution were analyzed. Twenty-five metabolites (both primary and secondary) were identified, and the significant differences were found in the chemical composition among these Huangqi samples. The content and content variation of the primary metabolites were much higher than those of the secondary metabolites, which was the major cause of the uniformity of the Huangqi injections. The results on the quality variations of Huangqi injections in this study will serve as a basis for improving the quality control.